FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479897
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21480.44759604665167735No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20650.4303006686851554No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15240.31756814483107837No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14460.30131465710350347No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTACTACTACCTACATATGAG14450.301106279055714No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13980.29131251080961124No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12730.26526525483593355No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12240.25505473049425187No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12110.2523458158729894No Hit
GATTATTACTGCTGCTCATATGTAGGTAGTAGTACTTTCGTGGTATTCGG11250.23442530376309917No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG10840.2258818038037329No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10620.2212974867523656No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10360.21587965750984064No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCA10130.21108696241068395No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10000.2083780477894215No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTACTA9880.2058775112159484No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT9240.19254131615742545No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9060.18879051129721586No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8690.18108052352900728No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8620.17962187719448133No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8360.17420404795195638No Hit
GTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAA8350.17399566990416693No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG8340.1737872918563775No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8280.17253702356964098No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8140.1696197309005891No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8060.1679527065182737No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8040.16753595042269487No Hit
GTAATACACGGCCGTGTCAGCGGCGGTCACAGAGCTCAGCTGCAGGGAGA7830.163160011419117No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7640.159200828511118No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG7620.15878407241553918No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC7520.15670029193764495No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7450.155241645603119No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7400.15419975536417188No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7390.1539913773163825No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACACCA7360.15336624317301423No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7200.15003219440838347No Hit
TATGTAGGTAGTAGTACTTTCGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC7080.1475316578349104No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7010.14607301150038446No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6860.14294734078354313No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6760.14086356030564892No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6710.13982167006670182No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6470.1348205969197557No Hit
GTGTTGGTACCAGGAGACAAGGTTATAACTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA6450.13440384082417686No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC6330.1319033042507038No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6190.1289860115816519No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6140.12794412134270478No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6080.12669385305596825No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5900.12294304819575869No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT5890.12273467014796925No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5820.1212760238134433No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT5810.12106764576565388No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5680.11835873114439141No Hit
GTGTATTACTGTGCGAGAGAGATCCTGCACTGTAGTGGTGGTAGCTGTCCCTATTACTACTACGGTATGGAAGT5520.11502468237976064No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC5510.11481630433197124No Hit
ACCTACATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC5480.11419117018860298No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5330.11106549947176164No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT5300.11044036532839338No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGAGTTGGATCCG4970.10356388975134247No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC4940.1029387556079742No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC4890.1018968653690271No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4890.1018968653690271No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA4860.10127173122565884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT354.379331E-578.88196670
TGTCGGA150.002512830867.8781912
ATATGTC200.00752737751.45010837
CTCGTAG200.00778767751.0096327
CTCGATT200.00781342650.9670625
CGTTCGT200.00782955150.9404922
TACTGAT4250.050.34525370
GGTATCA4750.047.931921
TCTACTG3250.036.8979368
GTATCAA11850.036.705851
GTCTACT3200.036.08175767
TGTCTAC3300.034.4914166
CAACGGG400.002604280834.4743340
GCGAACT502.0403106E-433.9603323
AAAGCGA502.0428118E-433.95324720
CAAAGCG502.0440633E-433.94970719
TAAGACT502.0465683E-433.9426354
GTCTCGA400.002811982533.9390956
TCGTGTG400.002811982533.9390959
GTGTCTA3450.032.7958465
Produced by FastQC (version 0.11.5)