FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612814
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23980.39130959801832205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19180.3129824057544377No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA18470.30139650856540484No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18050.294542879242315No Hit
GTAATAGACGGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGAAGATACA17800.290463337978571No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17030.27789835088623954No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16650.2716974481653487No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCG15730.25668473631477084No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14420.23530794009275244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14370.23449203184000367No Hit
CAGTAATAGACGGCCGTGTCTTCAGTTCTCAGGCTGTTCATTTGAAGATA13960.22780158416746354No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13260.2163788686289804No Hit
CTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGCAGCAACTGGCCTCCGAGGTT12900.2105043292091891No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGCTGCCCTGTT12880.21017796590808957No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12540.20462978978939778No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12440.20299797328390015No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12360.20169252007950209No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11610.1894538962882702No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT11590.18912753298717067No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11510.1878220797827726No Hit
CTCTAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCTCATGTACTTGGCAC11450.18684298987947404No Hit
GGGCTAGAGTGGGTCTCAATTCTTTATAGCGATGGTAACACATATGCCGC11330.18488481007287694No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11280.18406890182012814No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11210.1829266302662798No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGAACCTCGGAGGCCAGTTGCTGCG11170.18227390366408078No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA11150.18194754036298125No Hit
GACTTCACTCTCTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGCAGCAACTGG10930.17835754405088655No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGCGC10880.17754163579813778No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10750.1754202743409909No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10740.17525709269044115No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10500.17134073307724693No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10320.16840346336735126No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGCAGCAAC9910.16171301569481114No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9820.16024438083986334No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9630.1571439294794179No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9610.15681756617831838No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT9570.15616483957611935No Hit
GTTTATTACTGTCAGCAGCGCAGCAACTGGCCTCCGAGGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA9560.15600165792556958No Hit
GTCTATTACTGTGCGCGCGTGGGCGCCTCTTATTATGATAATAGTGGTCA9360.1527380249145744No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGAACCTCGGAGG9150.14931121025302946No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9130.14898484695192996No Hit
GTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTTCTTAGCCTGGTA9120.1488216653013802No Hit
GTGTGAGGTGGTACTGGTGGAGTCTGGGGGAGGCCTGGTCCAGCCGGGGGGGTCCCTTAGACTCTCCTGTGCAGC9090.14833212034973092No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8890.14506848733873573No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT8870.14474212403763623No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8790.14343667083323816No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8540.13935712956949417No Hit
GGCCAAAAGTGAACCTCGGAGGCCAGTTGCTGCGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGG8320.13576713325739947No Hit
ACATCAAACAGGGCAGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTC8280.13511440665520044No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8220.13413531675190188No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8200.13380895345080235No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8180.13348259014970285No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8000.13054532043980718No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCCTGC7930.12940304888595888No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7860.12826077733211055No Hit
GTTTGATGTATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGTT7650.12483396267056561No Hit
GTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC7480.12205987461121973No Hit
GTCAGAGTGTTAGCAGCTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATA7460.1217335113101202No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7440.1214071480090207No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7420.12108078470792116No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7380.12042805810572213No Hit
GATCTGCACTGAACACAGAAGACTCGCCATGGAGTTTGGGCTGAGCTGGG7340.1197753315035231No Hit
CTGCTACTCTGGCTCCCAGATATCACCGGAGCAGTTGTGTTGACACAGTC7200.11749078839582647No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7200.11749078839582647No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7190.11732760674527672No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7090.11569579023977912No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7050.11504306363758009No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6940.11324806548153274No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6900.1125953388793337No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6900.1125953388793337No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6890.11243215722878394No Hit
GCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGG6850.1117794306265849No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCATCGTCAGTGCCAAGTAC6780.1106371590727366No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6690.10916852421778876No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6580.10737352606174141No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG6580.10737352606174141No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6450.10525216460459455No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6430.10492580130349502No Hit
CTCCCAGGCTCCTCATCTATGATACATCAAACAGGGCAGCTGGCATCCCA6370.10394671140019646No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG6310.10296762149689792No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6280.10247807654524864No Hit
CTATTACTGTGCGCGCGTGGGCGCCTCTTATTATGATAATAGTGGTCATG6190.1010094416903008No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6190.1010094416903008No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC6190.1010094416903008No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6170.10068307838920129No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGCG252.8128782E-454.1838762
AATCCGC200.00791304250.8056766
TCTGGAG6150.045.8241870
GGTATCA4300.042.54541
TTTAAGT1003.3208744E-642.27280870
AAAACCG1054.643969E-640.25981570
AATGGTA2750.038.42982570
GTATCAA10850.038.406321
GTGGTGA3150.038.0231670
TGTTAGA5400.035.22733770
TATCAAC12300.033.864922
GTGGTAT1450.032.710511
TGGTATC1450.032.6971662
TCAACGC13150.031.418414
AACGCAG13450.030.9744646
CAACGCA13450.030.9694085
ATCAACG13350.030.9477213
CTAGAGA553.6298734E-430.7862954
GCAAAGT1153.7333486E-430.6324770
CGCAGAG13700.030.41428
Produced by FastQC (version 0.11.5)