FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368301
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG49480.36161634026431316No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA35750.2612729216743977No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG34890.25498775488726527No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT29390.21479192078351184No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT27890.20382942057339723No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGACTTGGTACAACCTGGGGGGTCCCTGAGACTCTCCTGCGCAGC27560.20141767052717202No Hit
GTATAATAATTGGCCCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT27030.19754425378626486No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT26310.19228225368540985No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC25930.18950508696551416No Hit
GTGTAATATGTGTTTGCACCACTAGTACTAACACGTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC25600.18709333691928895No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC24960.18241600349630674No Hit
CAGTAATATGCGCCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCATATA24000.1754000033618334No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23880.17452300334502424No Hit
TTATTATACTGCTGACAGTAATAAACTGTAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC23650.17284208664613998No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22980.16794550321895546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22330.16319508646123915No Hit
GTATATGCAAATGAACAGCCTGAGAGTCGACGACACGGGCGCATATTACT22080.16136800309288674No Hit
GTAATATGCGCCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCATATACAGCGTGTTCTTGGAGTTGTCTCGGGA21460.15683683633937268No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21220.15508283630575437No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC20000.14616666946819448No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA19860.14514350278191712No Hit
CTCCTGCGCAGCCTCTGGATTCATCTTTAACAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG19010.13893141932951886No Hit
TGCATATACAGCGTGTTCTTGGAGTTGTCTCGGGACATGGTGAACCGGCC19000.13885833599478475No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT18780.13725050263063465No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18650.13630041927909137No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT18340.13403483590233434No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC18060.13198850252977962No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC17770.12986908582249082No Hit
CATATTACACTGACTCCGTGAAGGGCCGGTTCACCATGTCCCGAGACAAC17600.12862666913201115No Hit
GTCCCAGACCCACTGCCACTAAACCTGGCTGGGATACCAGTGGCCCTGGT17290.12636108575525415No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17250.12606875241631776No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC16870.12329158569642205No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGAGAAGGTGGTATAACCGGTCTCCCAGCGAT16480.12044133564179227No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT16150.11802958559556706No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15980.1167871689050874No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGTTGT15890.11612941889248053No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA15880.11605633555774643No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15680.11459466886306448No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15370.11232908548630748No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT15310.1118905854779029No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC15230.11130591880003012No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG15210.11115975213056191No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15120.11050200211795505No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT15020.10977116877061406No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14860.10860183541486852No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC14780.10801716873699574No Hit
GGGTTCCCTGGCCCCAGTAGTCCGAGAAGGTGGTATAACCGGTCTCCCAGCGATCTCTCACACAGTAATATGCGC14600.10670166871178197No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT14580.10655550204231379No Hit
CCCTGAGACTCTCCTGCGCAGCCTCTGGATTCATCTTTAACAACTATGCC14510.10604391869917511No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC14160.1034860019834817No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT13860.1012935019414588No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGGCCAGTGTGAGGTGCAGCTGTTGGAGT13830.1010742519372565No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA13790.10078191859832011No Hit
GTTTATTACTGTCAGCAGTATAATAATTGGCCCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13760.10056266859411782No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13730.10034341858991554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATC200.00709323352.23415445
GGTATCA7300.043.1599161
AATGGTA4900.041.51365770
GTATCAA16900.032.9228171
TCGTTGA802.8017785E-630.13228241
ATCGTTG802.8705927E-630.03863140
GCCGTTA450.005309088629.81104515
TTAGGGT450.005331915329.7848932
TACGGAG450.005331915329.7848932
TAATCGT704.1272193E-529.2732338
TGCGGTT605.6518806E-428.54728739
TCAACGC20100.027.8348984
ATCAACG20000.027.6390533
CAACGCA20400.027.4275655
TAGGTGG12650.027.2802055
ATAGGTG12550.027.2286224
AACGCAG20750.026.9688726
TCACGTA757.410912E-526.86335225
CCGTTAA500.0088787926.8309216
TAGTGTT500.00890098826.8171847
Produced by FastQC (version 0.11.5)