FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418499
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGCTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50970.3593234820750667No Hit
ATATTACGGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCAT50670.3572085704677973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45930.3237929670729412No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45490.32069109671561274No Hit
CCGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC43160.3042652832324873No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40700.2869230080528784No Hit
CCCTGGTTGTGTCCCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGC38210.2693692417125426No Hit
CTCCTAAAGTGCTCTTTTATTGGGCGTCTACCCGGGAGTCCGGAGTCCCT35560.2506875225149965No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34050.24004246742507399No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33650.23722258528204812No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30960.2182588778701994No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGCTATCCACCTTCCACTGTACTTTGGCCT30660.21614396626293003No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30550.21536849867359792No Hit
GTTTATTACTGTCAGCAATATTACGGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGCTGGAAATCAGACGAA29930.21099768135190788No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28780.20289052019070863No Hit
CTTTAGGAGGCTGTCCGGGCTTCTGCTGGAACCAACTTAAGAAGTGCTTA28440.20049362036913668No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27700.19527683840453888No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27270.19224546510078613No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACGCGCTGGCA26330.1856187420646754No Hit
GTCCCTGACCGATTCAGTGCCAGCGCGTCTGGGACAGATTTCACTCTCAC26140.18427929804673812No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25740.18145941590371228No Hit
GTACAGTGGAAGGTGGATAGCGCCCTCCAATCGGGTAACTCCCAGGAGAG24840.17511468108190417No Hit
GTCTCTGGTGCCTCCATGACAAACTTCTTCTGGATGTGGATTCGGCAGCC24670.1739162311711182No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23510.16573857295634328No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23280.16411714072410344No Hit
CTTTTATTGGGCGTCTACCCGGGAGTCCGGAGTCCCTGACCGATTCAGTGCCAGCGCGTCTGGGACAGATTTCA22570.15911184992023258No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22480.15847737643805176No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGCTATCCA22270.1569969383129632No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22250.15685594420581192No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22240.15678544715223627No Hit
CTTCTTAAGTTGGTTCCAGCAGAAGCCCGGACAGCCTCCTAAAGTGCTCTTTTATTGGGCGTCTACCCGGGAGTC22070.1555869972414503No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGTTCACCGTCAGGAAGAGTTGATTCTTGGACGCGTCAATGGA21470.15135717402691157No Hit
ATTCAGTGCCAGCGCGTCTGGGACAGATTTCACTCTCACCATCAACAGCC21330.15037021527685251No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACGGTGCT20980.1479028184017049No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCC20910.14740933902667536No Hit
CCCCAACACTAATAAGCACTTCTTAAGTTGGTTCCAGCAGAAGCCCGGAC19780.1394431719726274No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19570.13796273384753885No Hit
CAGTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGTTCACCGTCAGGAA19400.13676428393675286No Hit
GTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCTTGGCCGAACGTC19060.1343673841151809No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18590.13105402259712556No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18530.13063104027567168No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18460.13013756090064216No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18400.1297145785791883No Hit
CTCCCTGGTTGTGTCCCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTTTATAGTTCCTC18330.12922109920415875No Hit
GCTTAAGCCTTCGGAAACCCTGTCTCTCACTTGCACTGTCTCTGGTGCCT18040.12717668465046503No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18000.12689469643616247No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17790.1254142583110739No Hit
GTGCCAGCGCGTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTA17750.1251322700967713No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17690.12470928777531744No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17260.12167791447156466No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17240.12153692036441337No Hit
GTGCAAGTCCAGCCAGAGTATTTTTTATAGTTCCTCCATCAGTTCGAACC16950.11949250581071964No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16720.11787107357847978No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16500.11632013839981557No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16440.1158971560783617No Hit
ACCCGGGAGTCCGGAGTCCCTGACCGATTCAGTGCCAGCGCGTCTGGGACAGATTTCACTCTCACCATCAACAGC16340.11519218554260524No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAGCGCCCTCCAATCG16270.11469870616757573No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16120.11364125036394104No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16040.11307727393533587No Hit
GAGTGAAATCTGTCCCAGACGCGCTGGCACTGAATCGGTCAGGGACTCCG16010.11286578277460893No Hit
GTCAGGGACTCCGGACTCCCGGGTAGACGCCCAATAAAAGAGCACTTTAGGAGGCTGTCCGGGCTTCTGCTGGAA15850.1117378299173986No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15830.11159683581024732No Hit
CTATTACTGTGCTCGCTGGCCCACACAGGTGGGCCTGGGAAAGATTGTGTGGGGCCAGGGACTACTGGTCACCGT15800.11138534464952038No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15730.11089186527449084No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15660.11039838589946133No Hit
GTCTATTACTGTGCTCGCTGGCCCACACAGGTGGGCCTGGGAAAGATTGT15520.1094114271494023No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15510.10934093009582664No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACGGTGCTCCGTGGACGTT15470.10905894188152407No Hit
GCCAAGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCATCTGTC15350.10821297723861631No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15290.10778999491716243No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15280.1077194978635868No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15270.10764900081001115No Hit
CTAATAAGCACTTCTTAAGTTGGTTCCAGCAGAAGCCCGGACAGCCTCCTAAAGTGCTCTTTTATTGGGCGTCTA15240.10743750964928422No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15030.10595707152419565No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAGCGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15010.10581607741704435No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGCTATCCACCTTCCACTGTA15000.1057455803634687No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14900.10504060982771225No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14900.10504060982771225No Hit
CACTTGCACTGTCTCTGGTGCCTCCATGACAAACTTCTTCTGGATGTGGA14730.10384215991692627No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGCTATCCACCTTCCA14690.10356017170262369No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14660.10334868054189675No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14650.10327818348832112No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACGGTGCTCCGTGGACGTTCGGCCAAGGGACCAAG14580.10278470411329158No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14570.10271420705971594No Hit
GCCTCCTAAAGTGCTCTTTTATTGGGCGTCTACCCGGGAGTCCGGAGTCCCTGACCGATTCAGTGCCAGCGCGT14550.10257321295256464No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14420.10165675125608124No Hit
GACCGATTCAGTGCCAGCGCGTCTGGGACAGATTTCACTCTCACCATCAA14380.10137476304177867No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACGCGCTGGCA14300.1008107866131735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)