FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581847
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTAGCCCGGGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCAT74340.46995695538190485No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67790.4285496637791139No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67490.426653146606467No Hit
AATTAGGACAGCCTCCAAAGCTGCTCATTCACTGGGCATCTATCCGAGAA53300.33694788434026807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48300.30533926479615286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43520.2751214245119787No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTAATGGTGAGAGTGAAATCTGTC42070.2659549248441853No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39920.25236321844021575No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39300.24844374961674548No Hit
CTCCAAAGCTGCTCATTCACTGGGCATCTATCCGAGAATCCGGGGTCTCT39200.2478115772258632No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38640.24427141183692228No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38490.24332315325059883No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38240.24174272227339305No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37470.23687499486359934No Hit
GTTTATTACTGTCAGCAATATTATAGTAGCCCGGGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAA36030.22777171243489416No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35610.22511658839318846No Hit
CCATTAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAA35270.22296720226418865No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34700.21936381963615947No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34360.2172144335071597No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34240.2164558266381009No Hit
GTTTTCTACAGCTCCAACAGTAAGAACTACTTAAGTTGGTATCAGCACAA34230.2163926093990127No Hit
GCTTTGGAGGCTGTCCTAATTTGTGCTGATACCAACTTAAGTAGTTCTTACTGTTGGAGCTGTAGAAAACACT28220.1783990487069862No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28100.17764044183792743No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTCTACAGCTCCAA27990.1769450522079569No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27750.17542783846983936No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26930.1702440248646045No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26870.16986472143007508No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25150.15899135630689948No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24970.1578534460033113No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24880.15728449085151724No Hit
GATTTCACTCTCACCATTAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAGC24860.15715805637334077No Hit
GTCCTAATTTGTGCTGATACCAACTTAAGTAGTTCTTACTGTTGGAGCTG24680.15602014606975265No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGC24170.15279606687625288No Hit
ACCATATACTACGCAGACTCTGTGGAGGGCCGATTCACCATCTCCAGAGACAACGCCAGGAAGTCACTGTATCTG23980.1515949393335765No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTCTA23950.1514052876163118No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23280.14716973259740038No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTAATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22730.1436927844475477No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22430.1417962672749008No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21860.13819288464687166No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21740.1374342777778129No Hit
CACCATTAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAGCCCGGGGACGTT21600.13654923643057768No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21500.13591706403969536No Hit
GTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21440.13553776060516598No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21280.13452628477975428No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCCCTTGGCCGAACGTC21070.13319872275890146No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20780.13136542282534278No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20430.1291528194572547No Hit
GTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC20380.12883673326181355No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20310.12839421258819594No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19850.12548621959013734No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19650.12422187480837274No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19530.12346326793931399No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19520.12340005070022575No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19480.12314718174387283No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19450.12295753002660814No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19230.12156675076666708No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18940.11973345083310838No Hit
TTAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATAT18660.11796336813863795No Hit
GTATATGGTACTACCACTACTACTGATGTATGAAATCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT18650.11790015089954971No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18580.1174576302259321No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18580.1174576302259321No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18310.11575076477054987No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18230.11524502685784403No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18120.1145496372278735No Hit
GTCAGAGACCCCGGATTCTCGGATAGATGCCCAGTGAATGAGCAGCTTTGGAGGCTGTCCTAATTTGTGCTGATA17940.11341172692428535No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17910.11322207520702064No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATTAGTAGCC17750.11221059938160897No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17570.11107268907802083No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCC17280.10923938914446213No Hit
GTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTCTACAGCTCCAACAGTAAGAACTA17230.108923302949021No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17230.108923302949021No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17130.10829113055813867No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT16930.10702678577637406No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16880.10671069958093293No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16780.1060785271900506No Hit
GCTCATTCACTGGGCATCTATCCGAGAATCCGGGGTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT16680.10544635479916831No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16580.10481418240828602No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16550.10462453069102133No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16450.103992358300139No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16270.10285444799655087No Hit
GTAGAAAACACTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16040.10140045149752157No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15890.10045219291119811No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15880.10038897567210987No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15870.10032575843302166No Hit
GCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC15870.10032575843302166No Hit
GCTCCAACAGTAAGAACTACTTAAGTTGGTATCAGCACAAATTAGGACAGCCTCCAAAGCTGCTCATTCACTGGG15830.10007288947666874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3300.045.0286341
TCGTATG3300.043.9421140
ACATGCG3550.042.40794427
ATCTCGT3350.041.09110337
GTGGTAT4200.040.6812131
ATGCCGT3700.040.29079444
CCCACGA3700.039.71240619
GATCTCG3550.038.74156636
ACGAGAC3800.038.67590322
GTATGCC4050.038.4493742
GCCGTCT3800.038.40564746
CACGAGA3850.038.1721
ATCTCCG3850.038.15793210
ATGCGCA3900.037.7498829
CATGCGC4050.037.18181228
TACGTAT700.001917473236.6972270
GTATCAA41400.036.4008451
CGAGACA4250.036.19379823
CCGTCTT4350.034.38504847
GATCGAA701.0478561E-634.17870722
Produced by FastQC (version 0.11.5)