FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432517
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAGCAATAAGAACTACTTAGCTTGGTACCAGCAGAA64500.4502564367473475No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT61100.4265219889188051No Hit
GTTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGCTGGAGCTGTATAAAACACT48330.33737819516277995No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45380.31678507131154465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38610.26952559725294706No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAAGGAGTCTGGAGA37210.2597525893235473No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35410.247187293414319No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33560.23427296150761212No Hit
CTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAG27020.18861905303741597No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26990.18840963143892883No Hit
GTTTATTACTGTCAGCAATATTATAACGTCGGGTGGACGTTTGGCCAAGGGACCAAGGTGGAAATCAAACGAA26720.18652483705254458No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25970.18128929709036612No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25870.180591225095409No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25500.17800835871406762No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24560.1714464819614706No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23750.16579209880231788No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23650.16509402680736074No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23580.16460537641089076No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23400.16334884681996792No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23190.16188289563055797No Hit
ATATTATAACGTCGGGTGGACGTTTGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23160.16167347403207083No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23150.1616036668325751No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22340.15594928367342237No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCAAACGTC21760.15190046610267105No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20670.14429148135763833No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20500.14310475896621122No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19900.13891632699646847No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19750.13786921900403276No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTA19290.13465808782722996No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18470.12893389746858153No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18290.12767736787765868No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17890.12488507989783018No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17810.12432662230186448No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17740.12383797190539449No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCGTGGTTTCTGCTGGTA17710.12362855030690735No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17540.12244182791548022No Hit
GTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA17270.120557033529096No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16880.11783455274876319No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16820.1174157095517889No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16270.11357631357952472No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAACGTCGGGT16070.11218016958961045No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15940.11127267599616618No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAGCAATAAGAACTA15620.10903884561230337No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15590.10882942401381625No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15590.10882942401381625No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC15440.10778231602138054No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15380.10736347282440627No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15310.10687482242793629No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15270.10659559362995344No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT14810.10338446245315065No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14460.1009412104708007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3150.044.2815481
GGTATCA11600.043.9929161
CGTATGC1350.040.9184741
TGGTATC3500.040.8254282
TCGTATG1400.039.39847640
GACAGGT2650.038.5145531
GTATCAA27900.035.240591
GTAGTTA400.002376499435.12849852
ATCTCGT1800.032.427737
TAAGGCG2000.032.39589729
TCAACGC31300.031.5212274
TATCAAC31150.031.4545842
ATCAACG31450.031.370893
CTCGCTA553.53557E-430.92835412
CAACGCA32200.030.7458555
AACGCAG32750.030.3355146
CTAGGTA450.004950125230.2410564
AATGGTA5350.030.21465170
GTATGCC1850.029.90603442
AATCTCG1950.029.8974536
Produced by FastQC (version 0.11.5)