FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368301
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG49000.35810834019707655No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG39930.2918217555932503No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA36050.26346542171642057No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT32230.23554758784799543No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT31040.22685067101463788No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT29400.2148650041182459No Hit
GTATAATAATTGGCCCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28310.20689892063222934No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27760.20287933722185394No Hit
GTGTAATATGTGTTTGCACCACTAGTACTAACACGTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC26760.19557100374844424No Hit
CAGTAATATGCGCCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCATATA26160.19118600366439842No Hit
GTATATGCAAATGAACAGCCTGAGAGTCGACGACACGGGCGCATATTACT25850.1889204202876414No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGACTTGGTACAACCTGGGGGGTCCCTGAGACTCTCCTGCGCAGC25190.18409692019519097No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC24540.17934650343747466No Hit
TTATTATACTGCTGACAGTAATAAACTGTAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC24360.1780310034122609No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24330.1778117534080586No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC23650.17284208664613998No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22690.16582608651166666No Hit
GTAATATGCGCCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCATATACAGCGTGTTCTTGGAGTTGTCTCGGGA22390.16363358646964374No Hit
TGCATATACAGCGTGTTCTTGGAGTTGTCTCGGGACATGGTGAACCGGCC21680.15844466970352283No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA20540.15011316954383574No Hit
CATATTACACTGACTCCGTGAAGGGCCGGTTCACCATGTCCCGAGACAAC20510.14989391953963346No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC19710.1440472527609057No Hit
GTCCCAGACCCACTGCCACTAAACCTGGCTGGGATACCAGTGGCCCTGGT19230.14053925269366901No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18970.1386390859905825No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC18920.138273669316912No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT18530.13542341926228219No Hit
CTCCTGCGCAGCCTCTGGATTCATCTTTAACAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG18070.13206158586451372No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT18060.13198850252977962No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17900.13081916917403408No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT17590.12855358579727708No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17580.12848050246254297No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17540.12818816912360656No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT17000.12424166904796531No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT16960.12394933570902894No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGAGAAGGTGGTATAACCGGTCTCCCAGCGAT16830.12299925235748567No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGTTGT16550.12095291898493095No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16210.11846808560397164No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC16210.11846808560397164No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC16080.11751800225242837No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15980.1167871689050874No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15760.11517933554093726No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15240.1113790021347642No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC15230.11130591880003012No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT15060.11006350210955046No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA15060.11006350210955046No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15040.10991733544008227No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15020.10977116877061406No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG14980.10947883543167769No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG14980.10947883543167769No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14860.10860183541486852No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC14430.10545925202130232No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG14400.10524000201710003No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT14400.10524000201710003No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT14390.10516691868236595No Hit
GTACTAACACGTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC14310.10458225200449317No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14060.10275516863614075No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13980.10217050195826796No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA13880.10143966861092697No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA13880.10143966861092697No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA13850.10122041860672469No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGGCCAGTGTGAGGTGCAGCTGTTGGAGT13740.10041650192464961No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13700.10012416858571323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCC200.00756389651.39230339
GTATAGA200.00822272850.317951
CGTAGAG1450.044.08965328
GGTATCA7150.043.6323471
AGACGTA1500.042.52801525
ACGTAGA1550.041.21041527
ACGAGAC1650.040.66691622
CTTATAC1500.040.254361
GACGTAG1600.039.89044626
AATGGTA4850.039.7764870
CGTATGC1400.039.38390441
GAGACGT1650.038.6434624
GTATCAA19400.038.5598031
TCGTATG1450.037.89352840
CGAGACG1750.036.4299323
CCGTCTT1350.036.30703747
GCCGTCT1300.034.93839346
ATGCCGT1500.034.7255844
TATACAC1950.034.4054373
TATCAAC21700.034.009062
Produced by FastQC (version 0.11.5)