FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004688067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671738
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA70380.4209989842906005No Hit
GTATTATTGTCATCAGAGTCATAGTTCACCGTGGACGTTCGGCCAAGGGA53650.3209234939924797No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48840.292151042806947No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG42480.2541068038173446No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41750.24974009085155685No Hit
GTCTATTACTGTGCGAGACATGGTATGATAGATGGTCTTGATGTCTGGGG41070.2456724678149327No Hit
CAATAATACGTTGCAGCATCTTCAGCTTCCAGGCTCTTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA34950.2090638604853153No Hit
AGGTTACACTGGTACCAGCAGAAACCATATCAGTCTCCAAAGCTCCTCAT34920.20888440652781717No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34160.20433823960453132No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA33230.19877516692208946No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32000.19141755466466634No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30860.1845983042797376No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30660.18340194456308345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27840.16653327255825973No Hit
GAGTCATAGTTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26580.1589962063433385No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT26410.15797930058418244No Hit
GATTTCACCCTCACCATCAAGAGCCTGGAAGCTGAAGATGCTGCAACGTATTATTGTCATCAGAGTCATAGTTCA25600.15313404373173306No Hit
GCATAAAGACAGATGGAAGTGTAAAGGACTACGTGGACTCTTTGAGGGGC24680.14763078903512392No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24600.14715224514846226No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24020.14368280197016517No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23800.14236680628184561No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22910.13704300554273458No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22700.1357868278402477No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22550.13488955805275707No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22230.1329753825061104No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22190.1327361105627796No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22120.13231738466195062No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21840.1306424810586348No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21770.13022375515780582No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21310.12747212780950123No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAAGAGCCTGGAAG21310.12747212780950123No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20760.1241821385887023No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20680.12370359470204063No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20500.12262687095705188No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGTTACTGAAGATGAATCCAGAGACTTCACA19810.11849942993459502No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT19200.11485053279879981No Hit
GTGTAACCTAATATCAATGTTCTGACTGGCCCGGCAGGTGATGGTGACTT19190.1147907148129671No Hit
CTATCATACCATGTCTCGCACAGTAATAGACAGCCGTGTCCTCGGCTCTC19080.11413271696880732No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18950.1133550831529821No Hit
GTATGATAGATGGTCTTGATGTCTGGGGCCAAGGGACAATGGTCATCGTCTCTTCAGGGAGTGCATCCGCCCCAA18550.11096236371967377No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG18460.1104240018471794No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATA18180.10874909824386356No Hit
GTCCATCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAAGAGC18160.10862946227219816No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18020.10779201047054024No Hit
CTATGACTCTGATGACAATAATACGTTGCAGCATCTTCAGCTTCCAGGCTCTTGATGGTGAGGGTGAAATCTGTC18010.10773219248470753No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC17840.10671528672555149No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT17770.10629656082472252No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT17590.10521983707973377No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17410.10414311333474505No Hit
CTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG17320.10360475146225068No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17210.10294675361809087No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG16980.10157093994393859No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16960.10145130397227317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)