FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093453
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGTGCTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGTTCC65150.5958189332326127No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC63890.5842958042092344No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATACCAC59800.5468913615857288No Hit
GACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATACCACAACATTGAGG55860.5108587200364351No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG55460.5072005838385372No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43880.4012975409093944No Hit
GTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGG41990.384012847374327No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41860.3828239531100102No Hit
GTAATAATTAGCCTCGTCCTCAGGCTGGAGGCCCGAAATACTCAGGGAGG39940.36526489936010054No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC39470.36096658932757053No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCAC39420.3605093223028333No Hit
AATTATTACTGTTCAACATGGGACCACAGCCTCAATGTTGTGGTATTCGG37850.34615113772608425No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37810.3457853241062945No Hit
ACCCTGTCCTGTGCTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGG34940.31953819688637736No Hit
ACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATACCACAACATTGAGGC34480.31533134025879483No Hit
GTGTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAG32920.30106460908699323No Hit
ACATGGGACCACAGCCTCAATGTTGTGGTATTCGGCGGTGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG32040.29301670945161795No Hit
GTCTGGGACCACAGCCTCCCTGAGTATTTCGGGCCTCCAGCCTGAGGACG31890.29164490837740625No Hit
GTGCTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGTTCCCAAAA31280.28606625067561203No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG30140.2756405625116031No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29350.26841574352075487No Hit
GTCCCAGACTTTGAGCCAGAGAAGCGGTCAGGGATCCCTGAGGGCAGAGA29300.2679584764960176No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28840.26375161986843515No Hit
GGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCT28750.26292853922390813No Hit
GTCCTGGGCACAGTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACCCTGTC28370.25945330983590514No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27910.2552464532083226No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27130.2481130876224218No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26970.24664983314326266No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26680.24399768439978672No Hit
GACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACCCTGT26130.23896774712767718No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25550.23366344964072533No Hit
GTCCCATGTTGAACAGTAATAATTAGCCTCGTCCTCAGGCTGGAGGCCCG25090.22945659301314278No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24220.22150014678271493No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23410.2140924209819718No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22720.20778213604059798No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22520.20595306794164908No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22290.2038496396278578No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22150.20256929195859355No Hit
GCACAGGACAGGGTGACCTTCTGTCCCACGGTCCCTGACACCGAGGCTTC20810.19031453569563575No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20620.18857692100163426No Hit
GCTCAGGGTCCTGGGCACAGTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACC20180.18455297118394665No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20020.1830897167047875No Hit
CCACAACATTGAGGCTGTGGTCCCATGTTGAACAGTAATAATTAGCCTCGTCCTCAGGCTGGAGGCCCGAAATA19430.17769396581288815No Hit
GGACAGGGTGACCTTCTGTCCCACGGTCCCTGACACCGAGGCTTCCTGGGTCAGCGCAGACTGTGCCCAGGACC19340.17687088516836114No Hit
GGCTTAGGAAGCAGAGCCTGGGGCATCTCCACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTG19280.17632216473867648No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19270.176230711333729No Hit
AGGTCACCCTGTCCTGTGCTGGAAACAGCAACAACATTGGAAGTTATGCT19190.17549908409414944No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18370.16799990488845887No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18350.16781699807856396No Hit
ATGTTGAACAGTAATAATTAGCCTCGTCCTCAGGCTGGAGGCCCGAAATA18060.16516484933508802No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATACCACAA18010.1647075823103508No Hit
GTAATATACAGCCGTTTCTTCATCTCTCAGGCCATTCATTTGCAGATACA17940.16406740847571866No Hit
TCCTCAGGCTGGAGGCCCGAAATACTCAGGGAGGCTGTGGTCCCAGACTTTGAGCCAGAGAAGCGGTCAGGGATC17940.16406740847571866No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17390.15903747120360912No Hit
GTTCAACATGGGACCACAGCCTCAATGTTGTGGTATTCGGCGGTGGGACC17310.15830584396402955No Hit
ATGTTGTGGTATTCGGCGGTGGGACCAAGCTGACCGTCCTAGGTCAGCCC17300.1582143905590821No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17200.15729985650960765No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17160.15693404288981785No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16640.15217846583255065No Hit
GGCTGGAGGCCCGAAATACTCAGGGAGGCTGTGGTCCCAGACTTTGAGCC16500.15089811816328638No Hit
CAGTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAA16320.14925195687423237No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16100.14723998196538854No Hit
