FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891631
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC31750.3560890099155368No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24420.273880114083068No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23870.2677116430451611No Hit
GTAATAATCGGCCTCGTCCCCAGGCTGGAGTCCGGTGATGCCCAGGGTGG23220.2604216318185438No Hit
GTCCTGGGCCCAGTCTGTATTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTG22390.2511128482522479No Hit
GATTATTACTGCGCAACATGGGATAACATCCTGAGTGGGGCGGTGTTCGG20880.23417759140272154No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20720.23238312710078496No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGC20640.23148589494981667No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20380.22856989045916976No Hit
GTATTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCAC19070.21387771398706418No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18610.20871862911899652No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18240.20456893042076824No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18130.20333523621318683No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16380.18370828291075567No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16090.18045581636349567No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15950.17888566009930118No Hit
CCCAGGAACAGCCCCCAAACTCGTCATTTATGACAATAATGAACGACCCTCAGGGATTCCTGACCGATTCTCTGG14940.16755810419332662No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCCCCACTCAGG14860.16666087204235833No Hit
GGATAACATCCTGAGTGGGGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14680.1646420997026797No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14620.16396917558945348No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14530.16295978941961417No Hit
GGACAGAGGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATTATGTCGCCTGGTACCAGCAA14000.1570156264194493No Hit
ACATGGGATAACATCCTGAGTGGGGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13800.15477254604202859No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13690.1535388518344472No Hit
GTAATATACGGCACTGTCTTCAGCCTTTAGGCTGCTGATCTGCAGATATG13450.15084715538154236No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13390.15017423126831617No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13250.14860407500412165No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13160.14759468883428234No Hit
ATGTTATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGGCTGGAG12960.14535160845686165No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12700.1424356039662147No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12220.13705221106040502No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGGCTGGAGTCCGG11740.13166881815459536No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11690.13110804806024018No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11600.13009866189040084No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11540.12942573777717464No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT11110.12460311496572012No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10920.12247218860717045No Hit
GGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT10870.12191141851281527No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10770.12078987832410493No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10760.1206777243052339No Hit
GTACCAGCAACTCCCAGGAACAGCCCCCAAACTCGTCATTTATGACAATAATGAACGACCCTCAGGGATTCCTGA10680.1197804921542656No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10630.11921972205991044No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10370.11630371756926351No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10360.11619156355039248No Hit
GTCCTGGGGCCGCAGACACTGAGGGCGGCTGCGTCAATACAGACTGGGCC10230.11473356130506902No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC10130.11361202111635867No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10100.11327555905974558No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10090.11316340504087453No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC10000.11215401887103521No Hit
GTTCCTGGGAGTTGCTGGTACCAGGCGACATAATTATTCCCAATGTTGGA9940.11148109475780901No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9920.11125678672006695No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9640.10811647419167794No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9580.10744355007845173No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9440.10587339381425724No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9250.10374246745570757No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTGCTCTGG9210.10329385138022344No Hit
GTATATTACTGTGTGAGAGATATACGAGATGACTACGGGTCTGATGCCTT9210.10329385138022344No Hit
GGGCATCACCGGACTCCAGCCTGGGGACGAGGCCGATTATTACTGCGCAA9140.10250877324812618No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9060.10161154109715789No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTT451.3060129E-463.27705870
TCCGGTA200.007760578851.0582333
TACGTAT700.001155306140.6781170
GTGCTAG1200.036.660251
GTGGTAT2350.034.5602381
AATACGT1004.656613E-1033.8611310
CCCTATA502.088081E-433.8307462
TGGTATC2600.033.830742
TATCAAC19500.032.6163062
GTATAAT851.4309808E-731.8496281
TCAACGC20450.031.1011264
ATCAACG20600.030.8746593
ATACGTA1101.2914825E-930.78457311
GAATACG1101.2933015E-930.7828439
CAACGCA20850.030.6718795
GTATCAA21300.030.5038721
AGAGTAC21400.030.06530411
AACGCAG21600.029.7685416
CGTACCA1152.077286E-929.4461114
CAGAGTA21800.029.35666710
Produced by FastQC (version 0.11.5)