FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093453
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGTGCTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGTTCC64880.5933496912990316No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57800.5286006805962395No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATACCAC54370.49723216269926557No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG50430.4611995211499717No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45540.41647880613067045No Hit
GACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATACCACAACATTGAGG41780.3820923258704307No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40760.3727640785657911No Hit
GTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGG39510.36133240294736035No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38440.3515468886179836No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38220.34953491370913975No Hit
GTAATAATTAGCCTCGTCCTCAGGCTGGAGGCCCGAAATACTCAGGGAGG35670.326214295447541No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCAC35150.32145871839027373No Hit
ACCCTGTCCTGTGCTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGG34560.3160629674983744No Hit
AATTATTACTGTTCAACATGGGACCACAGCCTCAATGTTGTGGTATTCGG34390.3145082596142678No Hit
GTGTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAG33670.30792361445805166No Hit
GTGCTGGAAACAGCAACAACATTGGAAGTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGTTCCCAAAA33610.30737489402836704No Hit
GTCTGGGACCACAGCCTCCCTGAGTATTTCGGGCCTCCAGCCTGAGGACG32140.2939312435010924No Hit
ACATGGGACCACAGCCTCAATGTTGTGGTATTCGGCGGTGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG30670.2804875929738178No Hit
GGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCT30150.2757320159165506No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30120.27545765570170827No Hit
GTCCCAGACTTTGAGCCAGAGAAGCGGTCAGGGATCCCTGAGGGCAGAGA29110.26622086180201615No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28720.2626541790090658No Hit
ACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATACCACAACATTGAGGC28480.2604592972903271No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27790.25414901234895326No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27000.24692419335810503No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26920.24619256611852544No Hit
GTCCTGGGCACAGTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACCCTGTC26900.24600965930863056No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25700.23503525071493697No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25540.23357199623577785No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25510.2332976360209355No Hit
GTCCCATGTTGAACAGTAATAATTAGCCTCGTCCTCAGGCTGGAGGCCCG25420.2324745553764085No Hit
GACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACCCTGT25120.22973095322798512No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23270.21281207331270754No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23170.21189753926323307No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23080.21107445861870605No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23010.21043428478407394No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21630.19781371490132635No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20790.19013162888574087No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20620.18857692100163426No Hit
GCTCAGGGTCCTGGGCACAGTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACC20140.18418715756415685No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20070.18354698372952472No Hit
GGCTTAGGAAGCAGAGCCTGGGGCATCTCCACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTG20060.1834555303245773No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19100.17467600344962245No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18850.17238966832593627No Hit
GGCTGGAGGCCCGAAATACTCAGGGAGGCTGTGGTCCCAGACTTTGAGCC18370.16799990488845887No Hit
CCACAACATTGAGGCTGTGGTCCCATGTTGAACAGTAATAATTAGCCTCGTCCTCAGGCTGGAGGCCCGAAATA18240.16681101062414205No Hit
AGGTCACCCTGTCCTGTGCTGGAAACAGCAACAACATTGGAAGTTATGCT18190.16635374359940483No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17720.16205543356687485No Hit
ATGTTGAACAGTAATAATTAGCCTCGTCCTCAGGCTGGAGGCCCGAAATA17520.16022636546792593No Hit
GTAATATACAGCCGTTTCTTCATCTCTCAGGCCATTCATTTGCAGATACA17450.1595861916332938No Hit
CAGTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAA17320.158397297368977No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17210.15739130991455508No Hit
GCCTCAATGTTGTGGTATTCGGCGGTGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17070.15611096224529084No Hit
GTCCTGCTCAGCTGTGGGCTTAGGAAGCAGAGCCTGGGGCATCTCCACCA17060.1560195088403434No Hit
ATGTTGTGGTATTCGGCGGTGGGACCAAGCTGACCGTCCTAGGTCAGCCC17050.15592805543539592No Hit
GGACAGGGTGACCTTCTGTCCCACGGTCCCTGACACCGAGGCTTCCTGGGTCAGCGCAGACTGTGCCCAGGACC17000.15547078841065873No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16900.15455625436118425No Hit
GCCTGGGGCATCTCCACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCTGGGCA16800.15364172031170978No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATACCACAA16760.15327590669192No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16730.15300154647707764No Hit
