FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477875
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGCAGATCCA82860.5606698807409287No Hit
GTCTATTACTGTGCGAAAGATTTCAAAGGTAACTGGGCGATTGACTACTGGGGCCAGGGAACCCTGGTCACCGT59860.40504102173729173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43770.29616848515605176No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40670.27519242155121376No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31270.21158758352364035No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28700.1941977501480166No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC26990.18262708280470272No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25740.17416899264146157No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT24530.16598156136344414No Hit
GTTTATTACTGCATGCAAGCGACACAATTTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA24130.16327497251120698No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTCAGTAGTTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG23820.16117736615072317No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23450.15867377146240377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23260.15738814175759114No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22660.15332825847923537No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA22570.15271927598748203No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAACTCTGTCCCTGCCCCACTGCCA22380.15143364628266937No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21650.14649412162733655No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21500.14547915080774762No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC21400.14480250359468833No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG20650.13972764949674366No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20170.13647974287405903No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACTGGC19770.13377315402182186No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19620.13275818320223293No Hit
GGGCTGGAGTGGGTGGGAGTGATAACAAATGGTGGAAGTAATACATACTA19220.13005159434999577No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19110.12930728241563055No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19040.12883362936648907No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA18820.1273450054977586No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT18750.12687135244861708No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAGTTCCAAGAACACCTTGGATCTGCAAATGAA18550.12551805802249852No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAACTAC18160.12287913389156728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17970.12159350418675463No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17820.12057853336716569No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17700.11976655671149454No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17700.11976655671149454No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC17450.11807493867884633No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17410.1178042797936226No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17390.11766895035101074No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATAAGATCTCTAATCGCTTC17220.11651865008880996No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC17150.11604499703966845No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT17110.11577433815444473No Hit
GAGTTCACACTGAAAATCAGCAGGGTGGAAGCTGAGGATGTCGGGGTTTATTACTGCATGCAAGCGACACAATTT17000.1150300262200795No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGAGTTCACACTGAA16960.11475936733485578No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16890.1142857142857143No Hit
GTATGTATTACTTCCACCATTTGTTATCACTCCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG16830.11387972595787871No Hit
CAGTAATAGACAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGCAGATC16670.11279709041698384No Hit
CTCCAGAGACAGTTCCAAGAACACCTTGGATCTGCAAATGAACAGCCTGAGAGTTGAGGACACGGCTGTCTATTA16410.11103780766302969No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16340.11056415461388819No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC16210.1096845132369111No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAGTTGGCTTCAGCAGAGGCC16120.10907553074515773No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG16060.10866954241732217No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT15580.10542163579463756No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT15570.10535397107333164No Hit
CTCCTCACCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA15460.10460965913896643No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15330.10373001776198935No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15290.10345935887676562No Hit
AATACATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAGTTCCAAGAACACCTTGGATCTG15230.10305337054893006No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCGCCCAGTTAC15190.10278271166370634No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15090.10210606445064704No Hit
GTCTGGGACCCCAGAGAAGCGATTAGAGATCTTATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAAG15020.10163241140150553No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG15000.10149708195889369No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA14870.10061744058191661No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGT14830.10034678169669288No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT14800.1001437875327751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)