FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503869
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37740.2509527093117818No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35930.2389170865281484No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACCGAAGTCATCTTCAGGGACA29100.19350089668714496No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28670.19064160508661326No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26940.17913794353098575No Hit
GTACTGGGACCAGGGGATCCGGATCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT26560.17661112769795775No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26400.17554720524194595No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACCGAAGTCATCTTCAGGGACACTTGATTCTTCGACGTGTCCA25690.17082604934339363No Hit
GTATTATTGTGCGAGAGGATTTCGCCCCGTTAGCTGGTCCCACGCGGAGTACTGGGACCAGGGGATCCGGATCAC25100.16690283528685013No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24610.163644572765314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24440.16251415515580148No Hit
GATCTACGCTGCATCCACTTTGCAATCCGGGGTCCCATCTCGATTCAGTGGCAGTGGAGCTGGGACGGATTTCA24200.16091827147178378No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24140.16051930055077937No Hit
ACTTATTACTGTCAAAAATATAACGATGCCCCTCGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAATCGAA23700.1575935137967469No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23050.153271328819199No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22750.1512764742141769No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22720.1510769887536747No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22460.14934811476265553No Hit
GCGTAGATCAGGGCCTTGGGAGGTTCTCCTGGTTTTTGCTGATACCAGGC21400.14229962849157737No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21380.14216663818457592No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21340.14190065757057296No Hit
GTAATAAGTAGCAACATCTTCCGGCTGCAGGCCGCTGATGGTGAGAGTGAAATCCGTCCCAGCTCCACTGCCA21330.14183416241707222No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21120.14043776419355675No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20560.13671403559751547No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20430.1358495986020059No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20290.13491866645299555No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20100.13365525853648155No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19670.13079596693594986No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTGGGAGACACAGTCACCATGACTT19640.13059648147544764No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19130.12720522864691006No Hit
GTGTATTATTGTGCGAGAGGATTTCGCCCCGTTAGCTGGTCCCACGCGGA19070.12680625772590565No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18900.12567584011639313No Hit
GTCATGGACCTCCTGCACAAGACCATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC18770.12481140312088353No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18720.12447892735337986No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG18560.12341500489736806No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18440.12261706305535922No Hit
GTTGAAGCCTTCGCAGACCCTGTCCCTCACCTGCGGTGTCTCTGGTGGGT18030.119890761761829No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17620.11716446046829876No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17490.11630002347278919No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17450.11603404285878624No Hit
GTGCAGCCACAGTTCGATTGATTTCCACCCTGGTCCCTTGGCCGAACGTC17420.11583455739828402No Hit
GGGCTGGAGTGGATCGGGGATATCAATCATCCCGGAACCATCAACTACAG17300.11503661555627517No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17290.11497012040277445No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATTTCCACCCTGGTCC17170.1141721785607656No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17150.11403918825376412No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17120.11383970279326192No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT16670.11084742088572876No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16280.1082541098992No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16200.1077221486711941No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGCCGGAAGATGTTGCTACTTATTACTGTCAAAAATATAACGATGCC16160.10745616805719115No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16110.10712369228968747No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15850.1053948182986683No Hit
GCACAAGACCATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTC15840.10532832314516756No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15790.10499584737766388No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15640.10399842007515281No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT15610.10379893461465062No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15600.10373243946114986No Hit
GTCCCAGTACTCCGCGTGGGACCAGCTAACGGGGCGAAATCCTCTCGCAC15510.10313398307964323No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15440.10266851700513809No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15200.10107263332112039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)