FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499301
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21930.43921402120163994No Hit
CTTTCATACAGCTCCACCAATAAGAACTACTTAGCTTGGTACCAGCAGAA19070.38193394365322725No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19040.38133310367894313No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14960.29961886717631253No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12930.25896202891642517No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12660.2535544691478687No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12580.2519522292164446No Hit
CTCCTAAGCTACTCATTTACTGGGCATCTACCCGTGAATCCGGGGTCCCT12380.24794662938788428No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12220.24474214952503598No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11940.23913430976505154No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11930.23893402977362352No Hit
GTTTATTACTGTCAGCAATATTATAATGTTCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAA11490.2301217101507908No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11100.22231079048509816No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10930.2189060306308219No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10720.21470015081083352No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10420.20869175106799304No Hit
ATATTATAATGTTCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT10200.2042855912565767No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTCATA9870.19767635153945218No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA9580.1918682317880397No Hit
GTCCCTGACCGATTCAGTGGCAGCGAGTCTGGGACAGATTTCACTCTCAC9550.19126739181375563No Hit
AACCAGGACAGCCTCCTAAGCTACTCATTTACTGGGCATCTACCCGTGAA9520.19066655183947157No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9510.19046627184804357No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9370.18766235196805134No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9280.18585983204519918No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9200.18425759211377507No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT8870.1776483523966505No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGGTGGAGCTGTATGAAAGACT8730.1748444325166583No Hit
GTATGAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA8710.17444387253380225No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC8630.17284163260237814No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTCATACAGCTCCAC8600.17224079262809408No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8530.17083883268809796No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8490.17003771272238588No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCA8430.1688360327738178No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG8360.1674340728338217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8340.16703351285096565No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8300.16623239288525357No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC8040.16102511310812515No Hit
GTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC7760.1554172733481407No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC7710.15441587339100063No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATGTT7500.15020999357101228No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7490.15000971357958426No Hit
ATTCAGTGGCAGCGAGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC7450.14920859361387218No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7150.1432001938710317No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7010.1403962739910395No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC6850.1371917941281912No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6840.1369915141367632No Hit
GTCAGGGACCCCGGATTCACGGGTAGATGCCCAGTAAATGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA6740.13498871422248304No Hit
ATTATAATGTTCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAG6710.13438787424819898No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6690.13398731426534297No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6550.1311833943853507No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTAC6480.1297814344453546No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT6470.1295811544539266No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6420.12857975449678652No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTACATTTACTCTCAGTAATGCCTGG6410.1283794745053585No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6290.1259761146082223No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6180.1237730347025141No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACTCATTTACTGGGCATCTACCCGTGAATC6160.12337247471965807No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATGTTCCTCGGACGTT6050.1211693948139499No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTCATACAGCTCCACCAATAAGAACTA5990.11996771486538181No Hit
GTAAATGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA5960.11936687489109775No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGGTGGAGCTG5880.11776463495967363No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5860.11736407497681758No Hit
ACTCATTTACTGGGCATCTACCCGTGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGAGTCTGGGACAGATTT5830.11676323500253354No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5790.11596211503682148No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5770.11556155505396545No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5710.11435987510539733No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5680.1137590351311133No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT5640.11295791516540124No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT5530.11075483525969304No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5500.110153995285409No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5480.10975343530255297No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5450.10915259532826892No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5450.10915259532826892No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5400.10815119537112884No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5340.10694951542256075No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACTCATT5310.10634867544827668No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5310.10634867544827668No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCCGAGGAACATT5260.10534727549113661No Hit
CTCCTGTGCAGCCTCTACATTTACTCTCAGTAATGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG5250.1051469954997086No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5240.10494671550828058No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5170.10354475556828446No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATGTTCCTC5140.10294391559400043No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC5060.10134167566257628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCC200.00614747754.153366
TCGTGTG200.00715007152.12392845
GACAGGT200.007624962451.2831341
GTACTGG200.007631005351.272866
CGAGCTA200.007631005351.2728623
CGGCGTG200.007634028351.26772714
AATGGTA1351.0913936E-1147.61139370
GGTATCA3550.047.190121
AATTGCC350.001161917540.63203461
GGGACTA350.001230773440.1592354
TAGTGTA350.001258443739.97809251
CTCTTAT350.001408448239.0728651
GTATCAA8600.035.7789341
CGTCTAT400.002335666735.2482256
TGCCGTC709.188898E-734.74928745
GACAGGC807.667768E-834.188761
CTTATAC701.043547E-634.188761
GTGGTAT701.043547E-634.188761
CAATACA400.002715507834.1819084
TATCAAC9450.032.55422
Produced by FastQC (version 0.11.5)