FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927062
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59340.6400866392970481No Hit
CTACAAACTCCGGCCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACG42020.45325986827202497No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT41630.4490530298944407No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40510.4369718530152244No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39850.42985258806854343No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39550.4266165585473248No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA37280.40213060183677035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35590.3839009688672386No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34950.3769974392219722No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28690.30947228987920977No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28390.3062362603579912No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28220.30440251029596727No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27780.29965633366484656No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT27540.2970675100478717No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27190.29329214227311656No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGATACAACTATTTGGATTGGTACATGCAGAAGCCAGGCCAGTCTC26460.2854178037714845No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25150.2712871415288298No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24800.2675117737540747No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24450.26373640597931963No Hit
CCCCTGGAGAGCCGGCCGCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA24380.2629813324243686No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24370.2628734647736613No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24320.26233412652012483No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG23740.25607780277910214No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23560.254136185066371No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23330.2516552291001033No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC23230.25057655259303047No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA22660.24442809650271502No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCGGCCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA22610.2438887582491786No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT22290.24043699342654537No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21920.2364458903503757No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21370.23051316956147488No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG21190.22857155184874367No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG21030.22684566943742707No Hit
CTCCTACATAGTAATGGATACAACTATTTGGATTGGTACATGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGAT20520.22134441925135534No Hit
GTAATGGATACAACTATTTGGATTGGTACATGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATTTGGGTT20020.21595103671599095No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19610.21152846303699213No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19440.20969471297496825No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19370.2089396394200172No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19050.205487874597384No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18810.20289905098040908No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGGCCTGGCTTCTGCATGTA18320.19761353609575194No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17800.19200441825897296No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17700.19092574175190008No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA17410.18779757988138873No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17200.18553235921653569No Hit
GTACATGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA17190.18542449156582838No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17160.18510088861370652No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16370.17657934420783075No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCTCTCTCCCTGCCCGTCACCC16300.17582427065287975No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16280.17560853535146517No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCGGCCACTTTCGGCGGAGGGA16100.173666917638734No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16060.17323544703590482No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15830.1707544910696372No Hit
AGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT15660.1689207410076133No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15630.16859713805549142No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCGGCCACTTTCGGCGGAGGGA15570.1679499321512477No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15570.1679499321512477No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCTCTCTCC15410.1662240497399311No Hit
ATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGCGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGAGGAGA15170.1636352261229562No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA15010.16190934371163956No Hit
CTCCAGGGGTGACGGGCAGGGAGAGAGGAGACTGAGTCATCACAATATCC14950.16126213780739582No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCTCT14870.16039919660173754No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC14830.15996772599890838No Hit
GGATACAACTATTTGGATTGGTACATGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAAT14780.15942838774537194No Hit
GGTTTATTACTGCATGCAAGCTCTACAAACTCCGGCCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA14720.15878118184112822No Hit
AGCCAGGCCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC14490.1563002258748606No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14470.156084490573446No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14470.156084490573446No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT14410.15543728466920229No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14350.15479007876495854No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14280.15403500521000754No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14270.15392713755930024No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14000.15101471099020347No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13950.150475372736667No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13920.15015176978454514No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13790.14874949032535043No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13520.14583706375625363No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13460.14518985785200988No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12870.1388256664602799No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12830.13839419585745075No Hit
GGTACATGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATTTGGGT12530.1351581663362321No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG12510.1349424310348175No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA12460.13440309278128107No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12310.13278507802067177No Hit
CCTCTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCGCCATCTCCTGCAG12160.13116706326006242No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12160.13116706326006242No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12050.12998051910228225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11970.12911757789662395No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11940.1287939749445021No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11860.1279310337388438No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11660.12577368072469802No Hit
ACATAGTAATGGATACAACTATTTGGATTGGTACATGCAGAAGCCAGGCC11630.12545007777257616No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11570.12480287186833242No Hit
CTCCTGCAGGTCTAGTCAGAGCCTCCTACATAGTAATGGATACAACTATTTGGATTGGTACATGCAGAAGCCAGG11430.1232927247584304No Hit
GACTAGACCTGCAGGAGATGGCGGCCGGCTCTCCAGGGGTGACGGGCAGG11420.1231848571077231No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11270.12156684234711378No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11180.1205960334907482No Hit
GTCTCCTCTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCGCCATCTCCT11170.12048816584004089No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11160.1203802981893336No Hit
GGGATATTGTGATGACTCAGTCTCCTCTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCGCCATCTCCTGCAGGT11110.11984095993579717No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCGCCATCTCCTGCAGGTCTA11040.11908588638084615No Hit
ATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCGGCCACTTTC10730.11574198920892022No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTGGCCGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCT10730.11574198920892022No Hit
AGTCAGAGCCTCCTACATAGTAATGGATACAACTATTTGGATTGGTACATGCAGAAGCCAGGCCAGTCTCCACAG10660.1149869156539692No Hit
GTGGAGACTGGCCTGGCTTCTGCATGTACCAATCCAAATAGTTGTATCCA10620.11455544505114006No Hit
CAACTATTTGGATTGGTACATGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGC10500.1132610332426526No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10460.11282956263982344No Hit
CCTACATAGTAATGGATACAACTATTTGGATTGGTACATGCAGAAGCCAG10400.11218235673557972No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10370.11185875378345785No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT10360.11175088613275055No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG10320.11131941552992142No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10300.11110368022850683No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC10110.10905419486506836No Hit
ACCCTGGTCCCTCCGCCGAAAGTGGCCGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGC10090.10883845956365379No Hit
CCCTGGTCCCTCCGCCGAAAGTGGCCGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT10040.10829912131011733No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT10030.10819125365941006No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9920.10700470950162988No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9720.10484735648748413No Hit
CCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA9590.10344507702828938No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCTCTCTCCCTGCCCGTCACCCCTGGAGAGCCGG9580.10333720937758208No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCC9550.10301360642546022No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9470.1021506652198019No Hit
GCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGT9340.10074838576060717No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG9330.10064051810989988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGA150.00244570968.34836628
GTGGTAT3000.046.6466981
CGCTCGG306.697892E-445.4476111
TGGTATC3100.045.1151662
GTATCAA23900.040.5582051
GATGCGT350.001309636339.65885543
GGTATCA9200.039.325691
GTTAGAC451.0731956E-437.885273
TATCAAC27450.035.167742
ATCAACG28550.034.037033
TCAACGC28550.033.671484
CAACGCA28950.033.206245
AACGCAG29400.032.697986
GTATTAT751.8145947E-631.8562831
ACGCAGA30650.031.475687
GGAGTAA900.004149641431.34667870
CGCAGAG31000.031.2302748
TCTAAGG553.48013E-431.0104242
ACGGGTT450.00489583430.30821416
AGAGTAC32050.030.2038711
Produced by FastQC (version 0.11.5)