FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503869
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46000.30587770610339066No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35660.2371217173836285No Hit
GATCTACGCTGCATCCACTTTGCAATCCGGGGTCCCATCTCGATTCAGTGGCAGTGGAGCTGGGACGGATTTCA30950.20580250008478135No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30780.20467208247526877No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29340.19509678037116263No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACCGAAGTCATCTTCAGGGACA27550.1831941478945307No Hit
GTATTATTGTGCGAGAGGATTTCGCCCCGTTAGCTGGTCCCACGCGGAGTACTGGGACCAGGGGATCCGGATCAC26520.17634514708395477No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25000.16623788375184273No Hit
GTACTGGGACCAGGGGATCCGGATCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT24710.16430952430032136No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACCGAAGTCATCTTCAGGGACACTTGATTCTTCGACGTGTCCA24440.16251415515580148No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24180.1607852811647823No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23930.1591229023272639No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22770.15140946452117837No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22170.14741975531113416No Hit
ACTTATTACTGTCAAAAATATAACGATGCCCCTCGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAATCGAA22160.14735326015763342No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21920.14575737647361572No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21560.14336355094758918No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21510.1430310751800855No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20930.13917435627704275No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20640.13724599682552138No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20580.13684702590451694No Hit
GTGTATTATTGTGCGAGAGGATTTCGCCCCGTTAGCTGGTCCCACGCGGA20390.13558361798800295No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20220.1344532003784904No Hit
GCGTAGATCAGGGCCTTGGGAGGTTCTCCTGGTTTTTGCTGATACCAGGC20030.1331897924619764No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19790.1315939087779587No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTGGGAGACACAGTCACCATGACTT19550.12999802509394104No Hit
GTAATAAGTAGCAACATCTTCCGGCTGCAGGCCGCTGATGGTGAGAGTGAAATCCGTCCCAGCTCCACTGCCA19060.1267397625724049No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19050.12667326741890417No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18910.12574233526989387No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18580.12354799520436953No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18170.1208216939108393No Hit
GTTGAAGCCTTCGCAGACCCTGTCCCTCACCTGCGGTGTCTCTGGTGGGT18000.11969127630132678No Hit
GGGCTGGAGTGGATCGGGGATATCAATCATCCCGGAACCATCAACTACAG17930.1192258102268216No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17680.11756343138930318No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17600.11703147016129731No Hit
GTCATGGACCTCCTGCACAAGACCATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC17570.11683198470079509No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17470.11616703316578772No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17270.11483713009577297No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17270.11483713009577297No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16980.1129087706442516No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16750.11137938211373463No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16420.10918504204821032No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16390.10898555658770812No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG16320.10852009051320294No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16270.10818761474569927No Hit
GTGCAGCCACAGTTCGATTGATTTCCACCCTGGTCCCTTGGCCGAACGTC16100.10705719713618672No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16070.10685771167568452No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT16010.10645874075468009No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15770.10486285707066241No Hit
GTCCCAGTACTCCGCGTGGGACCAGCTAACGGGGCGAAATCCTCTCGCAC15640.10399842007515281No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15540.10333346854014544No Hit
GATTTCACTCTCACCATCAGCGGCCTGCAGCCGGAAGATGTTGCTACTTATTACTGTCAAAAATATAACGATGCC15530.10326697338664471No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15420.10253552669813659No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15400.10240253639113514No Hit
GCACAAGACCATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTC15300.10173758485612777No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15240.10133861393512335No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTGATTTCCACCCTGGTCC15160.10080665270711744No Hit
GCCCTGATCTACGCTGCATCCACTTTGCAATCCGGGGTCCCATCTCGATT15140.10067366240011597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT200.007707652651.14842231
GTGGTAT5300.039.025231
GTATCAA36600.035.7600141
GATTCAC2250.031.6466881
ATCAACG41200.031.165373
TCAACGC41400.031.0137834
TATCAAC42000.030.8995382
CAACGCA42100.030.5785835
AACGCAG42500.030.4491966
CGCAGAG44800.028.5834878
AGAGTAC44950.028.1866411
CAGAGTA45700.027.87231410
GCAGAGT46350.027.7007089
GACAGGT2450.026.2953531
AAAACCG2451.515109E-724.3775670
TCGGTTT1651.5350014E-424.1313270
ACGCAGA54400.023.9752587
GAGTACG39950.023.91218812
AGTACGG40500.023.7539513
GTCGGTT1101.7166742E-423.73808369
Produced by FastQC (version 0.11.5)