FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487475
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCGCTGCTACTATCCCACAC49920.33560227902989964No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48230.3242407435419083No Hit
CTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG44060.2962066589354443No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41110.2763743928469386No Hit
CCATTACCTGTGGGGGATACAACATTGGAAGTAAACCTGTGCATTGGTATCAGCAGAAGCCAGGCCAGGCCC39810.26763475016386834No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCGCTG38470.258626195398242No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38460.258558967377603No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC32450.2181549269735626No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32160.21620531437503152No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30470.2048437788870401No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27320.1836669523857544No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27300.18353249634447638No Hit
GTGTTGGTCCTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG27020.1816501117665843No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26480.17801979865207818No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26260.17654078219802014No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26150.1758012739709911No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25470.17122976856753896No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACT24950.16773391149431083No Hit
GGGTGGCCGTGCTCTGAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT24940.1676666834736718No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTCAGAGCACGG24640.16564984285450177No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24150.16235566984319064No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23920.1608094253684936No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23680.15919595287315752No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGC22960.15435553538714936No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22550.15159918654095028No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22090.14850669759155616No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21910.14729659322005412No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA21460.14427133229129901No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20910.1405737911561539No Hit
CTCCAGTCCCTTGCCTGGAGCCTGGCGGACCCAGGTCATGCCATAGCGAC20860.14023765105295888No Hit
CCCCTGTGTTGGTCCTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT20560.13822081043378878No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20460.13754853022739877No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20320.13660733793845276No Hit
GCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCA20170.13559891762886772No Hit
GGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTAC20090.1350610934637557No Hit
CGGCCACCCTGACCATCAGCCGGGCCGAGGGCGGGGATGAGGCCGACTAC19800.13311148086522462No Hit
GGTTTACTTCCAATGTTGTATCCCCCACAGGTAATGGTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG19770.1329097968033076No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCAC19280.1296156237919965No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG19270.1295483957713575No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCGCTGC19230.1292794836888015No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTA18900.1270609590077144No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18890.12699373098707542No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATGCACAGGTTTACTT18620.12517857442982236No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18380.12356510193448629No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18200.12235499756298425No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17960.12074152506764821No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17790.11959864871678516No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17700.11899359653103413No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17620.11845577236592213No Hit
ATCCCACACCTGACAGTAGTAGTCGGCCTCATCCCCGCCCTCGGCCCGGC17610.1183885443452831No Hit
GGCCAGGCCCCTGTGTTGGTCCTCTATGATGATAGCGACCGGCCCTCAGG17600.11832131632464411No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT17490.11758180809761508No Hit
GACTACTACTGTCAGGTGTGGGATAGTAGCAGCGATCATGTGGTTTTCGG17480.11751458007697609No Hit
GTAGTAGTCGGCCTCATCCCCGCCCTCGGCCCGGCTGATGGTCAGGGTGG17410.11704398393250307No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17250.11596833560227904No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC17170.11543051143716701No Hit
GGATAGTAGCAGCGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC17140.11522882737525No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17070.11475823123077698No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC16830.11314475873544093No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG16710.1123380224877729No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16570.11139683019882687No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16450.11059009395115885No Hit
GTGTGGGATAGTAGCAGCGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16070.10803542916687676No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC16010.10763206104304274No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15940.10716146489856973No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15880.10675809677473572No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15850.10655641271281871No Hit
AAGTAAACCTGTGCATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGT15840.10648918469217969No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACCATTACCTGTGGGGGATACAA15760.10595136052706769No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTACCTGTGGGGGATACAACATTGGAAGTAAACCTGTG15550.10453957209364863No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15470.10400174792853663No Hit
GGATACAACATTGGAAGTAAACCTGTGCATTGGTATCAGCAGAAGCCAGG15430.1037328358459806No Hit
GGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATGCACAGGT15430.1037328358459806No Hit
CTACTATCCCACACCTGACAGTAGTAGTCGGCCTCATCCCCGCCCTCGGCCCGGCTGATGGTCAGGGTGGCCGTG15320.10299332761895157No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15310.10292609959831257No Hit
GGACTGGAGTGGGTGGCATTTATATCATATGATGGAAATGATAAATTCTA15270.10265718751575657No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCG15210.10225381939192255No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15210.10225381939192255No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15200.10218659137128355No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15090.10144708314425452No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15070.10131262710297653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)