GCCTGAGGACGAGGCTAATTATTACTGTTCAACATGGGACCACAGCCTCAATGTTGTGGTATTCGGCGGTGGGAC16020.14650835472580898No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16020.14650835472580898No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15970.14605108770107175No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15860.14504510024664982No Hit
CCTCCAGCCTGAGGACGAGGCTAATTATTACTGTTCAACATGGGACCACA15810.1445878332219126No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15690.14349039236254324No Hit
ACATTGAGGCTGTGGTCCCATGTTGAACAGTAATAATTAGCCTCGTCCTC15660.1432160321477009No Hit
GCCTCAATGTTGTGGTATTCGGCGGTGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA15630.14294167193285856No Hit
TCCCAAAACTGTGATGTTTGGAAATTCTCTGCCCTCAGGGATCCCTGACCGCTTCTCTGGCTCAAAGTCTGGGAC15630.14294167193285856No Hit
GTCCTGCTCAGCTGTGGGCTTAGGAAGCAGAGCCTGGGGCATCTCCACCA15530.1420271378833841No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15470.14147841745369943No Hit
GCCTGGGGCATCTCCACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCTGGGCA15330.14019806978443516No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15120.13827754828053881No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15110.13818609487559136No Hit
GAGTATTTCGGGCCTCCAGCCTGAGGACGAGGCTAATTATTACTGTTCAA15070.13782028125580156No Hit
GTATATTACTGTGCGAGATCATATTACTGTACTGGTGGTGTCTGCCAGTCCGGGCACTACTACGGTTTGGACGT14800.13535103932222053No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14790.13525958591727308No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14680.13425359846285118No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14580.1333390644133767No Hit
GTTTTGGGAACACCGTGAGAAATCTGTTGGTACCAGCCCACAGCATAACTTCCAATGTTGTTGCTGTTTCCAGCA14250.130321102050111No Hit
CCTGGGCACAGTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTG14130.12922366119074163No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14120.12913220778579418No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13850.12666296585221315No Hit
GCTTGGTCCCACCGCCGAATACCACAACATTGAGGCTGTGGTCCCATGTTGAACAGTAATAATTAGCCTCGTCCT13820.12638860563737078No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13670.1250168045631591No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13510.12355355008399996No Hit
AAGTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGTTCCCAAAACTGTGATGTTTGGAAATTCTCTGCC13490.12337064327410507No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC13470.12318773646421016No Hit
GGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAGCAACAACATTGGAAG13370.12227320241473572No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13230.12099285474547145No Hit
TTCCAGCACAGGACAGGGTGACCTTCTGTCCCACGGTCCCTGACACCGAGGCTTCCTGGGTCAGCGCAGACTGTG13110.11989541388610211No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13070.11952960026631232No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12930.11824925259704808No Hit
CTCCACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCTGGGCACAGTCTGCG12900.11797489238220572No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12600.11523129023378234No Hit
ACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCTGGGCACAGTCTGCGCTGACC12350.11294495511009618No Hit
GTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAG12210.11166460744083194No Hit
CATTGAGGCTGTGGTCCCATGTTGAACAGTAATAATTAGCCTCGTCCTCA12190.11148170063093706No Hit
GTGTTCCCAAAACTGTGATGTTTGGAAATTCTCTGCCCTCAGGGATCCCT12190.11148170063093706No Hit
GCTGTGGGCTTAGGAAGCAGAGCCTGGGGCATCTCCACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACC12080.11047571317651514No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12010.10983553934188302No Hit
GTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGTTCCCAAAACTGTGATGTTTGGAAATTCTCTGCCCT11940.1091953655072509No Hit
CCGTGGGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAGCAACAACATT11740.10736629740830195No Hit
CATTGGAAGTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGTTCCCAAAACTGTGATGTTTGGAAATT11540.10553722930935303No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11420.10443978844998367No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC11390.10416542823514134No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT11340.1037081612104041No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11250.10288508056587709No Hit
GTCCCTGACACCGAGGCTTCCTGGGTCAGCGCAGACTGTGCCCAGGACCC11200.10242781354113986No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG11060.1011474658718756No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG11050.10105601246692816No Hit
CCCTGACACCGAGGCTTCCTGGGTCAGCGCAGACTGTGCCCAGGACCCTG11040.10096455906198072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC200.00783236550.9402432
CGAGTGA400.00801596550.63915370
GGTATCA6600.043.2220231
GGCGAAT650.001041117141.5500770
CACTCGA350.001228477240.17884451
TGACGTC350.001456092138.81339324
AGGGGGG1254.7460162E-737.81056670
GTATCAA16450.036.953611
TCTGGAG6900.036.20577270
ACTCGAG400.002347249335.21651552
CCATAGA400.002805001633.9601631
GTATCGA502.0420727E-433.960161
TACTCCG850.00388454731.77358670
TGGTATC2150.031.5908492
CGTATGC553.2207737E-431.4233341
TATCAAC19150.031.3887672
ATCAACG19750.030.6071363
GTAATAG450.004996615530.18266915
TAGTCAC802.8446375E-630.07275838
TCAACGC20150.029.9995484
Produced by FastQC (version 0.11.5)