FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288875
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49180.3815730772960916No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48740.3781592474056833No Hit
ATTAATAACCGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAA42740.33160702162738825No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41700.3235379691591504No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC36850.2859082533216953No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35520.27558917660750654No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33700.2614683347880904No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31530.24463194646494033No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31480.24424401125012124No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30910.2398215498011832No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATATAATGATTTC30900.23974396275821935No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30050.23314906410629427No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29910.2320628455048007No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29820.23136456211812628No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29450.22849384152846478No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG29330.22756279701289886No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAATAACCGGTTGGCCTGGTATCAGCAGAGA27870.21623508874018038No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27300.21181262729124237No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27100.21026088643196586No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25960.2014159635340898No Hit
GTTATTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA25930.20118320240519832No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24360.1890020366598778No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACAT23930.18566579381243334No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTG23790.18457957521093976No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23510.18240713800795266No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTAAACCTTGATGGGACCCCA23470.18209678983609737No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23470.18209678983609737No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23260.18046746193385704No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCC23110.17930365628939965No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23010.17852778585976142No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22660.17581223935602755No Hit
TCATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC22480.17441567258267868No Hit
GTTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCGGTTATTA20950.16254485500921345No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCAATTTGGTGTCTGCTTTAGAAAGTGGGGTCCCAT20880.16200174570846668No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20250.1571137620017457No Hit
GACCAGGGAAAGCCCCTAAACTCCTGATCAATTTGGTGTCTGCTTTAGAA20130.1561827174861798No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19910.15447580254097565No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19630.15230336533798855No Hit
ACTTATTACTGCCAACAATATAATGATTTCCGAGGCCTCACTTTCGGCGG19440.15082921152167586No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19400.1505188633498206No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19220.14912229657647172No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19090.148113665017942No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18910.14671709824459314No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18620.14446707399864223No Hit
ATATAATGATTTCCGAGGCCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCAT18410.1428377460964019No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17610.1366307826592959No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17550.13616526040151294No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAATAA17350.13461351954223646No Hit
ACCTTGATGGGACCCCACTTTCTAAAGCAGACACCAAATTGATCAGGAGTTTAGGGGCTTTCCCTGGTCTCTG17320.134380758413345No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGAACCTGGGAGCCAGAG17110.13275143051110463No Hit
GCTTTAGAAAGTGGGGTCCCATCAAGGTTTAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC16860.13081175443700901No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16720.12972553583551547No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16560.12848414314809425No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA16500.12801862089031132No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15950.12375133352730094No Hit
TCCCTGGTCTCTGCTGATACCAGGCCAACCGGTTATTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACT15790.12250994083987973No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15780.1224323537969159No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCAATTTGGTGT15630.12126854815245854No Hit
CTGCTGATACCAGGCCAACCGGTTATTAATAGTCTGACTGGCCCGGCAAG15590.12095819998060324No Hit
CAATATAATGATTTCCGAGGCCTCACTTTCGGCGGAGGGACCACGGTGGA15520.12041509067985647No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15510.12033750363689263No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATATAATGATTTCCGAGGCCTCA15340.11901852390650762No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGAACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG15290.1186305886916885No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15030.11661332557462904No Hit
GATATAAGCCCGTGTCCTCAGGTCTCAGACTGCTCATTTGCAGAAACAGG14870.11537193288720783No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14860.115294345844244No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14840.11513917175831635No Hit
GCTGATACCAGGCCAACCGGTTATTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG14790.11475123654349723No Hit
GACTATTAATAACCGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCAATTTGGTGT14640.11358743089903986No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14560.11296673455532925No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14560.11296673455532925No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14510.11257879934051013No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14490.11242362525458248No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14480.11234603821161865No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14470.11226845116865483No Hit
CCCCTAAACTCCTGATCAATTTGGTGTCTGCTTTAGAAAGTGGGGTCCCA14460.112190864125691No Hit
GCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCAATTTGGTGTCTGCTT14390.11164775482494424No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14380.11157016778198042No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14350.11133740665308894No Hit
CATCAAGGTTTAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG14100.10939773057899332No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14060.10908738240713801No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC14030.10885462127824654No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13650.10590631364562118No Hit
GGTCTCTGCTGATACCAGGCCAACCGGTTATTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACT13430.10419939870041704No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13370.10373387644263408No Hit
GTCTATGGTCTCACTCTTCCGACTAATACCTGAGACCCACTCCAGGCCCTTCCCCGGAGGTTGCCGGACCCAGTG13350.10357870235670644No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13180.1022597226263214No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13120.10179420036853845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT200.007698923351.1626634
GTGGTAT3450.042.0880661
ACGAGAC3700.041.08234822
TCGTATG2950.040.87774740
CGTATGC3250.038.24933241
TCTCCGA4000.037.98937611
CGAGACC4100.037.0843923
ATCTCCG4100.037.06280510
GACCCTA4200.037.0562526
TCTTATA2200.036.8381772
GTATCAA31000.036.8183751
CACGAGA4050.036.69370321
ATCTCGT3200.036.38709337
ATGCCGT3550.036.20515444
CCCTAAG4400.036.1802128
CCCACGA4250.035.75886519
TAAGACA4400.035.5237831
CTTATAC4600.035.236521
TACACAT4700.034.4881445
CATCTCG3400.034.194236
Produced by FastQC (version 0.11.5)