GCACAGGACAGGGTGACCTTCTGTCCCACGGTCCCTGACACCGAGGCTTC16430.15025794432865427No Hit
GTTCAACATGGGACCACAGCCTCAATGTTGTGGTATTCGGCGGTGGGACC16190.14806306260991556No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16160.14778870239507322No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16130.14751434218023088No Hit
TCCTCAGGCTGGAGGCCCGAAATACTCAGGGAGGCTGTGGTCCCAGACTTTGAGCCAGAGAAGCGGTCAGGGATC16080.14705707515549366No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16070.1469656217505462No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15850.1449536468417024No Hit
GCCTGAGGACGAGGCTAATTATTACTGTTCAACATGGGACCACAGCCTCAATGTTGTGGTATTCGGCGGTGGGAC15800.14449637981696514No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15400.1408382436190673No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15130.13836900168548624No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15050.13763737444590668No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14960.13681429380137966No Hit
TCCCAAAACTGTGATGTTTGGAAATTCTCTGCCCTCAGGGATCCCTGACCGCTTCTCTGGCTCAAAGTCTGGGAC14940.13663138699148478No Hit
GAGTATTTCGGGCCTCCAGCCTGAGGACGAGGCTAATTATTACTGTTCAA14780.13516813251232565No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14760.13498522570243074No Hit
ACATTGAGGCTGTGGTCCCATGTTGAACAGTAATAATTAGCCTCGTCCTC14690.1343450518677986No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14660.1340706916529563No Hit
GACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATACCACAACATTGAGGCTGTGGTCCCATGTTGAACAGTAAT14240.13022964864516354No Hit
CCTCCAGCCTGAGGACGAGGCTAATTATTACTGTTCAACATGGGACCACA14210.1299552884303212No Hit
GTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAG14170.1295894748105314No Hit
GCTTGGTCCCACCGCCGAATACCACAACATTGAGGCTGTGGTCCCATGTTGAACAGTAATAATTAGCCTCGTCCT14160.12949802140558397No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13980.12785186011652994No Hit
GTTTTGGGAACACCGTGAGAAATCTGTTGGTACCAGCCCACAGCATAACTTCCAATGTTGTTGCTGTTTCCAGCA13810.12629715223242335No Hit
CTCCACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCTGGGCACAGTCTGCG13770.12593133861263356No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13660.12492535115821164No Hit
CCTGGGCACAGTCTGCGCTGACCCAGGAAGCCTCGGTGTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTG13630.12465099094336932No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13590.12428517732357952No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13500.12346209667905252No Hit
GCTGTGGGCTTAGGAAGCAGAGCCTGGGGCATCTCCACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACC13230.12099285474547145No Hit
AAGTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGTTCCCAAAACTGTGATGTTTGGAAATTCTCTGCC13180.12053558772073424No Hit
GTATATTACTGTGCGAGATCATATTACTGTACTGGTGGTGTCTGCCAGTCCGGGCACTACTACGGTTTGGACGT13160.12035268091083932No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC13030.11916378664652252No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12800.11706035833273125No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12790.1169689049277838No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12670.11587146406841445No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12620.11541419704367724No Hit
GTGTTCCCAAAACTGTGATGTTTGGAAATTCTCTGCCCTCAGGGATCCCT12550.1147740232090451No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12520.11449966299420276No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT12420.1135851289447283No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12290.1123962346804115No Hit
GGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAGCAACAACATTGGAAG12250.11203042106062172No Hit
ACCATGGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCTGGGCACAGTCTGCGCTGACC12240.11193896765567427No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC11890.10873809848251366No Hit
GTTATGCTGTGGGCTGGTACCAACAGATTTCTCACGGTGTTCCCAAAACTGTGATGTTTGGAAATTCTCTGCCCT11680.10681757697861727No Hit
CATTGAGGCTGTGGTCCCATGTTGAACAGTAATAATTAGCCTCGTCCTCA11640.1064517633588275No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11530.10544577590440558No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCACCGCCGAATAC11500.10517141568956323No Hit
GTCCCTGACACCGAGGCTTCCTGGGTCAGCGCAGACTGTGCCCAGGACCC11430.10453124185493112No Hit
CCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCTGGGCACAG11370.10398252142524644No Hit
CCGTGGGACAGAAGGTCACCCTGTCCTGTGCTGGAAACAGCAACAACATT11360.10389106802029899No Hit
CCCTCTGCTCAGGGTCCTGGGCACAGTCTGCGCTGACCCAGGAAGCCTCG11300.10334234759061432No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC11230.1027021737559822No Hit
TTCCAGCACAGGACAGGGTGACCTTCTGTCCCACGGTCCCTGACACCGAGGCTTCCTGGGTCAGCGCAGACTGTG11150.10197054651640264No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT11140.10187909311145518No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG10980.10041583863229604No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10980.10041583863229604No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10950.10014147841745369No Hit
GGCTGGTACCAACAGATTTCTCACGGTGTTCCCAAAACTGTGATGTTTGGAAATTCTCTGCCCTCAGGGATCC10950.10014147841745369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)