FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477875
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGCAGATCCA92140.6234627421128309No Hit
GTCTATTACTGTGCGAAAGATTTCAAAGGTAACTGGGCGATTGACTACTGGGGCCAGGGAACCCTGGTCACCGT61210.41417575911359217No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41630.28168823479658295No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39910.2700499027319631No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28660.19392709126279287No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28010.1895288843779075No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT25700.17389833375623784No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA25640.17349234542840228No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC25220.17065042713355325No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25110.16990611519918802No Hit
GTTTATTACTGCATGCAAGCGACACAATTTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA24530.16598156136344414No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24010.1624629958555358No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTCAGTAGTTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG23800.16104203670811132No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC23360.15806478897065043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23250.1573204770362852No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG23130.15650850038061406No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22960.15535820011841325No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAACTCTGTCCCTGCCCCACTGCCA22660.15332825847923537No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT22630.1531252643153176No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22250.1505540049056923No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT21990.14879472215173814No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21810.1475767571682314No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21350.14446417998815866No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20730.14026896726719107No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAGTTCCAAGAACACCTTGGATCTGCAAATGAA20480.13857734923454285No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19990.13526177789055233No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19500.1319462065465618No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATCTCTAATCGCTTC19320.13072824156305507No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19320.13072824156305507No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC19290.13052524739913726No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19050.12890129408779497No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAACTAC19010.12863063520257126No Hit
CTCCAGAGACAGTTCCAAGAACACCTTGGATCTGCAAATGAACAGCCTGAGAGTTGAGGACACGGCTGTCTATTA18860.12761566438298233No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18700.12653302884208745No Hit
GGGCTGGAGTGGGTGGGAGTGATAACAAATGGTGGAAGTAATACATACTA18360.12423242831768587No Hit
GAGTTCACACTGAAAATCAGCAGGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCAAGCGACACAATTT18330.12402943415376808No Hit
AATACATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAGTTCCAAGAACACCTTGGATCTG18240.12342045166201471No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGAGTTCACACTGAA18230.12335278694070878No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17780.12030787448194197No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT17530.11861625644929376No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17450.11807493867884633No Hit
GTATGTATTACTTCCACCATTTGTTATCACTCCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG17300.11705996785925739No Hit
CAGTAATAGACAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGCAGATC17300.11705996785925739No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17070.11550367926922102No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT16730.11320307874481941No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC16680.11286475513828977No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA16650.11266176097437199No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC16570.11212044320392456No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16500.11164679015478304No Hit
CTCCTCACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA16490.11157912543347712No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA16320.11042882517127632No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCC16260.11002283684344075No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG16160.10934618963038145No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCGCCCAGTTAC16110.10900786602385182No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16030.10846654825340438No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT15900.1075869068764273No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15890.10751924215512136No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT15840.10718091854859174No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT15790.1068425949420621No Hit
GTCTGGGACCCCAGAGAAGCGATTAGAGATCTTATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAAG15740.10650427133553245No Hit
CGTATACAGTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCCAG15730.1064366066142265No Hit
GCCTGAGAGTTGAGGACACGGCTGTCTATTACTGTGCGAAAGATTTCAAA15710.10630127717161464No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15580.10542163579463756No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15330.10373001776198935No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15160.10257971749978854No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14980.10136175251628182No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14970.10129408779497591No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14830.10034678169669288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG25200.052.77778670
TTACGAT200.007650271551.24515531
TTATATC200.00778059251.0266724
GTCGCGT200.00778267451.023228
TCGACGT3750.050.0349629
TCGTATG3050.049.81823740
ACTCGAC3700.048.80414227
GACGTCA4000.047.82881531
ATCTCGT2950.047.7785137
GACTCGA3850.046.87249826
CGTATGC3050.046.47262241
CTCGACG3900.046.3343428
ATGCCGT3150.046.2905944
GGTATCA14000.044.9581531
CGACGTC4150.044.40986330
CATCTCG3250.044.3576936
ACGAGAC4150.044.27009222
CCGTCTT3550.044.22860347
ACGTCAT4150.043.67494232
AGACTCG4150.043.4738225
Produced by FastQC (version 0.11.5)