FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288875
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47100.3654349723596159No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38960.30227911938706237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38020.29498593734846285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37860.2937445446610416No Hit
ATTAATAACCGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAA37230.2888565609543206No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36260.2813306177868296No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33740.26177868295994566No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30000.23276112889147513No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29420.2282610803995733No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29340.22764038405586268No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28450.22073513723208224No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATATAATGATTTC28340.21988167975948014No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27140.21057123460382116No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26480.2054504897682087No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA25870.20071768014741537No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25780.20001939676074096No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25470.1976141984288624No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTAAACCTTGATGGGACCCCA25430.19730385025700709No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG25170.19528658713994762No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24910.19326932402288818No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAATAACCGGTTGGCCTGGTATCAGCAGAGA24130.18721753467170982No Hit
GTTATTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA23740.18419163999612065No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23080.1790708951605082No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22540.17488119484046163No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21500.16681214237222383No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21450.16642420715740472No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21250.1648724662981282No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTG21200.16448453108330907No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACAT21140.16401900882552614No Hit
TCATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC21110.16378624769663466No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20950.16254485500921345No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20590.15975172146251576No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19690.1527688875957715No Hit
GTTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCGGTTATTA19320.14989816700610997No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19030.14764814276015906No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGAACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG18380.14260498496751042No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18280.14182911453787217No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18250.1415963534089807No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCAATTTGGTGTCTGCTTTAGAAAGTGGGGTCCCAT18150.14082048297934244No Hit
ATATAATGATTTCCGAGGCCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCAT18130.1406653088934148No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCC18100.14043254776452332No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17790.13802734943264475No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17450.1353893899718747No Hit
ACTTATTACTGCCAACAATATAATGATTTCCGAGGCCTCACTTTCGGCGG16630.12902725244884106No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAATAA16530.12825138201920278No Hit
ACCTTGATGGGACCCCACTTTCTAAAGCAGACACCAAATTGATCAGGAGTTTAGGGGCTTTCCCTGGTCTCTG16420.12739792454660073No Hit
GACCAGGGAAAGCCCCTAAACTCCTGATCAATTTGGTGTCTGCTTTAGAA16330.1266996411599263No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16190.12561342255843275No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16140.1252254873436136No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTTCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16140.1252254873436136No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16050.1245272039569392No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGAACCTGGGAGCCAGAG15990.12406168169915624No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA15790.12250994083987973No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15600.12103578702356707No Hit
GCTTTAGAAAGTGGGGTCCCATCAAGGTTTAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC15510.12033750363689263No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15140.1174667830472311No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14980.11622539035980992No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14910.11568228105906314No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14840.11513917175831635No Hit
CTGCTGATACCAGGCCAACCGGTTATTAATAGTCTGACTGGCCCGGCAAG14800.11482882358646106No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14780.1146736495005334No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14490.11242362525458248No Hit
GATATAAGCCCGTGTCCTCAGGTCTCAGACTGCTCATTTGCAGAAACAGG14070.10916496945010185No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14070.10916496945010185No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCAATTTGGTGT14040.10893220832121037No Hit
GCTGATACCAGGCCAACCGGTTATTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG14030.10885462127824654No Hit
TCCCTGGTCTCTGCTGATACCAGGCCAACCGGTTATTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACT13930.10807875084860828No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13790.10699253224711472No Hit
CAATATAATGATTTCCGAGGCCTCACTTTCGGCGGAGGGACCACGGTGGA13690.1062166618174765No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13690.1062166618174765No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13480.10458733391523614No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13400.10396663757152556No Hit
CCCCTAAACTCCTGATCAATTTGGTGTCTGCTTTAGAAAGTGGGGTCCCA13210.10249248375521287No Hit
GTCCCAGATCCACTGCCGCTAAACCTTGATGGGACCCCACTTTCTAAAGC13060.10132867811075551No Hit
GACTATTAATAACCGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAACTCCTGATCAATTTGGTGT13010.10094074289593638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)