FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092060
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGGGATCACTACTACTATCCCACACCTGA43450.3978719117997179No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC42870.3925608483050381No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG39440.36115231763822503No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGGGATCACTACTACTATCCCACACCTGACAGTGATAGT37640.34466970679266706No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36400.3333150193212827No Hit
CCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTATCAGCAGAAGCCTGGCCAGGCCC33200.30401260004029085No Hit
GGGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTAC32110.2940314634727029No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28350.2596011208175375No Hit
GTAGTAGTGATCCCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA27300.24998626449096203No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26680.24430892075526983No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26440.24211123930919548No Hit
GTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTAC26430.2420196692489424No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24420.22361408713806935No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23810.21802831346263027No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACCTCCTATGTGCTGACT23650.21656319249858066No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22950.21015328828086371No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22860.2093291577385858No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22770.2085050271963079No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22610.2070399062322583No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22550.2064904858707397No Hit
CACTACTACTATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCATCGACCCTGCTGATGGTCAGGGTGG22480.20584949544896802No Hit
CTACTATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCATCGAC22420.20530007508744944No Hit
CTATTATCATAGACGACAAGCACAGGGGCCTGGCCAGGCTTCTGCTGATA22360.2047506547259308No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATTATCATAGA22120.20255297327985644No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG21760.19925645111074486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21470.19660091936340493No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21420.19614306906213944No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTG21180.19394538761606506No Hit
GGTATCAGCAGAAGCCTGGCCAGGCCCCTGTGCTTGTCGTCTATGATAAT21040.19266340677252164No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20780.19028258520594105No Hit
GCACAGGGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA20650.18909217442265078No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20620.18881746424189147No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGA20150.18451367140999578No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG19940.1825907001446807No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19630.1797520282768346No Hit
GGATAGTAGTAGTGATCCCCTATTCGGCGGAGGGACCAAGCTGACCGTCC19420.17782905701151952No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACCTCCTATGTGCTGACTCAGC19390.1775543468307602No Hit
CTACTACTATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCATC18650.17077816237203086No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18460.16903833122722195No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18060.16537552881709794No Hit
CCTCTCTCACTGCACAGGGTCTGTGACCTCCTATGTGCTGACTCAGCCAC17340.1587824844788748No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16900.15475340182773842No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16840.1542039814662198No Hit
ATGATAATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGA16830.1541124114059667No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGGGATCACTACTAC16440.15054117905609582No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16280.14907605809204622No Hit
TAGTAGTAGTGATCCCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT16040.14687837664597184No Hit
CGGCCACCCTGACCATCAGCAGGGTCGATGCCGGGGATGAGGCCGACTAT15930.14587110598318775No Hit
GCTGATACCAGTGCACACTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATGGTGGCCGTCTTTCCTGGGGCCA15920.14577953592293463No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC15890.14550482574217535No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACCTCCTA15850.14513854550116295No Hit
CCCCTGTGCTTGTCGTCTATGATAATAGCGACCGGCCCTCAGGGATCCCT15660.14339871435635404No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15560.14248301375382305No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15420.14120103291027963No Hit
CTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATGGTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG15080.13808765086167427No Hit
GCACAGGGGCCTGGCCAGGCTTCTGCTGATACCAGTGCACACTTTTACTT15030.13762980056040877No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACCTC15000.13735509037964946No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14900.13643938977711847No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14840.1358899694155999No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG14790.13543211911433437No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14580.1335091478490193No Hit
CTCACTGCACAGGGTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG14540.1331428676080069No Hit
ATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCATCGACCCTGC14420.1320440268849697No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAC14420.1320440268849697No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT14390.13176931670421038No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14350.13140303646319798No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14260.1305789059209201No Hit
TGATAATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC14070.1288390747761112No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13990.1281065142940864No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13830.1266413933300368No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13790.1262751130890244No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13710.12554255260699962No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13380.12252074061864732No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13370.12242917055839424No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTG13350.12224603043788802No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13300.12178818013662251No Hit
GGCCAGGCCCCTGTGCTTGTCGTCTATGATAATAGCGACCGGCCCTCAGG13110.12004834899181364No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12960.11867479808801715No Hit
GGCTTATAGCAATTCGGGCAAGTTCTTCGGGATGGACGTCTGGGGCCAAG12950.11858322802776404No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12700.11629397652143655No Hit
GCAGAAGCCTGGCCAGGCCCCTGTGCTTGTCGTCTATGATAATAGCGACCGGCCCTCAGGGATCCCTGAGCGATT12640.11574455615991795No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12510.11455414537662767No Hit
ACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAA12490.11437100525612146No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACCATTACCTGTGGGGGAAACAA12290.11253960405105948No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12210.11180704356903468No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12120.11098291302675677No Hit
CCTGAGGGCCGGTCGCTATTATCATAGACGACAAGCACAGGGGCCTGGCCAGGCTTCTGCTGATACCAGTGCACA11780.10786953097815137No Hit
GCTTGGTCCCTCCGCCGAATAGGGGATCACTACTACTATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGG11720.10732011061663278No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11670.10686226031536727No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11670.10686226031536727No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGGGGATCACTA11660.1067706902551142No Hit
GTGATAGTCGGCCTCATCCCCGGCATCGACCCTGCTGATGGTCAGGGTGG11640.10658755013460798No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11570.10594655971283629No Hit
GGCTTCTGCTGATACCAGTGCACACTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATGGTGGCCGTCTTTC11500.10530556929106459No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT11480.10512242917055839No Hit
GTGTGGGATAGTAGTAGTGATCCCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC11430.1046645788692929No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11400.1043898686885336No Hit
GAAATACACGGCCGTGTCCGCAGCGGTCACAGAACTCAACTTCAGGGAGA11390.1042982986282805No Hit
GACTATCACTGTCAGGTGTGGGATAGTAGTAGTGATCCCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT11330.1037488782667619No Hit
CAGCAGGGTCGATGCCGGGGATGAGGCCGACTATCACTGTCAGGTGTGGGATAGTAGTAGTGATCCCCTATTCGG11210.10265003754372472No Hit
GAATAGGGGATCACTACTACTATCCCACACCTGACAGTGATAGTCGGCCT10940.10017764591689102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)