FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941941
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34980.37136083894851163No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26920.2857928468980541No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26600.2823956065188796No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24760.26286147433862633No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24750.26275531057677715No Hit
GTCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCACCGTAGCAAC23460.24906018529822993No Hit
GTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTACCAACACAAGCCTGGCCAGGCTCCCAGACT23120.24545061739535703No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATT21520.22846441549948457No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTGTCCTGCAGGGCCAGTCAGAGTATTAGCAG21470.22793359669023858No Hit
GAGTATTAGCAGCTACTTAGCCTGGTACCAACACAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATC18940.20107416494239022No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18660.19810157961061256No Hit
ACACAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCGCCCCAGCCAGGTT17630.18716671214014466No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGGCGCCAGCGGCCCTGTT16980.18026606761994646No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16860.17899210247775604No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16800.1783551199066608No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16630.17655033595522437No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16440.1745332244800895No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16090.17081749281536743No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15970.16954352767317699No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15800.16773874372174052No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTGTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTA15800.16773874372174052No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15640.16604012353215328No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15520.16476615838996284No Hit
GTACCAACACAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCGCCCCAGC15140.16073193543969314No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14840.15754702258421704No Hit
GTCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTC14720.1562730574420266No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14620.1552114198235346No Hit
GACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCACCGTAGCAACTGG13850.14703681016114598No Hit
GAGCCACCCTGTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTACCAACACAAGCCTGGCCAGG13840.14693064639929676No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGACCGAACGTCCACGGAGGCCAGTTGCTACGGTG13650.14491353492416192No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCGCCCCA12720.13504030507218606No Hit
ACCCTGTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTA12580.1335540124062972No Hit
GCTCTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGGCGCCAGCGG12550.1332355211207496No Hit
GAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGGCGCCA12050.12792733302828946No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11860.1259102215531546No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11850.1258040577913054No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11800.1252732389820594No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11540.12251298117398012No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT10960.11635548298672635No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10950.11624931922487715No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10760.11423220774974228No Hit
GTTCAGTGCCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGTC10730.11391371646419467No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10710.11370138894049628No Hit
GTCTGGGAGCCTGGCCAGGCTTGTGTTGGTACCAGGCTAAGTAGCTGCTA10660.11317057013125026No Hit
GTGCCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATTTTGCAATTTATTA10580.11232126003645664No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10260.10892401965728214No Hit
GGCTTGTGTTGGTACCAGGCTAAGTAGCTGCTAATACTCTGACTGGCCCTGCAGGACAGGGTGGCTCTTTCCC10250.10881785589543294No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10240.10871169213358374No Hit
TCTATGATGCATCCAACAGGGCCGCTGGCGCCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGACTTCACT10220.10849936460988532No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC10180.10807470956248852No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10150.10775621827694092No Hit
GTATTAGCAGCTACTTAGCCTGGTACCAACACAAGCCTGGCCAGGCTCCC10050.10669458065844888No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9830.10435897789776642No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9780.10382815908852042No Hit
CCTTGGTCCCTTGACCGAACGTCCACGGAGGCCAGTTGCTACGGTGCTGA9750.1035096678029728No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC9720.10319117651742518No Hit
CTGCAGGACAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG9680.10276652147002836No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9660.10255419394632997No Hit
CAACAGGGCCGCTGGCGCCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGA9510.10096173751859192No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9490.10074940999489353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)