FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747789
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30390.40639806148525853No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28600.38246082785384644No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG22100.29553791243251776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20670.2764148710398254No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18810.2515415444731067No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17440.23322086845353435No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17200.23001140696105454No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17140.2292090415879346No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16630.2223889359164149No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAAGACCTACTTAAATTGGTATCAGCAGAAA16550.22131911541892163No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16420.2195806571104951No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16040.214499009747402No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15000.20059134327998943No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14400.19256768954878983No Hit
CATTAAGACCTACTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTA14380.19230023442441652No Hit
GTCTACATGGAGCTGGCCAGCCTGACATCTGAGGACACGGCCGTGTATTA14300.19123041392692322No Hit
ATATTACTGTCAACATTATGATAGTCTCCCACTCACTTTCGGCGGAGGGA14210.19002686586724332No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAGTAGGTCTTA14160.18935822805631No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13020.1741132859670308No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAAGAC12900.1725085552207909No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12810.17130500716111097No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12770.17077009691236433No Hit
ACATATTACTGTCAACATTATGATAGTCTCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12560.16796181810644445No Hit
GTTCAGTGGAGGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAACC12540.16769436298207113No Hit
CCCCTAAACTCCTCATCTACGATGCATCCAATTTGGAAACAGGGGCCCCA12270.16408371880303133No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12200.16314762586772472No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12040.16100798487273818No Hit
CATTATGATAGTCTCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11700.15646124775839174No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11640.15565888238527179No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11510.1539204240768452No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11460.1532517862659119No Hit
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGGCCAGCTCCATGTAGA11440.1529843311415386No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11300.15111214527092537No Hit
AGTCAGGACATTAAGACCTACTTAAATTGGTATCAGCAGAAACCAGGGAA11030.14750150109188556No Hit
GTGTATTACTGTGCTAGAGATGGAGGTCCTGGTGGATTAGGGGGCTACTACCTAAGGTACTGGGGCCAGGGAACC10960.14656540815657892No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10940.1462979530322056No Hit
ATCATAATGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGC10750.14375712935065907No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACCTCCA10700.14308849153972578No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG10520.1406813954203659No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10440.13961157492287263No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10430.13947784736068597No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10320.13800684417663273No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACCTCCA10150.1357334756194595No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10060.13452992755977955No Hit
ACCTAAGGTACTGGGGCCAGGGAACCCTGGTCAGCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG10010.13386128974884628No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC9960.13319265193791296No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT9950.1330589243757263No Hit
TTATGATAGTCTCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9640.12891336994793987No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9640.12891336994793987No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9560.1278435494504466No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9390.12557018089327338No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9270.12396545014703346No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9190.12289562964954018No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9110.1218258091520469No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9080.12142462646548692No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT9020.12062226109236696No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9000.12035480596799364No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8800.11768025472426046No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG8520.11393588298303399No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8500.11366842785866067No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8490.11353470029647401No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8470.11326724517210068No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8330.11139505930148745No Hit
ACCTTAGGTAGTAGCCCCCTAATCCACCAGGACCTCCATCTCTAGCACAG8290.1108601490527408No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8040.10751695999807433No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8030.10738323243588765No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT8000.10698204974932768No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCC7910.10577850168964774No Hit
CCATGTAGACTGTGCTCGTGGATACGTCCGCGGTAATGGTGACTCTGCCCTGGAACTTCTGTGCGTAGTTTGCAA7900.1056447741274611No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7850.1049761363165278No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7850.1049761363165278No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7830.10470868119215448No Hit
GTATTACTGTGCTAGAGATGGAGGTCCTGGTGGATTAGGGGGCTACTACCTAAGGTACTGGGGCCAGGGAACC7750.10363886069466119No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7740.10350513313247453No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7730.10337140557028787No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7680.10270276775935458No Hit
CCACTGAACCTTGATGGGGCCCCTGTTTCCAAATTGGATGCATCGTAGAT7680.10270276775935458No Hit
CCACCTATACTATCAACTGGGTGCGACAAGTCCCTGGACAAGGGCTTGAGTGGCTGGGAAAAATCATCCCTATC7670.10256904019716792No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7610.10176667482404796No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7600.1016329472618613No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7580.10136549213748797No Hit
GATTTTACTTTCACCATCAGCAACCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACATTATGATAGTCTC7520.10056312676436803No Hit
GTCTTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA7490.10016194407780805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)