FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770508
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28150.365343383845463No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20160.2616455637060225No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCTGCCGAAGACCACAGCGCCGCTGTCAGGGTCCCACGA19110.24801819059633384No Hit
CCTCTGAACTGACTCAGCCGCTCTCAGTGTCCGTGTCTCCAGGACAGACA18890.24516293146858958No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18690.24256724135245838No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17790.23088663582986807No Hit
GTCTATTACTGTGCGAAACACACCGGTAGAAGTGGTTATGAAAATGATGC17550.22777180769051067No Hit
GTAATAGACGGCCGTGTCCGCAGTGGTCATAGAGGTCACTTTCAGGGACAGTTCCTTCTTGCCCCAATCAAAAGA17130.2223208584466352No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCTGCCGAAGACCACAGCGCCGCTG17040.22115279789437617No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16560.21492314161566137No Hit
GGATGTGTGGCCTCCTCTGAACTGACTCAGCCGCTCTCAGTGTCCGTGTCTCCAGGACAGACAGCCATCATCAC16430.2132359430401761No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15810.20518930368016944No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15290.19844050937822838No Hit
CTCCAGGACAGACAGCCATCATCACCTGCTCTGGAACTATTTTGCGGAGT14670.19039387001822175No Hit
ACCCAGGCTATGGACGAGTCTGACTTTTACTGTCAGTCGTGGGACCCTGA14600.18948537847757585No Hit
GGCTATGGACGAGTCTGACTTTTACTGTCAGTCGTGGGACCCTGACAGCGGCGCTGTGGTCTTCGGCAGGGGGAC14570.18909602496015615No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCC14020.18195787714079542No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13600.17650692789691996No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13120.17027727161820513No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12610.16365826182207063No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12570.16313912379884438No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11900.15444356190980496No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11750.15249679432270657No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11670.15145851827625412No Hit
CTCTTACCATCAGCGCGACCCAGGCTATGGACGAGTCTGACTTTTACTGTCAGTCGTGGGACCCTGACAGCGGCG11410.14808412112528357No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11400.14795433661947702No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11110.1441905859510868No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11100.14406080144528025No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10820.14042683528269662No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10710.13899920571882446No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10600.1375715761549523No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10560.1370524381317261No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10150.13173127339365717No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10060.13056321284139813No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10030.13017385932397849No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10000.1297845058065588No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9920.12874622976010633No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9670.12550161711494234No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9370.12160808194074557No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9220.1196613143536472No Hit
GCACAGGATGTGTGGCCTCCTCTGAACTGACTCAGCCGCTCTCAGTGTCC9180.11914217633042098No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9120.11836346929558163No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8860.1149890721446111No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8750.11356144258073894No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8560.11109553697041433No Hit
TCCATAGCCTGGGTCGCGCTGATGGTAAGAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAAGCGCTCAGAGATC8370.1086296313600897No Hit
AGCCTGGGTCGCGCTGATGGTAAGAGTGGCTGTGTTCCCAGACTTGGAGC8360.10849984685428316No Hit
CTTACCATCAGCGCGACCCAGGCTATGGACGAGTCTGACTTTTACTGTCAGTCGTGGGACCCTGACAGCGGCG8270.10733178630202413No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8070.10473609618589295No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG8000.10382760464524703No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8000.10382760464524703No Hit
TGGTAAGAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAAGCGCTCAGAGATCCCTGAGGGCCGCCTGGTATCTT7990.10369782013944047No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7970.10343825112782735No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCTGCCGAAGACCACAGCGCCGCTGT7960.1033084666220208No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7880.10227019057556834No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7840.1017510525523421No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7830.10162126804653554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGG200.00796448850.72369818
GTGGTAT2200.039.961531
GGGACAA7850.035.00035570
GAGCCAT6300.032.70866870
AATGGTA2605.456968E-1231.70224470
CGAACTA652.6586127E-531.21863424
GGAATAA1353.0933712E-530.52809170
AGTAAAA9700.030.44940870
ATCAACG24800.029.8595923
TCAACGC24950.029.6800774
TCTCGTA1052.4681867E-829.39516838
ATGCCGT1301.8189894E-1029.38974244
AACGCAG25400.029.1542476
CAACGCA25600.028.9264815
CAAAAGA6750.028.78823769
CGAGACA1302.4920155E-1028.6115123
GTAATAG5450.028.5399231
TCGTATA1800.028.22274861
GGGAATA1104.814239E-628.10421669
GAGTTTA3150.027.9096411
Produced by FastQC (version 0.11.5)