FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004688070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770508
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26720.3467841995151251No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCTGCCGAAGACCACAGCGCCGCTGTCAGGGTCCCACGA21400.2777388424260358No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18310.23763543013180913No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17890.23218448088793367No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCTGCCGAAGACCACAGCGCCGCTG17890.23218448088793367No Hit
CCTCTGAACTGACTCAGCCGCTCTCAGTGTCCGTGTCTCCAGGACAGACA17690.22958879077180247No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17210.2233591344930877No Hit
GGATGTGTGGCCTCCTCTGAACTGACTCAGCCGCTCTCAGTGTCCGTGTCTCCAGGACAGACAGCCATCATCAC16870.21894646129566467No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16260.21102960644146457No Hit
GTCTATTACTGTGCGAAACACACCGGTAGAAGTGGTTATGAAAATGATGC15850.20570844170339567No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15240.19779158684919562No Hit
GGCTATGGACGAGTCTGACTTTTACTGTCAGTCGTGGGACCCTGACAGCGGCGCTGTGGTCTTCGGCAGGGGGAC14730.19117257705306112No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14340.1861109813266053No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14170.1839046447278938No Hit
GTAATAGACGGCCGTGTCCGCAGTGGTCATAGAGGTCACTTTCAGGGACAGTTCCTTCTTGCCCCAATCAAAAGA14000.1816983081291823No Hit
CTCCAGGACAGACAGCCATCATCACCTGCTCTGGAACTATTTTGCGGAGT13440.17443037580401502No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12950.16807093501949363No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12930.16781136600788052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12830.16651352094981492No Hit
ACCCAGGCTATGGACGAGTCTGACTTTTACTGTCAGTCGTGGGACCCTGA12740.1653454603975559No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCC12450.1615817097291657No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12410.16106257170593946No Hit
CTCTTACCATCAGCGCGACCCAGGCTATGGACGAGTCTGACTTTTACTGTCAGTCGTGGGACCCTGACAGCGGCG11480.1489926126659295No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11170.14496929298592617No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10960.14224381836398844No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10900.14146511132914907No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10720.13912899022463102No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10650.13822049868398512No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10430.13536523955624083No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10350.13432696350978834No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10320.13393760999236867No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10090.1309525663588178No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9540.12381441853945709No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9520.12355484952784397No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9460.12277614249300461No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9300.12069959040009967No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9250.12005066787106689No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9090.11797411577816194No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9000.11680605522590291No Hit
GCACAGGATGTGTGGCCTCCTCTGAACTGACTCAGCCGCTCTCAGTGTCC8880.11524864115622421No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8840.11472950313299796No Hit
TCCATAGCCTGGGTCGCGCTGATGGTAAGAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAAGCGCTCAGAGATC8690.11278273554589958No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8640.11213381301686678No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8530.11070618345299466No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8450.10966790740654217No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8420.1092785538891225No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8370.1086296313600897No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8160.10590415673815197No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8140.10564458772653887No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8100.10512544970331261No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8010.1039573891510536No Hit
AGCCTGGGTCGCGCTGATGGTAAGAGTGGCTGTGTTCCCAGACTTGGAGC7810.10136169903492243No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7710.10006385397685683No Hit
GATGGTAAGAGTGGCTGTGTTCCCAGACTTGGAGCCAGAGAAGCGCTCAGAGATCCCTGAGGGCCGCCTGGTAT7710.10006385397685683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)