FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747789
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28490.3809898246697932No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24330.3253591588001428No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21530.2879154413878781No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG20440.2733391371095322No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19520.26103620138835953No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16700.22332502885172154No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16430.21971438467268176No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15920.2128942790011621No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAAGACCTACTTAAATTGGTATCAGCAGAAA15700.2099522726330556No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14630.195643423479083No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14550.19457360298158974No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14380.19230023442441652No Hit
GTCTACATGGAGCTGGCCAGCCTGACATCTGAGGACACGGCCGTGTATTA14230.1902943209916166No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13920.18614876656383017No Hit
ATATTACTGTCAACATTATGATAGTCTCCCACTCACTTTCGGCGGAGGGA13850.18521267362852356No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13800.18454403581759027No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13680.18293930507135034No Hit
CATTAAGACCTACTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTA13590.18173575701167038No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAAGAC13510.18066593651417714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13510.18066593651417714No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12560.16796181810644445No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAGTAGGTCTTA12560.16796181810644445No Hit
CCCCTAAACTCCTCATCTACGATGCATCCAATTTGGAAACAGGGGCCCCA12500.1671594527333245No Hit
ACATATTACTGTCAACATTATGATAGTCTCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12280.16421744636521798No Hit
GTTCAGTGGAGGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAACC12120.16207780537023142No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12050.16114171243492484No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11560.1545890618877785No Hit
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGGCCAGCTCCATGTAGA11390.1523156933306053No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11380.15218196576841864No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11270.15071096258436537No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11210.14990859721124541No Hit
GTGTATTACTGTGCTAGAGATGGAGGTCCTGGTGGATTAGGGGGCTACTACCTAAGGTACTGGGGCCAGGGAACC11150.14910623183812546No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11100.14843759402719214No Hit
ATCATAATGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGC10870.145361860096899No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10710.14322221910191246No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACCTCCA10700.14308849153972578No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10420.1393441197984993No Hit
CATTATGATAGTCTCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10350.1384080268631927No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10160.13586720318164616No Hit
AGTCAGGACATTAAGACCTACTTAAATTGGTATCAGCAGAAACCAGGGAA9910.13252401412697967No Hit
ACCTAAGGTACTGGGGCCAGGGAACCCTGGTCAGCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG9900.132390286564793No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9870.13198910387823304No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9630.1287796423857532No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC9620.12864591482356655No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9570.12797727701263326No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG9560.1278435494504466No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9380.12543645333108672No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGTTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACCTCCA9370.12530272576890003No Hit
TTATGATAGTCTCCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9220.12329681233610017No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9090.12155835402767358No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT9000.12035480596799364No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8990.12022107840580698No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8810.11781398228644711No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT8680.11607552397802053No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG8650.11567434129146055No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8480.11340097273428734No Hit
GTCTTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA8430.11273233492335405No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8420.11259860736116739No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8400.11233115223679407No Hit
GATTTTACTTTCACCATCAGCAACCTGCAGCCTGAAGATTTTGCAACATATTACTGTCAACATTATGATAGTCTC8340.11152878686367411No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8180.10938914586868756No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8090.10818559780900762No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT8030.10738323243588765No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8010.10711577731151434No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7990.10684832218714102No Hit
ACCTTAGGTAGTAGCCCCCTAATCCACCAGGACCTCCATCTCTAGCACAG7910.10577850168964774No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7880.10537731900308778No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7780.10404004338122118No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7770.10390631581903452No Hit
CCATGTAGACTGTGCTCGTGGATACGTCCGCGGTAATGGTGACTCTGCCCTGGAACTTCTGTGCGTAGTTTGCAA7770.10390631581903452No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7710.10310395044591456No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7630.10203412994842127No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCC7520.10056312676436803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)