FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028598
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36180.3517409133597382No Hit
GTAATAAACAGCCGTGTCCTCAACTCTCAAACTGTTCATGTGAAGAAGCA34610.3364774187777927No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31570.30692262672103193No Hit
GTATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC30640.29788119362472026No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23620.22963295670417402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22790.22156372071499264No Hit
GTTTATTACTGTGCGAGAGTCTCCTACACCCCTTATGAGTACGGTGACTACCCCCGGTATATAGACTCCTGGGGC22520.21893878852574086No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22450.21825825055074968No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22390.2176749322864715No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21910.21300838617224613No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21890.21281394675082005No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21870.212619507329394No Hit
ATATAATACTTATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT21800.2119389693544028No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20780.20202255886167383No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19720.1917172695260928No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19280.18743960225471953No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18850.1832591546940593No Hit
CTAATACTCTGACTGGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGA18260.1775231917619906No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18130.17625933552272122No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17780.1728566456477652No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17070.1659540461871402No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16750.16284301544432325No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16710.16245413660147112No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16440.15982920441221937No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16400.15944032556936721No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16380.1592458861479412No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15370.14942669536592526No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAGTGGCAACTACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGA15210.1478711799945168No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15140.1471906420195256No Hit
GGACTGGAGTGGGTCTCGGTTATTCATAGCTCTGGTGTCACATATTACGC14800.14388517185528263No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCTCTTGATTTATAAGGCGT14530.14126023966603085No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14260.13863530747677907No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13870.13484373875897096No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13830.13445485991611883No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13830.13445485991611883No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13610.1323160262804322No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13590.13212158685900613No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13590.13212158685900613No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13560.13182992772686705No Hit
CTACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAGTGGGTCT13520.13144104888401495No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13310.12939943495904133No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13220.12852445756262407No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13150.1278439195876329No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13120.12755226045549378No Hit
GGTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAGCTACTA12890.12531620710909414No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12860.12502454797695503No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12780.12424679029125081No Hit
GTATATAGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCAT12530.12181629752342508No Hit
GTGTAGGAGACTCTCGCACAGTAATAAACAGCCGTGTCCTCAACTCTCAA12460.12113575954843389No Hit
ATACTTATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGA12360.12016356244130359No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGTAGTTGCCAC12330.11987190330916452No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12330.11987190330916452No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12200.11860804706989514No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12200.11860804706989514No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12150.11812194851633No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11980.11646921343420852No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11880.11549701632707823No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11790.11462203893066096No Hit
GAACACAGAGGACTCGCCATGGAATTTGGGCTGAGCTGGGTTTTCCTTGT11780.11452481921994793No Hit
CTCCAGAGACAGATCCAAGAATACGCTGCTTCTTCACATGAACAGTTTGAGAGTTGAGGACACGGCTGTTTATTA11780.11452481921994793No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTAAACCTTGATGGGACCCCA11730.11403872066638278No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11700.1137470615342437No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11580.11258042500568734No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11560.1123859855842613No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11500.11180266731998312No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11430.11112212934499192No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11390.11073325050213981No Hit
TTCTAAAGTAGACGCCTTATAAATCAAGAGGTTAGGGGCTTTCCCTGGCT11180.1086916365771662No Hit
GTCTCCTACAGATGCAGGCAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG11110.10801109860217499No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11080.1077194394700359No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11010.1070389014950447No Hit
GTCTCTGGAGATGGTAAATCGCCCCTTCACGGAGTCTGCGTAATATGTGACACCAGAGCTATGAATAACCGAGAC10940.10635836352005351No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10780.10480284814864504No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10740.10441396930579293No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10550.10256679480224538No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10420.10130293856297601No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10420.10130293856297601No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG10330.10042796116655876No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAG10330.10042796116655876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)