FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941941
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32880.349066448960179No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23820.25288208072480123No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23000.24417665225316662No Hit
GTCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCACCGTAGCAAC22490.23876230039885726No Hit
GTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTACCAACACAAGCCTGGCCAGGCTCCCAGACT22450.23833764535146043No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21200.22506717512031008No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20990.22283773612147684No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20390.21646791041052466No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTGTCCTGCAGGGCCAGTCAGAGTATTAGCAG20260.215087781506485No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATT19690.20903644708108043No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18180.19300571904185082No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17820.18918382361527952No Hit
GAGTATTAGCAGCTACTTAGCCTGGTACCAACACAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATC17750.1884406772823351No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTGTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTA16870.17909826623960526No Hit
ACACAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCGCCCCAGCCAGGTT15670.1663586148177009No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15530.16487232215181205No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGGCGCCAGCGGCCCTGTT15400.16349219324777242No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15120.16051960791599473No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14850.15765318634606626No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14660.1556360748709314No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14340.1522388344917569No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14260.1513895243969633No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14120.14990323173107445No Hit
GTACCAACACAAGCCTGGCCAGGCTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCGCCCCAGC14070.14937241292182843No Hit
GACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCACCGTAGCAACTGG13660.1450196986860111No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGACCGAACGTCCACGGAGGCCAGTTGCTACGGTG13390.14215327711608264No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13280.1409854757357414No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12830.13620810645252726No Hit
GCTCTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGGCGCCAGCGG12820.13610194269067807No Hit
GAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGGCGCCA12740.13525263259588444No Hit
GTCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTC12500.1327047023115036No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12430.13196155597855916No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12420.13185539221670997No Hit
GAGCCACCCTGTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTACCAACACAAGCCTGGCCAGG12280.13036909955082113No Hit
CTCCCAGACTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCGCCCCA12140.12888280688493228No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11850.1258040577913054No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11470.12176983484103569No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC11330.12028354217514686No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11200.11890341327110722No Hit
ACCCTGTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTACTTAGCCTGGTA11150.11837259446186119No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT10920.11593082793932953No Hit
GGCTTGTGTTGGTACCAGGCTAAGTAGCTGCTAATACTCTGACTGGCCCTGCAGGACAGGGTGGCTCTTTCCC10470.1111534586561154No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10380.11019798479947257No Hit
GTTCAGTGCCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGTC10240.10871169213358374No Hit
GTCTGGGAGCCTGGCCAGGCTTGTGTTGGTACCAGGCTAAGTAGCTGCTA10070.10690690818214729No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9950.10563294303995686No Hit
GTGCCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTAGAGCCTGAAGATTTTGCAATTTATTA9870.10478363294516323No Hit
GTATTAGCAGCTACTTAGCCTGGTACCAACACAAGCCTGGCCAGGCTCCC9790.1039343228503696No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9670.10266035770817918No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9640.10234186642263156No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9610.10202337513708397No Hit
GTGTTGGTACCAGGCTAAGTAGCTGCTAATACTCTGACTGGCCCTGCAGGACAGGGTGGCTCTTTCCCCTGGAGA9480.10064324623304433No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC9470.10053708247119511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTC150.002480615668.1045829
TCCGATA200.006315525653.78927261
CTAACGC409.326195E-750.850643
ATAAACG200.00788672750.850643
CCTAACG452.094439E-645.2173732
GGTATCA10000.040.7215841
GTATCAA25100.035.9626271
CTCGCTA601.5036121E-533.93285423
TATCAAC27850.031.6602782
TCAACGC27950.031.4123214
CCGTCAT900.004217232631.24344870
CAACGCA28850.030.4323885
AACGCAG29400.030.0936786
AGGTATC953.7338214E-728.5477263
ACGCAGA30950.028.4770347
ATCAACG31400.028.178383
CGCAGAG31200.028.1402058
ACGGGAA1353.7471182E-1027.63283716
AGAGTAC32650.027.40669811
CTAATGG1050.00895507226.78009670
Produced by FastQC (version 0.11.5)