FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333262
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACCTGGCCGACGTTCGGCCAAGGGACCAAGGTGGAAGTCGAACGAACTGTGGCTGCACCATCTGTCTT87240.6543350069228704No Hit
GTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC76590.574455733381736No Hit
GTAATATATGGCGGTGTCCTCAGTTGTCAGACTGTTCATTTGCAGATACA45960.3447184424366703No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43120.3234173028257012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36860.2764647908663113No Hit
GTATGCAGACTCTGTGCAGGGCCGATTCACCATCTCCAGAGACAACAACTTAAACTCCCTGTATCTGCAAATGAA32450.24338802125913736No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30760.23071234311035643No Hit
ATATATTACTGTGTAAAAGCAGCTCTTCGGGCCGACTACTACTACTACTG30570.22928726686877748No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCAACCA30220.22666212642376368No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28340.21256137203340378No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT23360.17520937370149303No Hit
CTCCTGTGCAGCCTCTGGATTTAGATTTGACGATTATTCCATGCACTGGGTCCGTCAAACTCCGGGGAAGGGT23330.174984361663349No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23210.17408431351077283No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22650.16988408879875075No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCACTGTGAAGTGCAGCTGGTGGAGT22300.16725894835373692No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22180.16635890020116076No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATACCTGG20990.1574334226881138No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20760.1557083303956762No Hit
GTCCCAGGCTCCTCATCTATGATTCATCCACCAGGGCCACTGGTGTCCCA20680.15510829829395872No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20680.15510829829395872No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20600.15450826619224128No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20470.15353321402695044No Hit
GTGCAGGGCCGATTCACCATCTCCAGAGACAACAACTTAAACTCCCTGTATCTGCAAATGAACAGTCTGACAA20110.15083306956922196No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19430.1457327967046237No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19420.145657792691909No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGATGGAGCCATCGTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG18740.14055751982731077No Hit
GTCTGCATACTTTGTGCGAGCACCATCCCAGTTAGTAACCGAGACCCATT18210.1365823071534327No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18100.13575726301357122No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17630.13223207441598125No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17600.13200706237783721No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17490.13118201823797573No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17440.13080699817440233No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT17410.1305819861362583No Hit
GTCTGTGTCTCCAGGGGATGGAGCCATCGTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAAGTTAGCCTGGTA17180.12885689384382065No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17040.1278068376658151No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16990.1274318176022417No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16820.12615674938609214No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16500.12375662097922238No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16480.12360661295379304No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16320.12240654875035814No Hit
CAGTAATATATGGCGGTGTCCTCAGTTGTCAGACTGTTCATTTGCAGATA16230.12173151263592602No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16020.12015642836891774No Hit
GTTTATTACTGTCAGCAGTATAATACCTGGCCGACGTTCGGCCAAGGGAC15760.11820632403833604No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14910.11183098295758823No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14730.11048091072872399No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC14600.10950585856343314No Hit
GTGTTAACAGCAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGATTCATCCA14600.10950585856343314No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14420.10815578633456889No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14100.10575565792769913No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14060.10545564187684042No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13990.10493061378783765No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13610.10208046130467979No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA13420.10065538506310089No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13410.10058038105038619No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13390.10043037302495683No Hit
GATCAGCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGG13380.10035536901224215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT851.1492793E-649.3296970
CCTAACG306.9744245E-445.0773662
CTAACGC405.642639E-542.253693
GGTATCA14550.040.4590531
TTCGGCC21200.034.9518620
TCTGGAG17600.034.94186470
CGGCCAA21200.034.8053322
CGTTCGG21200.034.7870418
GTATCAA33650.034.4856571
TAACGGC501.955014E-434.213636
GACGTTC21650.034.2112516
ACGTTCG21850.033.90319417
GTCGTAC400.00285764233.8321381
CGACGTT21750.033.734115
CCGACGT21950.033.41544714
CGAACGA22550.033.4115246
GAACGAA22800.033.09255247
GTTCGGC22300.033.07232719
GTCGAAC22550.032.97071544
AAGGGAC22200.032.84303727
Produced by FastQC (version 0.11.5)