FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178722
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA62340.5288778863888177No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50250.42630917213728087No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44240.3753217467732001No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA41050.348258537636525No Hit
GTAGTACACAGCTGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA38070.3229769190699758No Hit
CTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT36970.31364477798836365No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36650.31092997331007655No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34570.29328374290120995No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT33430.283612251234812No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33200.281660985372293No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31440.2667295596417137No Hit
ATATTATGGTACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT30210.25629452915954737No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30110.25544615269758264No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29810.2529010233116884No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA29700.25196780920352724No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29500.2502710562795977No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28760.24399307046105867No Hit
GTGTACTACTGTGTGAGACATAGCAGTTCGGGTTACTACTATTACGTGGA27000.22906164473047927No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT26760.2270255412217639No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26000.22057788011083188No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25520.21650567309340116No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24900.21124573902921978No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC24820.21056703785964798No Hit
GTTTATTACTGTCAACAATATTATGGTACTCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA24540.20819158376614672No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGCGACGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCA24370.20674934378080667No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23890.2026771367633759No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT23760.20157424736282178No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23480.1991987932693205No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCAC23470.199113955623124No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA22640.19207243098881668No Hit
AACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTACCCGGGAA22580.19156340511163786No Hit
CCATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGCGACGGTGAGAGTGAATTCTGTC22550.19130889217304844No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22220.1885092498485648No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21870.18553993223168821No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21460.18206158873763278No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21110.1790922711207562No Hit
GAATTCACTCTCACCGTCGCCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATGGTACT20640.17510490174952195No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20630.17502006410332546No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20530.1741716876413607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC20090.1704388312087159No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTA19480.16526373479073098No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGAATTCACTCTCACCGTCGCCAGCC19220.16305795598962267No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18590.1577131842792448No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18060.1532167890308317No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA18030.15296227609224228No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17730.15041714670634806No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16980.14405432324161252No Hit
CAGTAGTACACAGCTGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA16960.1438846479492196No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16930.14363013501063016No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16770.14227273267148657No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16740.14201821973289716No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG16690.14159403150191477No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16660.14133951856332536No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16480.13981244093178882No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA16210.13752182448448405No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT15990.13565539626816162No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC15600.13234672806649916No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15140.12844419634146134No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15120.1282745210490684No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14990.12717163164851422No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14760.12522036578599535No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14540.12335393756967292No Hit
GTCTCTGGTGGCTCCATCAGCAATAGTCCTTACTTCTGGGGCTGGATCCG14240.12080880818377869No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14170.12021494466040339No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC13910.11800916585929506No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13780.1169062764587409No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC13700.11622757528916912No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13440.1140217964880608No Hit
GTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA13330.11308858237989959No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13200.11198569297934542No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13170.11173118004075601No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13160.11164634239455952No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13060.11079796593259478No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13020.1104586153478089No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12980.110119264763023No Hit
TCTTCAGCCTGCAGGCTGGCGACGGTGAGAGTGAATTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12870.1091860506548618No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12850.10901637536246883No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12820.10876186242387942No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12730.10799832360811115No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12700.10774381066952174No Hit
CCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG12600.106895434207557No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTACTCATT12440.10553803186841343No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12430.10545319422221695No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATGGTACTCCTC12360.10485933069884162No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12330.10460481776025221No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12220.10367160365209101No Hit
GTACTACTGTGTGAGACATAGCAGTTCGGGTTACTACTATTACGTGGACGTCTGGGGCAAAGGGACCACGGTCAT12150.10307774012871568No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12100.1026535518977333No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG12040.10214452602055446No Hit
GTCTGGGACAGAATTCACTCTCACCGTCGCCAGCCTGCAGGCTGAAGATG11970.10155066249717916No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11960.10146582485098267No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11890.10087196132760735No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11810.10019326015803558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)