FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891631
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33800.37908058378409903No Hit
GTAATAATCGGCCTCGTCCCCAGGCTGGAGTCCGGTGATGCCCAGGGTGG30360.34049960129246293No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26240.2942921455175964No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGC25130.2818430494229115No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23860.26759948902629005No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCCCCACTCAGG22960.25750562732789684No Hit
GATTATTACTGCGCAACATGGGATAACATCCTGAGTGGGGCGGTGTTCGG22930.25716916527128375No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22610.25358023666741064No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19530.21903679885513175No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17970.2015407719112503No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17260.1935778365714068No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16980.1904375240430178No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16520.18527843917495015No Hit
ACATGGGATAACATCCTGAGTGGGGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG15920.17854919804268807No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15890.17821273598607495No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15500.17383872925010457No Hit
GTAATATACGGCACTGTCTTCAGCCTTTAGGCTGCTGATCTGCAGATATG15490.17372657523123355No Hit
CCCAGGAACAGCCCCCAAACTCGTCATTTATGACAATAATGAACGACCCTCAGGGATTCCTGACCGATTCTCTGG15210.17058626270284458No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15020.1684553363442949No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15000.16823102830655282No Hit
ATGTTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGGCTGGAG14790.16587579391026108No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14540.1630719434384852No Hit
GGATAACATCCTGAGTGGGGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14440.16195040324977486No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14390.16138963315541968No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14330.16071670904219346No Hit
GTCCTGGGCCCAGTCTGTATTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTG14020.15723993445719137No Hit
GGACAGAGGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTCGCCTGGTACCAGCAA13580.15230515762686583No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGGCTGGAGTCCGG13310.14927699911734787No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13250.14860407500412165No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12890.14456653032476438No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12680.14221129592847265No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12510.14030467760766505No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12420.13929529143782574No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11750.13178097217346638No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCCCCACTCAGGA11550.1295378917960457No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11540.12942573777717464No Hit
GTCCTGGGGCCGCAGACACTGAGGGCGGCTGCGTCAATACAGACTGGGCC11430.12819204356959324No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTGCTCTGG11240.12606111721104357No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11200.12561250113555944No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11180.12538819309781737No Hit
GTTCCTGGGAGTTGCTGGTACCAGGCGACATAATTATTCCCAATGTTGGA10850.12168711047507322No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10730.12034126224862078No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10560.11843464392781318No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10340.1159672555126504No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10230.11473356130506902No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT10080.1130512510220035No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC9910.1111446327011959No Hit
GTACCAGCAACTCCCAGGAACAGCCCCCAAACTCGTCATTTATGACAATAATGAACGACCCTCAGGGATTCCTGA9880.1108081706445828No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTCGCCTGGTACCAGCAACTCCCAGGAACAGCCCC9730.10912586036151727No Hit
ACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAG9580.10744355007845173No Hit
GGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT9470.10620985587087034No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9390.10531262371990208No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9360.10497616166328896No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9280.10407892951232067No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9270.10396677549344964No Hit
GTATATTACTGTGTGAGAGATATACGAGATGACTACGGGTCTGATGCCTT9210.10329385138022344No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9030.10127507904054481No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG9020.10116292502167377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)