FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092060
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC46340.4243356592128638No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGGGATCACTACTACTATCCCACACCTGA42750.39146200758200095No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG42720.3911872974012417No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGGGATCACTACTACTATCCCACACCTGACAGTGATAGT38470.35227002179367434No Hit
CCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTATCAGCAGAAGCCTGGCCAGGCCC35760.3274545354650843No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33340.3052945808838342No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31910.29220006226764095No Hit
GGGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTAC29640.27141365859018735No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28160.2578612896727286No Hit
GTAGTAGTGATCCCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA27970.25612145852791973No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27110.24824643334615315No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26490.24256908961046098No Hit
CTACTATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCATCGAC25160.23039027159679873No Hit
GTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTAC24980.2287420105122429No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24390.22333937695731004No Hit
CACTACTACTATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCATCGACCCTGCTGATGGTCAGGGTGG24350.22297309671629764No Hit
CTATTATCATAGACGACAAGCACAGGGGCCTGGCCAGGCTTCTGCTGATA24250.22205739611376665No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23330.21363295057048146No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23050.21106898888339468No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22630.2072230463527645No Hit
GCACAGGGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA22550.2064904858707397No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG22500.2060326355694742No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACCTCCTATGTGCTGACT22350.20465908466567773No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22060.2020035529183378No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21700.19870703074922622No Hit
GGATAGTAGTAGTGATCCCCTATTCGGCGGAGGGACCAAGCTGACCGTCC21610.19788290020694835No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20770.19019101514568798No Hit
CTACTACTATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCATC20740.18991630496492864No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20370.18652821273556397No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGA20290.1857956522535392No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19960.1827738402651869No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19850.18176656960240278No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTG19530.1788363276743036No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACCTCCTATGTGCTGACTCAGC19220.17599765580645751No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGGGATCACTACTAC18760.17178543303481494No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18740.17160229291430873No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18460.16903833122722195No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18420.16867205098620955No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18380.16830577074519715No Hit
CGGCCACCCTGACCATCAGCAGGGTCGATGCCGGGGATGAGGCCGACTAT18070.16546709887735106No Hit
CCTCTCTCACTGCACAGGGTCTGTGACCTCCTATGTGCTGACTCAGCCAC17780.16281156713001116No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17630.16143801622621468No Hit
TAGTAGTAGTGATCCCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT17470.15997289526216507No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17400.1593319048403934No Hit
ATGATAATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGA17360.158965624599381No Hit
GCTGATACCAGTGCACACTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATGGTGGCCGTCTTTCCTGGGGCCA17290.15832463417760928No Hit
CTCACTGCACAGGGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG17230.1577752138160907No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATTATCATAGA17030.1559438126110287No Hit
CCCCTGTGCTTGTCGTCTATGATAATAGCGACCGGCCCTCAGGGATCCCT16360.14980861857407102No Hit
ATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCATCGACCCTGC16250.14880134791128694No Hit
TGATAATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC16190.14825192754976832No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACCTCCTA15840.14504697544090983No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15690.14367342453711335No Hit
GCACAGGGGCCTGGCCAGGCTTCTGCTGATACCAGTGCACACTTTTACTT15690.14367342453711335No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15650.14330714429610095No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG15550.14239144369356996No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15480.14175045327179825No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15380.14083475266926726No Hit
GGTATCAGCAGAAGCCTGGCCAGGCCCCTGTGCTTGTCGTCTATGATAATAGCGACCGGCCCTCAGGGATCC15020.13753823050015568No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC14890.13634781971686538No Hit
CTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATGGTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG14840.1358899694155999No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14830.13579839935534677No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14390.13176931670421038No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14130.1293884951376298No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14090.1290222148966174No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTG13990.1281065142940864No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13850.126824533450543No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG13800.12636668314927751No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAC13720.1256341226672527No Hit
GTGATAGTCGGCCTCATCCCCGGCATCGACCCTGCTGATGGTCAGGGTGG13690.1253594124864934No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13680.1252678424262403No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13500.12361958134168452No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13490.12352801128143141No Hit
GGCCAGGCCCCTGTGCTTGTCGTCTATGATAATAGCGACCGGCCCTCAGG13360.12233760049814113No Hit
GCAGAAGCCTGGCCAGGCCCCTGTGCTTGTCGTCTATGATAATAGCGACCGGCCCTCAGGGATCCCTGAGCGATT13200.12087247953409153No Hit
ACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAA13190.12078090947383842No Hit
CCTGAGGGCCGGTCGCTATTATCATAGACGACAAGCACAGGGGCCTGGCCAGGCTTCTGCTGATACCAGTGCACA13150.12041462923282603No Hit
GAAATACACGGCCGTGTCCGCAGCGGTCACAGAACTCAACTTCAGGGAGA13030.11931578850978884No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13020.11922421844953573No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12870.11785066754573925No Hit
GGTATCAGCAGAAGCCTGGCCAGGCCCCTGTGCTTGTCGTCTATGATAAT12680.11611083640093035No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12590.11528670585865247No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12570.11510356573814626No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12480.11427943519586838No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12410.11363844477409665No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12410.11363844477409665No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12300.11263117411131256No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12300.11263117411131256No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACCATTACCTGTGGGGGAAACAA12260.11226489387030016No Hit
GGCTTATAGCAATTCGGGCAAGTTCTTCGGGATGGACGTCTGGGGCCAAG12150.11125762320751606No Hit
CAGCAGGGTCGATGCCGGGGATGAGGCCGACTATCACTGTCAGGTGTGGGATAGTAGTAGTGATCCCCTATTCGG11950.10942622200245408No Hit
GACTATCACTGTCAGGTGTGGGATAGTAGTAGTGATCCCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT11800.10805267109865759No Hit
AGCCTGGCCAGGCCCCTGTGCTTGTCGTCTATGATAATAGCGACCGGCCC11730.10741168067688589No Hit
GCTTGGTCCCTCCGCCGAATAGGGGATCACTACTACTATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGG11690.10704540043587349No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGGGATCACTA11620.1064044100141018No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11520.10548870941157079No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC11450.1048477189897991No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11310.1035657381462557No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT11310.1035657381462557No Hit
CAGGTAATGGTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTCACAGAC11270.1031994579052433No Hit
CTGCACAGGGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAC11260.1031078878449902No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11240.10292474772448401No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11210.10265003754372472No Hit
GAATAGGGGATCACTACTACTATCCCACACCTGACAGTGATAGTCGGCCT11200.10255846748347161No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11200.10255846748347161No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACCTC11140.10200904712195302No Hit
GGCTTCTGCTGATACCAGTGCACACTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATGGTGGCCGTCTTTC11100.10164276688094062No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11040.101093346519422No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11020.10091020639891582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGC150.002140591270.6847150
CTAGATT150.002294209869.46023644
AGGGGGG2450.055.69116670
TTCGTCC200.00817153350.396843
CGTATGC2400.045.98038541
TATCTCG2300.045.97030636
TCTCGTA2400.045.65212238
CTTATAC2600.045.2279361
TCGTATG2550.044.5254940
TTATACA2600.043.9357072
ACGAGAC2650.043.12252822
CGGACTC2600.042.7904328
ATGCCGT2600.042.74476644
CTTGAAA2800.042.53860557
CGAGACG2700.042.325923
ATACACA2800.041.9973684
TATACAC2750.041.5392153
CGTCTTC2800.041.35273748
ACTCCTA2700.041.34807631
ATCTCGT2650.041.27021837
Produced by FastQC (version 0.11.5)