FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817457
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTTCCCCATTCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCAT59820.7317816105311961No Hit
GTTTAGGAGGTTGTCCTGGTTTCTGCTGGTACCAAGTTAAGTAGTCGTTAGTGTTGGAGCTGGAAAAAACACT55910.6839503484586956No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT50560.618503480917039No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48900.5981966023900952No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43800.5358079996868337No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC40470.49507191203941003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37240.4555591303273444No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34070.4167803321764937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32160.39341518881115456No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28860.35304609294433836No Hit
GTTTATTACTGTCAGCAATATTATAGTTCCCCATTCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAA28510.3487645221705851No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27900.34130235596490094No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27700.3388557440941848No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26300.3217294609991718No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25950.3174478902254186No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24540.30019927653686984No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24000.2935934244859363No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23300.2850302829384298No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22370.2736535377395998No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22250.2721855706171701No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22140.27083993408827617No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21550.2636224290696636No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21500.2630107761019846No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20820.2546922957415497No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTG20310.2484534354712236No Hit
CCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT20040.24515050944575678No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTTCC19850.24282622816857646No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTC19290.2359757149305713No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTCCAGCTCCAA19160.23438541721460576No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTCCAGCTCCAACA18650.22814655694427963No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGTTGTCCTGGTTTCTGCTGGTA18370.22472130032527704No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18170.22227468845456094No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18120.22166303548688185No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17520.21432319987473347No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17170.21004162910098026No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17010.2080843396044073No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16920.20698336426258507No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15990.19560661906375504No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15800.19328233778657472No Hit
GACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15650.1914473788835376No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15460.18912309760635726No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT15410.18851144463867825No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15190.18582017158089048No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15050.1841075432713892No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14670.17945898071702854No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14490.17725703003338403No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14460.1768900382527766No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14440.176645377065705No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14420.17640071587863337No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTCCAGCTCCAACACTAACGACTA14210.17383177341438144No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14170.17334245104023818No Hit
GTAAATGAGCAGTTTAGGAGGTTGTCCTGGTTTCTGCTGGTACCAAGTTA13930.17040651679537883No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13860.1695502026406282No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTTCCCCATTCACTTT13810.16893854967294916No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13770.16844922729880593No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13720.1678375743311269No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13620.16661426839576882No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13450.16453464830566011No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13340.16318901177676623No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13130.1606200693125143No Hit
ACTTAACTTGGTACCAGCAGAAACCAGGACAACCTCCTAAACTGCTCATT13060.15976375515776364No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12810.15670549031936848No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12690.15523752319693881No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12660.15487053141633136No Hit
CTACTTAACTTGGTACCAGCAGAAACCAGGACAACCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATC12600.15413654785511655No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCC12200.14924332411368427No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12120.1482646793653978No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTTCCCCAT11940.1460627286817533No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11910.14569573690114587No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11900.14557340630761006No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG11890.14545107571407426No Hit
AACCAGGACAACCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAA11820.1445947615593236No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11710.14324912503042975No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG11560.14141416612739263No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11550.14129183553385682No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG11410.13957920722435554No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11230.137377256540711No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG11170.1366432729794962No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11110.13590928941828131No Hit
TTATTACTGTCAGCAATATTATAGTTCCCCATTCACTTTCGGCGGAGGGA11090.1356646282312097No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10770.13175004923806388No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10750.1315053880509923No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10520.12869178439966872No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA10490.1283247926190613No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10420.12746847846431067No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10360.1267344949030958No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10300.12600051134188098No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10160.1242878830323797No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10080.12330923828409322No Hit
GGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10000.12233059353580678No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTGAATGGGGAA9980.12208593234873516No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9970.12196360175519935No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC9960.12184127116166354No Hit
GTTAAGTAGTCGTTAGTGTTGGAGCTGGAAAAAACACTCTGGCTGGACTT9930.12147427938105614No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9850.12049563463276969No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9750.11927232869741161No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAATGGGGAACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC9740.1191499981038758No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG9680.11841601454266096No Hit
GTGTTGGAGCTGGAAAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGC9650.11804902276205355No Hit
CCTTGGTCCCTCCGCCGAAAGTGAATGGGGAACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC9620.11768203098144613No Hit
CTCCAACACTAACGACTACTTAACTTGGTACCAGCAGAAACCAGGACAAC9450.11560241089133741No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC9330.11413444376890772No Hit
GCAGAAACCAGGACAACCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT9300.1137674519883003No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA9200.11254414605294223No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC9160.11205482367879901No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9150.11193249308526321No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9120.11156550130465578No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8940.10936355062101126No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG8890.10875189765333224No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8840.1081402446856532No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA8800.10765092231150995No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG8800.10765092231150995No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAAT8750.10703926934383093No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8580.10495964925372223No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT8520.10422566569250737No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT8500.10398100450543575No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8320.10177905382179125No Hit
CCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTCCAGCTCCAACACTAAC8230.10067807847996897No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG8210.10043341729289736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGT208.772624E-568.5820637
GTCTAGT150.002552160867.617121
ATCTCGT800.060.009337
GAGCGAT1200.059.6319132
CGTATGC1450.059.5361941
TCGTATG1400.056.6339440
ATGCCGT1550.055.99602544
CCGTCTT1500.055.77228547
CCCACGA1650.055.34679819
TGTCGTA252.6263265E-454.94405438
CGTCTTC1400.054.86979348
GCGATGT252.704629E-454.61825634
CACGAGA1700.053.7419721
TCTCCGA1750.052.1681511
GGTATCA8750.051.0026251
CCGGCTC200.00791181250.80919325
ACGAGAC1800.050.7625222
GCCGTCT1650.048.5405146
CGAGACA1900.048.09670323
GTATGCC1800.048.058842
Produced by FastQC (version 0.11.5)