FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333262
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACCTGGCCGACGTTCGGCCAAGGGACCAAGGTGGAAGTCGAACGAACTGTGGCTGCACCATCTGTCTT86720.650434798261707No Hit
GTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC75430.565755267906833No Hit
GTAATATATGGCGGTGTCCTCAGTTGTCAGACTGTTCATTTGCAGATACA47250.35439396007686413No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42960.3222172386222663No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38220.28666533659550786No Hit
GTATGCAGACTCTGTGCAGGGCCGATTCACCATCTCCAGAGACAACAACTTAAACTCCCTGTATCTGCAAATGAA32890.24668819781858328No Hit
ATATATTACTGTGTAAAAGCAGCTCTTCGGGCCGACTACTACTACTACTG32230.24173793297941443No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCAACCA30790.23093735514850044No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27900.20926119547395786No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25410.1905851963080025No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24060.18045965459152064No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT23390.17543438573963707No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23380.1753593817269224No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22360.167708972430025No Hit
CTCCTGTGCAGCCTCTGGATTTAGATTTGACGATTATTCCATGCACTGGGTCCGTCAAACTCCGGGGAAGGGT22170.16628389618844608No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCACTGTGAAGTGCAGCTGGTGGAGT21910.1643337918578644No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21680.16260869956542676No Hit
GTCCCAGGCTCCTCATCTATGATTCATCCACCAGGGCCACTGGTGTCCCA21520.16140863536199188No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21510.1613336313492772No Hit
GTGCAGGGCCGATTCACCATCTCCAGAGACAACAACTTAAACTCCCTGTATCTGCAAATGAACAGTCTGACAA21370.16028357517127165No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21360.160208571158557No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21260.1594585310314102No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGATGGAGCCATCGTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG20950.15713340663725509No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATACCTGG20900.15675838657368169No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20860.156458370522823No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20700.15525830631938808No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20140.151058081607366No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20060.15045804950564856No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19800.14850794517506685No Hit
GTCTGTGTCTCCAGGGGATGGAGCCATCGTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAAGTTAGCCTGGTA18690.14018249976373737No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18250.13688232320429145No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT18160.1362072870898593No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18060.1354572469627125No Hit
GTCTGCATACTTTGTGCGAGCACCATCCCAGTTAGTAACCGAGACCCATT17860.1339571667084189No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16940.1270567975386683No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC16910.12683178550052426No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16870.12653176944966557No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16820.12615674938609214No Hit
CAGTAATATATGGCGGTGTCCTCAGTTGTCAGACTGTTCATTTGCAGATA16620.12465666913179854No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16580.12435665308093984No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15820.11865634811462414No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15730.11798131200019202No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15660.11745628391118924No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15470.11603120766961032No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15390.11543117556789288No Hit
GTTTATTACTGTCAGCAGTATAATACCTGGCCGACGTTCGGCCAAGGGAC15130.11348107123731119No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15000.11250601907202035No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14820.11115594684315612No Hit
GCCCTGCACAGAGTCTGCATACTTTGTGCGAGCACCATCCCAGTTAGTAACCGAGACCCATTCCAGACCCTTCCC14660.10995588263972123No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14230.10673071009298998No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14160.10620568200398722No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG14140.10605567397855785No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14090.10568065391498445No Hit
GATCAGCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGG13990.10493061378783765No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13910.10433058168612022No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13830.10373054958440275No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13700.10275549741911193No Hit
GTGTTAACAGCAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGATTCATCCA13630.10223046933010915No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13450.10088039710124493No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13420.10065538506310089No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13390.10043037302495683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)