FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638771
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA43660.2664191641175003No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29200.17818230857148437No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT28730.1753143056595461No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28460.17366672951864537No Hit
GTACACAATTTCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA26310.16054714172999154No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24490.14944125811354972No Hit
GTGTATTACTGTGCGAGACATGAGAGAAGTGGATACACTTTTGATCTTAA23900.1458409991389889No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22290.13601656363213652No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21230.12954830174563742No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20670.12613110678673226No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19040.11618462860277609No Hit
GTTTATTACTGCATGCAAGGTACACAATTTCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA18990.1158795219100167No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18980.11581850057146484No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGA18970.11575747923291296No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18520.11301151899807844No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATTTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA18220.11118087884152208No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18030.1100214734090364No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA17590.10733653451275377No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17530.1069704064814425No Hit
GTCTCTGCTGGCTCCATCAGCAGGAGGACTGAGTACTGGGGATGGATCCG17410.10623815041881995No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16930.1033091261683298No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16870.10294299813701853No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16830.10269891278281101No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16480.10056316593349529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6300.043.265641
TCGTATG6400.042.46650340
ATGCCGT6600.041.63604444
GGTATCA17600.040.264141
ATCTCGT5400.038.50733637
ACGAGAC7100.038.1371722
TATGCCG7200.038.05880743
CGAGACT7050.037.94505723
TAGGCAT7350.037.01394729
GACTAGG7150.037.0110826
GTGGTAT5350.036.863241
AGACTAG7200.036.73159425
GAGACTA7200.036.70242724
CCGTCTT7550.036.6965747
CCTAACG1550.036.661692
ATCTCCG7750.035.79360210
GTATCAA41900.035.7403871
CTTATAC7650.035.393121
TGGTATC5700.035.186142
CACGAGA7700.035.15921
Produced by FastQC (version 0.11.5)