FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581847
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTAGCCCGGGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAACTGTGGCTGCACCAT70300.4444171907902597No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66880.42279689502208495No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55280.3494648976797377No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51750.3271492122815923No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTAATGGTGAGAGTGAAATCTGTC47230.2985750202137122No Hit
AATTAGGACAGCCTCCAAAGCTGCTCATTCACTGGGCATCTATCCGAGAA43210.2731616901002436No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41920.26500666625786184No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41590.26292049736795026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39950.25255287015748046No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39220.24793801170403962No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36040.2278349296739824No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35780.22619128145768838No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35170.22233502987330633No Hit
GTTTATTACTGTCAGCAATATTATAGTAGCCCGGGGACGTTCGGCCAAGGGACCACGGTGGAAATCAAACGAA34250.21651904387718912No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34150.21588687148630684No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34040.2151914818563363No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33410.21120879579377777No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33300.21051340616380726No Hit
CTCCAAAGCTGCTCATTCACTGGGCATCTATCCGAGAATCCGGGGTCTCT32000.2022951650823373No Hit
CCATTAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAA31570.1995768238015434No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30340.19180110339369105No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTAATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA30230.19110571376372051No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28710.18149669342230948No Hit
GTTTTCTACAGCTCCAACAGTAAGAACTACTTAAGTTGGTATCAGCACAA28620.18092773827051542No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27670.17492210055713353No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27260.17233019375451608No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26510.16758890082289882No Hit
GCTTTGGAGGCTGTCCTAATTTGTGCTGATACCAACTTAAGTAGTTCTTACTGTTGGAGCTGTAGAAAACACT26480.1673992491056341No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26120.16512342849845782No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24900.1574109253296937No Hit
GATTTCACTCTCACCATTAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAGC23780.1503305945518119No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23230.14685364640195925No Hit
GTCCTAATTTGTGCTGATACCAACTTAAGTAGTTCTTACTGTTGGAGCTG22960.14514678094657701No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCGTGGTCCCTTGGCCGAACGTC22870.14457782579478293No Hit
ACCATATACTACGCAGACTCTGTGGAGGGCCGATTCACCATCTCCAGAGACAACGCCAGGAAGTCACTGTATCTG21680.13705497434328354No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21610.1366124536696659No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTCTACAGCTCCAA21540.1361699329960483No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGCCAGAGTGTTTTCTA21380.13515845717063663No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21220.13414698134522493No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20990.13269298484619563No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20960.13250333312893092No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20850.1318079434989604No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20660.130606815956284No Hit
GTCTCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20040.12668734713281374No Hit
GTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC19910.12586552302466675No Hit
CACCATTAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTAGCCCGGGGACGTT19760.12491726443834326No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19730.12472761272107859No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAGCTGCAAGTCCAGC19520.12340005070022575No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACCCCG19480.12314718174387283No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19260.12175640248393177No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18840.11910127844222608No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18680.1180898026168144No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18450.1166358061177851No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18440.11657258887869686No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18270.11549789581419695No Hit
GTATATGGTACTACCACTACTACTGATGTATGAAATCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT18170.11486572342331465No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17800.11252668557705012No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17720.11202094766434428No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17620.11138877527346197No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACCCCGGATTCTCGGAT17560.11100947183893259No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17500.1106301684044032No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17430.11018764773078558No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTAATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17400.10999799601352091No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17140.10835434779722691No Hit
TCTTCAGCCTGCAGGCTACTAATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGAGACC17000.1074693064499917No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16970.10727965473272699No Hit
GTCAGAGACCCCGGATTCTCGGATAGATGCCCAGTGAATGAGCAGCTTTGGAGGCTGTCCTAATTTGTGCTGATA16740.10582565823369769No Hit
TTAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATAT16670.10538313756008008No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16660.10531992032099186No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16610.1050038341255507No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16590.10487739964737423No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16540.10456131345193309No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16530.10449809621284485No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16490.10424522725649193No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16480.1041820100174037No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16250.10272801351837439No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16040.10140045149752157No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16030.10133723425843333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAA200.007656106351.2354524
GTGGTAT4900.041.786491
GTATCAA43400.037.6640051
TATCAAC50250.032.24482
ATCAACG50350.031.8349693
TCAACGC51200.031.3730684
ATAGCGG1850.030.92470763
CAACGCA52250.030.7435745
AACGCAG52450.030.6263476
CGCAGAG55600.028.8912188
AGAGTAC57350.028.12944811
CAGAGTA57350.028.12944810
GCAGAGT57650.027.9821829
AATGGTA4900.026.61980270
TCTCGCG7250.026.08740670
TAGCGGC2200.026.06621264
ATGGCAA3550.025.7199870
ACGCAGA64800.024.789387
CCGAAAC951.4321768E-424.38126468
TACGGAG700.001425026924.365432
Produced by FastQC (version 0.11.5)