FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487475
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53580.36020773458377453No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCGCTGCTACTATCCCACAC50800.34151834484613186No Hit
CTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG48770.3278710566564144No Hit
CCATTACCTGTGGGGGATACAACATTGGAAGTAAACCTGTGCATTGGTATCAGCAGAAGCCAGGCCAGGCCC44910.3019210406897595No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCGCTG43030.28928217280962704No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40410.2716684314022084No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40350.2712650632783744No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC37570.25257567354073174No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35650.23966789357804333No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30830.20726398763004422No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30200.20302862232978708No Hit
GTGTTGGTCCTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG29950.20134792181381198No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29280.19684364443099886No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27340.18380140842703238No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26810.18023832333316528No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTCAGAGCACGG26640.17909544698230223No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26510.17822148271399518No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26350.17714583438377116No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26040.1750617657439621No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACT25990.17472562564076707No Hit
CTCCAGTCCCTTGCCTGGAGCCTGGCGGACCCAGGTCATGCCATAGCGAC25660.17250710095968No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25440.17102808450562196No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24440.1643052824417217No Hit
CGGCCACCCTGACCATCAGCCGGGCCGAGGGCGGGGATGAGGCCGACTAC24290.16329686213213668No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24180.16255735390510764No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23900.1606749693272156No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGC23370.15711188423334846No Hit
CCCCTGTGTTGGTCCTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT23080.15516227163481738No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22850.15361602716012032No Hit
GGGTGGCCGTGCTCTGAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT22660.1523386947679793No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA22560.15166641456158927No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG22400.15059076623136525No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22360.15032185414880922No Hit
GCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCA22020.14803610144708312No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21980.14776718936452712No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21550.14487638447705004No Hit
GTAGTAGTCGGCCTCATCCCCGCCCTCGGCCCGGCTGATGGTCAGGGTGG21440.144136876250021No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCGCTGC21400.143867964167465No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21330.14339736802299197No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21310.14326291198171398No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCAC21290.14312845594043597No Hit
ATCCCACACCTGACAGTAGTAGTCGGCCTCATCCCCGCCCTCGGCCCGGC20780.13969982688784685No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20750.13949814282592984No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20090.1350610934637557No Hit
GGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTAC20070.13492663742247768No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19600.1317669204524446No Hit
GGCCAGGCCCCTGTGTTGGTCCTCTATGATGATAGCGACCGGCCCTCAGG19500.13109464024605455No Hit
GACTACTACTGTCAGGTGTGGGATAGTAGCAGCGATCATGTGGTTTTCGG19340.13001899191583052No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19180.12894334358560647No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTA19170.12887611556496748No Hit
GGTTTACTTCCAATGTTGTATCCCCCACAGGTAATGGTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG19050.12806937931729945No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT18740.1259853106774904No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATGCACAGGTTTACTT18730.12591808265685137No Hit
GGATAGTAGCAGCGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC18640.12531303047110035No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC18550.12470797828534932No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18530.12457352224407132No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18250.12269113766617927No Hit
AAGTAAACCTGTGCATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGT17840.11993478881998015No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17720.11912805257231214No Hit
GGACTGGAGTGGGTGGCATTTATATCATATGATGGAAATGATAAATTCTA17540.1179179482008101No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17460.11738012403569807No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG17390.11690952789122507No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTACCTGTGGGGGATACAACATTGGAAGTAAACCTGTG17360.11670784382930806No Hit
GGATACAACATTGGAAGTAAACCTGTGCATTGGTATCAGCAGAAGCCAGG17130.11516159935461101No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC16950.11395149498310896No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACCATTACCTGTGGGGGATACAA16670.11206911040521689No Hit
GTGTGGGATAGTAGCAGCGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16600.11159851426074388No Hit
CTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG16590.11153128624010489No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16540.11119514613690987No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16530.11112791811627087No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16500.11092623405435387No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16310.1096489016622128No Hit
GGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATGCACAGGT16310.1096489016622128No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16200.10890939343518379No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16180.10877493739390577No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16160.10864048135262779No Hit
CTACTATCCCACACCTGACAGTAGTAGTCGGCCTCATCCCCGCCCTCGGCCCGGCTGATGGTCAGGGTGGCCGTG16070.10803542916687676No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15970.10736314896048674No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15860.1066236407334577No Hit
CTACTACTGTCAGGTGTGGGATAGTAGCAGCGATCATGTGGTTTTCGGCG15610.10494294021748264No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15600.10487571219684365No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC15600.10487571219684365No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15570.10467402813492664No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15540.10447234407300963No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15240.10245550345383957No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15190.10211936335064456No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCG15130.10171599522681055No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15130.10171599522681055No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15110.10158153918553253No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC14880.10003529471083547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)