FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671738
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA56990.34090270126060424No Hit
GTATTATTGTCATCAGAGTCATAGTTCACCGTGGACGTTCGGCCAAGGGA48090.2876646938694939No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47870.2863486981811743No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG44090.2637374995364106No Hit
GTCTATTACTGTGCGAGACATGGTATGATAGATGGTCTTGATGTCTGGGG38520.23041888142759215No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35040.20960222235780965No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA33720.20170624822789218No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32870.19662171943211196No Hit
AGGTTACACTGGTACCAGCAGAAACCATATCAGTCTCCAAAGCTCCTCAT32510.19446827194213445No Hit
CAATAATACGTTGCAGCATCTTCAGCTTCCAGGCTCTTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA32300.1932120942396476No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29440.17610415029149304No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28570.17089998552404742No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27340.16354237326662432No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26330.15750075669752078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25720.1538518595617256No Hit
GAGTCATAGTTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT25380.1518180480434135No Hit
GATTTCACCCTCACCATCAAGAGCCTGGAAGCTGAAGATGCTGCAACGTATTATTGTCATCAGAGTCATAGTTCA24360.14571661348847725No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23540.1408115386501952No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22840.1366242796419056No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22200.1327959285486123No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21860.13076211703030022No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21820.13052284508696937No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21610.1292666673844825No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21420.12813012565366105No Hit
GCATAAAGACAGATGGAAGTGTAAAGGACTACGTGGACTCTTTGAGGGGC21290.1273524918378358No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21190.12675431197950876No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20490.12256705297121917No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20370.12184923714122667No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20340.12166978318372855No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20310.12149032922623042No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20110.12029396950957626No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20010.11969578965124919No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGTTACTGAAGATGAATCCAGAGACTTCACA19900.11903779180708939No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19250.11514962272796336No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAAGAGCCTGGAAG18660.11162036156383358No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18060.10803128241387108No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17470.1045020212497413No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17460.10444220326390859No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG17450.10438238527807588No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17450.10438238527807588No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17370.1039038413914142No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17290.10342529750475254No Hit
CTATCATACCATGTCTCGCACAGTAATAGACAGCCGTGTCCTCGGCTCTC17260.10324584354725441No Hit
GTCCATCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAAGAGC17230.1030663895897563No Hit
GTGTAACCTAATATCAATGTTCTGACTGGCCCGGCAGGTGATGGTGACTT17080.10216911980226566No Hit
GTATGATAGATGGTCTTGATGTCTGGGGCCAAGGGACAATGGTCATCGTCTCTTCAGGGAGTGCATCCGCCCCAA16970.10151112195810588No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16900.10109239605727693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA200.00670144252.9906255
CGATAGA200.007429969551.62558737
GGTATCA14500.039.4511871
GTATCAA39150.038.0074271
GACCGGA400.002347244935.21765553
TATCAAC42050.035.1222652
ATCAACG42450.034.776743
TCAACGC42700.034.4872784
CAACGCA43600.033.8533865
AACGCAG44200.033.6246726
GTGGTAT5250.033.077271
ACGCAGA46750.031.7905987
CGCAGAG47550.031.3272638
CGCCCAA450.00420944431.26004252
GACAGGT2950.031.1645281
AGAGTAC48350.030.4563111
TGGTATC5500.030.3174132
CAGAGTA48700.030.30816510
TCTGGAG23700.030.28782370
ATAGGTG14650.030.1789844
Produced by FastQC (version 0.11.5)