FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028598
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCAACTCTCAAACTGTTCATGTGAAGAAGCA33270.3234499775422468No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30380.29535348114618154No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30380.29535348114618154No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29860.290298056189104No Hit
GTATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC29500.28679814660343494No Hit
ATATAATACTTATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT24560.23877160951119875No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23880.23216066918271278No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22970.2233136755078272No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22640.22010542505429723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21420.20824462034730767No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21400.20805018092588165No Hit
GTTTATTACTGTGCGAGAGTCTCCTACACCCCTTATGAGTACGGTGACTACCCCCGGTATATAGACTCCTGGGGC20300.19735601274744846No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19900.19346722431892732No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18900.18374525324762445No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18400.178884267711973No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18290.17781485089412968No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17740.1724677668049131No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17610.1712039105656437No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17570.1708150317227916No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17290.16809287982282678No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17150.1667318038728444No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17130.16653736445141834No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16730.16264857602289717No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16420.1596347649907933No Hit
CTAATACTCTGACTGGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGA16300.15846812846223693No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15710.15273216553016825No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15390.14962113478735134No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCAGTGGCAACTACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGA15280.148551717969508No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14350.13951028487319633No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14290.13892696660891818No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14270.1387325271874921No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14160.1376631103696488No Hit
GGACTGGAGTGGGTCTCGGTTATTCATAGCTCTGGTGTCACATATTACGC14130.1373714512375097No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14030.13639925413037943No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13890.13503817818039698No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13850.13464929933754488No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAACCTCTTGATTTATAAGGCGT13670.1328993445447104No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13290.12920499553761527No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13260.1289133364054762No Hit
CTACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAGTGGGTCT13220.12852445756262407No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13150.1278439195876329No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13070.12706616190192865No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12960.12599674508408532No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12740.12385791144839871No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12480.12133019896985996No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTAAACCTTGATGGGACCCCA12330.11987190330916452No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12310.11967746388773846No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12290.1194830244663124No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12120.1178302893841909No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12060.11724697111991274No Hit
GGTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAGCTACTA12040.11705253169848667No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGTAGTTGCCAC11950.11617755430206943No Hit
GTGTAGGAGACTCTCGCACAGTAATAAACAGCCGTGTCCTCAACTCTCAA11930.11598311488064336No Hit
CTCCAGAGACAGATCCAAGAATACGCTGCTTCTTCACATGAACAGTTTGAGAGTTGAGGACACGGCTGTTTATTA11910.1157886754592173No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11780.11452481921994793No Hit
ATACTTATCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGA11750.11423316008780884No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11730.11403872066638278No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11460.11141378847713101No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11410.11092768992356586No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11410.11092768992356586No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11380.11063603079142677No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11370.11053881108071374No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11170.10859441686645317No Hit
GTCTCCTACAGATGCAGGCAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG11160.10849719715574015No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11140.10830275773431408No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10960.10655280294147958No Hit
GTCTCTGGAGATGGTAAATCGCCCCTTCACGGAGTCTGCGTAATATGTGACACCAGAGCTATGAATAACCGAGAC10920.10616392409862746No Hit
CTCCTACACCCCTTATGAGTACGGTGACTACCCCCGGTATATAGACTCCTGGGGCCAGGGAACCCTGGTCACCGT10880.10577504525577533No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10860.10558060583434929No Hit
GAACACAGAGGACTCGCCATGGAATTTGGGCTGAGCTGGGTTTTCCTTGT10760.104608408727219No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT10710.10412231017365384No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC10600.10305289335581054No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10470.10178903711654116No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10430.10140015827368905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT300.002108196770.9512570
GTCCTAA252.9616535E-453.6247861
TCTGGAG13200.044.07577570
GTATCAA13000.039.7029651
TGCGTGA350.001398468939.13328638
GTGGTAT2150.037.4126431
TCGCGGC400.002977165433.5497615
GTGCTAG601.6366801E-533.515491
TATCAAC15800.032.443832
ATCAACG16050.031.9338133
TCAACGC16250.031.5407834
AACGCAG16700.031.0966056
CAACGCA16650.030.9842435
CTACGCA553.7795468E-430.57719827
AATCGTT450.004724859330.52954339
CGCAGAG16850.030.4398678
AATGGTA1652.8200266E-630.10052970
AGAGTAC16850.030.05365611
TACGTAC450.005307782429.8118512
AATACGT450.005311572529.80749510
Produced by FastQC (version 0.11.5)