FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612814
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24630.40191640530405637No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22540.36781144033915675No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA19030.3105346809961913No Hit
GTAATAGACGGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGAAGATACA18390.3000910553610068No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16490.26908654175655256No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16340.2666388169983062No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCG15710.25635837301367137No Hit
CAGTAATAGACGGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGAAGATA15400.25129974184662884No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14890.24297747766859112No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14020.2287806740707621No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13870.2263329493125157No Hit
CTCTAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCTCATGTACTTGGCAC13450.21947931998942583No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG13430.21915295668832632No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGCTGCCCTGTT13340.2176843218333785No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13300.21703159523117943No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13250.21621568697843063No Hit
CTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGCAGCAACTGGCCTCCGAGGTT12980.21180978241358717No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12370.20185570173005185No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12090.1972866155146586No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT12020.19614434396081026No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGCAGCAAC11420.18635344492782477No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11370.18553753667507597No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11310.1845584467717774No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11310.1845584467717774No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11110.18129481376078221No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGAACCTCGGAGGCCAGTTGCTGCG10950.17868390735198608No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10930.17835754405088655No Hit
GACTTCACTCTCTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGCAGCAACTGG10900.17786799909923728No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA10880.17754163579813778No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10760.17558345599154065No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10660.17395163948604309No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10610.17313573123329426No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGCGC10460.1706880064750479No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10090.1646502854047068No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGAACCTCGGAGG9960.16252892394755997No Hit
GGGCTAGAGTGGGTCTCAATTCTTTATAGCGATGGTAACACATATGCCGC9890.16138665239371164No Hit
GTTTATTACTGTCAGCAGCGCAGCAACTGGCCTCCGAGGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA9650.1574702927805174No Hit
GTCTATTACTGTGCGCGCGTGGGCGCCTCTTATTATGATAATAGTGGTCA9590.15649120287721885No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9360.1527380249145744No Hit
GTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTA9350.15257484326402465No Hit
ACATCAAACAGGGCAGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTC9150.14931121025302946No Hit
GTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATA9100.14849530200028066No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT9090.14833212034973092No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8840.14425257908598693No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8760.14294712588158887No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT8720.14229439927938983No Hit
GATCTGCACTGAACACAGAAGACTCGCCATGGAGTTTGGGCTGAGCTGGG8700.1419680359782903No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8510.1388675846178449No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8460.1380516763650961No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8390.1369094048112478No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8250.13462486170355117No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8180.13348259014970285No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8070.1316875919936555No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGC8070.1316875919936555No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7920.12923986723540912No Hit
GTGTGAGGTGGTACTGGTGGAGTCTGGGGGAGGCCTGGTCCAGCCGGGGGGGTCCCTTAGACTCTCCTGTGCAGC7870.12842395898266032No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7860.12826077733211055No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7820.12760805072991152No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7820.12760805072991152No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7810.12744486907936176No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7800.127281687428812No Hit
GTTTGATGTATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGTT7780.1269553241277125No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7780.1269553241277125No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7700.12564987092331442No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7650.12483396267056561No Hit
GCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGG7640.12467078102001586No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7600.12401805441781683No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGCAGTTGTGTTGACACAGTC7500.12238623791231923No Hit
GGCCAAAAGTGAACCTCGGAGGCCAGTTGCTGCGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGG7440.1214071480090207No Hit
GTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC7380.12042805810572213No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7310.11928578655187382No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7150.11667488014307768No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7140.1165116984925279No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7080.11553260858922937No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7030.11471670033648056No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT6950.11341124713208249No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCATCGTCAGTGCCAAGTAC6810.11112670402438586No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6800.11096352237383611No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6710.10949488751888828No Hit
CTCCCAGGCTCCTCATCTATGATACATCAAACAGGGCAGCTGGCATCCCA6680.109005342567239No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6680.109005342567239No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG6670.10884216091668925No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6660.10867897926613948No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6440.10508898295404479No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6390.10427307470129599No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6330.10329398479799742No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6330.10329398479799742No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6320.10313080314744767No Hit
GAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTC6310.10296762149689792No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6310.10296762149689792No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6250.10198853159359936No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6230.10166216829249985No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACCGGAGCAGTTGTGTTGACACAGTCTC6140.10019353343755201No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6140.10019353343755201No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6140.10019353343755201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA100.008752795135.1225170
TCGTATG1100.053.2711640
ATCTCGT1000.047.99874537
CGTATGC1250.046.929241
TTGATAT306.069286E-446.3630246
ACGAGAC1250.046.14787722
CGTAAAC306.6479604E-445.5141832
TCGTAAA306.672202E-445.4805731
GTCGTAA306.7181827E-445.4172330
GTATGCC1350.043.54331242
TCTGGAG6050.042.43517370
TATACAC1450.042.1159323
ATGCCGT1400.042.1002344
CTTATAC1300.041.7559661
GATCTCG1100.040.4483836
ATCTCCG1450.039.7729110
GTATCAA9950.039.5527651
GGTATCA3550.039.1829761
GGTCGTA350.001440243638.89718229
GAGACCG1400.038.79555524
Produced by FastQC (version 0.11.5)