FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700319
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAATAGTTACCCTCAGGGGGACACTTTTGGCCAGGGGACCAAGCTGG30040.4289473796941108No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28890.41252629158997545No Hit
CTATTAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTC27860.3978187083314889No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24630.3516968695694391No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20780.29672192243820317No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA19670.28087200261595074No Hit
GCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGAT18750.2677351321326424No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18310.26145228103192975No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT18150.25916760790439786No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17430.24888657883050438No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17060.24360327222308692No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16630.23746321319284497No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15610.22289842200482923No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15270.21804349160882397No Hit
GGCCAAAAGTGTCCCCCTGAGGGTAACTATTAAACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGC14950.21347414535376025No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT14390.2054777894073986No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14010.2000516907295104No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13980.19962331451809817No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13970.19948052244762746No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13940.1990521462362152No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13730.1960535127563296No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGGGCATTAGCAGTGCTTTAGGCTGGTATCAGCAGAAA13620.19448279998115145No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12980.18534410747102392No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT12170.17377794976289376No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG11940.1704937321420667No Hit
GTGTATTACTGTGCGAGATATCGGGTGCTTCTAAGGGGGCGCTACTTTGA11860.16935139557830076No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11650.16635276209841515No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11570.1652104255346492No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11540.16478204932323698No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11470.1637825048299418No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11320.16164062377288063No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10830.15464381231981425No Hit
GATTTCACTCTCACCATCAGCAGGCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGTTTAATAGTTAC10570.15093121848757496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10350.1477897929372186No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT9910.14150694183650592No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTCCCCCTGAGGGTAACTATTAAA9840.14050739734321074No Hit
GCATAAAGGAGGATGGAAGTGAGAAATTCCATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG9670.1380799321452081No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9520.135938051088147No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9250.13208266518543693No Hit
GGGCATTAGCAGTGCTTTAGGCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAGCTCCTGATCTATGATGCCTC9240.13193987311496616No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9090.12979799205790504No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA9070.12951240791696356No Hit
GCCTAAAGCACTGCTAATGCCCTGACTTGCCCGACAAGTGATGGTGACTC9020.12879844756460984No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8980.12822727928272687No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8960.12794169514178538No Hit
GATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA8560.12223001232295568No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8530.12180163611154346No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8310.11866021056118711No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGG8180.11680391364506745No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8050.11494761672894781No Hit
ACTTATTACTGTCAACAGTTTAATAGTTACCCTCAGGGGGACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA8000.11423365637659409No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7970.11380528016518186No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7970.11380528016518186No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7950.11351969602424038No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7900.11280573567188668No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7870.11237735946047443No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7840.11194898324906222No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7770.11094943875576702No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7680.10966431012153033No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC7530.1075224290644692No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGCCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG7500.10709405285305697No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7450.10638009250070325No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7400.10566613214834954No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTTAGTACCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG7370.10523775593693731No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7370.10523775593693731No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA7330.10466658765505434No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7310.10438100351411285No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7300.10423821144364212No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7290.10409541937317138No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7240.10338145902081765No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7180.1025247065979932No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7150.10209633038658096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATCG252.5524033E-455.2614140
CACTCGC200.007807682750.979017
GTGGTAT1800.045.363261
TCTGGAG6250.042.71115570
TTACGAC350.001389568139.1824534
GTATCAA15900.036.590171
CGATGTT400.002525943334.69193343
TTTACGA400.00268602834.25996833
GTTTACG400.002698374234.22794332
GAAATGT800.00344773432.55423470
TATCAAC17950.032.3882062
ATCAACG18150.031.837173
ATCCCGA450.003889895531.76563560
TCAACGC18250.031.6604584
CAACGCA18700.031.2598535
AAGCCGT450.004497668230.8372743
AACGCAG19000.030.766286
CCTCGAT450.004596350730.70078740
GTTTATC450.004622515830.66518639
ACGACCT450.00471835530.53682136
Produced by FastQC (version 0.11.5)