FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017879
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCCATGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT78620.7723904314756469No Hit
CTATAATACTGCTGACAGTAATACACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC60920.598499428714022No Hit
GTTTTATACAGCTCCAACAATCAGAACTACTTAGCTTGGTACCAGCAGAA48550.4769722137896548No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37870.3720481511063692No Hit
GTATTACTGTCAGCAGTATTATAGTACTCCCATGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA37830.37165517708882884No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT32340.3177194931814096No Hit
CAGTAATACACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA31790.31231610044022917No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30470.29934795786139606No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27730.27242923765987903No Hit
GTAATAAACGGCCGTGTCAGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA25850.25395945883548043No Hit
GTGTATTACTGTCAGCAGTATTATAGTACTCCCATGTATACTTTTGGCCA24240.23814225462947952No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTGATTGTTGGAGCTGTATAAAACACT24100.23676684556808814No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23120.22713898213834846No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22890.22487938153749118No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22230.21839531024807468No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21980.2159392226384472No Hit
GTAATACACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA21770.21387610904636012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21470.21092880391480714No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20210.1985501223622847No Hit
GTATAAAACACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA19670.19324497312548938No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18540.1821434571299732No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18410.18086629157296694No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17300.16996126258622096No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17270.16966653207306567No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17110.16809463600290409No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17030.16730868796782328No Hit
CTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGGTATCTATCATAGTGGGACCAC16740.16445962634065542No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16280.15994042513894086No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16270.15984218163455577No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15730.15453703239776045No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15590.15316162333636904No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15400.1512949967530522No Hit
GTCTCTGATTACTCCGTCAGCAGTGGTCACTACTGGGGCTGGATCCGGCA15400.1512949967530522No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15200.1493301266653502No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14560.14304254238470387No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14530.14274781187154859No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14480.14225659434962307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13520.1328252179286536No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13510.1327269744242685No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTGTTTTATA13510.1327269744242685No Hit
CACCTGCACTGTCTCTGATTACTCCGTCAGCAGTGGTCACTACTGGGGCT13310.13076210433656654No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12850.12624290313485198No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAGTGTTTTATACAGCTCCAA12500.12280438048137353No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTATACATGGGAGTACTATAATACTG12450.12231316295944802No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC12400.12182194543752253No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA12320.12103599740244174No Hit
GTATACATGGGAGTACTATAATACTGCTGACAGTAATACACTGCCACATC12220.12005356235859074No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC12090.11877639680158447No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12060.11848166628842917No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11900.1169097702182676No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11900.1169097702182676No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11620.11415895209548482No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11580.11376597807794445No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT11360.11160462098147225No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT11270.11072042944200637No Hit
GTTTATTACTGTGCGAGACATACGAGTCATAGTGGGTCATTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT11130.10934502038061499No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11080.10885380285868951No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11000.1080678548236087No Hit
GGGTTCCCTGGCCCCAGTAGTCAAATGACCCACTATGACTCGTATGTCTCGCACAGTAATAAACGGCCGTGTCAG10980.10787136781483851No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT10950.1075766373016832No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10930.10738015029291301No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG10890.1069871762753726No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10700.10512054969205574No Hit
CATGTATACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG10670.10482581917890044No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTGTATTACTGTCAGCAGTATTATAGTACT10630.10443284516136005No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10610.10423635815258986No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10280.1009943225078816No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10190.10011013096841569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT200.00706120652.29300748
CTTACGT200.007779409651.02748525
GCTAGGT200.00781577950.9673168
GGTATCA9350.043.255621
GTGGTAT2800.041.2693941
TGGTATC2800.040.0496832
GTATCAA20800.035.2937361
TCCGAGA5150.034.3722770
TACTGAT10950.033.5754470
GACAGGT902.2749555E-730.2102761
ACAGCGA450.004980148730.2028528
CAAGCGG450.004983739530.198414
ACGAACT19650.030.0355555
CGAACTG19900.029.90764256
ACAGGTA1600.029.738241
GAACTGT20150.029.59570357
AACGAAC20200.029.13585954
GCTTGTA1052.6961061E-829.1313361
TGTTAGA9900.028.88392470
TATCAAC25400.028.6301232
Produced by FastQC (version 0.11.5)