FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700319
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30800.4397995770498873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30670.4379432801337676No Hit
GTTTAATAGTTACCCTCAGGGGGACACTTTTGGCCAGGGGACCAAGCTGG30290.43251718145587936No Hit
CTATTAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTC28090.401102925952316No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA22470.32085378234775863No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20410.2914386158307857No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19220.2744463594447673No Hit
GCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGAT19100.2727328545991184No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT18890.2697342211192328No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18150.25916760790439786No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18060.25788247927016117No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17020.24303210394120392No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16870.2408902228841428No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16760.2393195101089646No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16580.23674925284049128No Hit
GGCCAAAAGTGTCCCCCTGAGGGTAACTATTAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGC15640.22332679821624146No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT15450.22061374887729734No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14940.21333135328328945No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGGGCATTAGCAGTGCTTTAGGCTGGTATCAGCAGAAA14860.21218901671952356No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14450.2063345418302231No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14310.20433545284363272No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14090.20119402729327635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13550.19348325548785625No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT13430.19176975064220733No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12940.18477293918914095No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12510.178632880158899No Hit
GTGTATTACTGTGCGAGATATCGGGTGCTTCTAAGGGGGCGCTACTTTGA11990.1712076924944204No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG11730.1674950986621811No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11400.16278296033664658No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11380.16249737619570512No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11230.16035549513864394No Hit
GATTTCACTCTCACCATCAGCAGGCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTAATAGTTAC11130.15892757443393654No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10720.15307309954463608No Hit
GCATAAAGGAGGATGGAAGTGAGAAATTCCATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG10460.14936050571239678No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT10410.14864654536004307No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10330.14750420879627713No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTCCCCCTGAGGGTAACTATTAAA10280.1467902484439234No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9660.13793714007473737No Hit
GATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA9650.13779434800426663No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9600.1370803876519129No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9590.13693759558144217No Hit
GGGCATTAGCAGTGCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATGATGCCTC9420.13451013038343954No Hit
GCCTAAAGCACTGCTAATGCCCTGACTTGCCCGACAAGTGATGGTGACTC9300.13279662553779065No Hit
ACTTATTACTGTCAACAGTTTAATAGTTACCCTCAGGGGGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA9080.1296551999874343No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8980.12822727928272687No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA8840.1262281902961365No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8670.12380072509813385No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8540.1219444281820142No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8500.12137325990013122No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8500.12137325990013122No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8440.12051650747730677No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8410.12008813126589454No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8370.11951696298401157No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8310.11866021056118711No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8240.11766066606789191No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8220.11737508192695044No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8170.11666112157459672No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGG8130.11608995329271375No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7980.11394807223565262No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7920.11309131981282815No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC7890.11266294360141593No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7850.11209177531953296No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7590.10837918148729364No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC7590.10837918148729364No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7540.10766522113493994No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7530.1075224290644692No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7500.10709405285305697No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7470.10666567664164472No Hit
CCTTTATGCTGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC7380.10538054800740804No Hit
AGTCAGGGCATTAGCAGTGCTTTAGGCTGGTATCAGCAGAAACCAGGGAA7330.10466658765505434No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7290.10409541937317138No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTTAGTACCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG7280.10395262730270062No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA7220.10309587487987618No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG7210.10295308280940543No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7180.1025247065979932No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCATAGATCAGGAGCTTAGGAGCTTTCCCTGGTTTCTG7170.10238191452752246No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7110.10152516210469802No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7080.10109678589328577No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7070.10095399382281504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAC252.7259174E-454.5305943
GCCTATC200.007389028651.69460339
TAGACAG200.007721451651.1224335
ACGGGCT200.00772581251.1151316
AATGGTA2750.041.4874970
GTGGTAT1700.040.101751
GGGCGTA350.001430737938.950424
CGAACTA451.06178675E-437.9524829
GTATCAA16000.037.7081761
TCTGGAG7600.035.02781770
TCGTATG1201.8189894E-1234.4979440
ATCTCGT1102.910383E-1134.3761937
ATAGCGT400.002755662734.0816236
CGTATGC1253.6379788E-1233.1635741
TATACAC1550.032.9822123
ATGCCGT1305.456968E-1232.01549544
TATCAAC19200.031.5965022
TCTCCGA1307.2759576E-1231.45546311
ATCAACG19550.031.2051683
TCAACGC19700.030.9675624
Produced by FastQC (version 0.11.5)