FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687178
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCACAA45990.6692589110827181No Hit
GTATAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA37540.5462922270503421No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29190.4247807700479352No Hit
GCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAGT28050.40819118190628917No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25230.3671537796611649No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24410.3552209180154196No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTATTCGGGAACCCGGGGTCCCT23460.3413962612307146No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTATTCGGGAA22050.3208775601081525No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT19800.2881349519338512No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19280.2805677713780127No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19280.2805677713780127No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18980.27620209028810583No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCA18470.2687804324352642No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18220.2651423648603419No Hit
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG18000.2619408653944102No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16700.24302291400481388No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16620.24185873238083874No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16270.2367654377759474No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16260.23661991507295055No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15970.23239975668604057No Hit
GTAATATACGGCCGTGTCGTCGGCTCTCAGACTGTTCATTTGCAGAAACA15970.23239975668604057No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15750.22919825722010892No Hit
GTCCCAGACCCGGTGCCACTGAATCGGGCAGGGACCCCGGGTTCCCGAAT15440.22468705342720519No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15160.22061241774329216No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14570.21202657826647534No Hit
ATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC14370.20911612420653744No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC14330.20853403339454987No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13210.1922354906588977No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13190.19194444525290388No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATA12980.18888846848996912No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12860.18714219605400637No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGGCAGGGACC12260.1784108338741927No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12230.17797426576520203No Hit
ATATTATATTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT12010.17477276629927035No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCA11590.16866081277340078No Hit
GTATATTACTGTGCGAAGTTTCGCTACGGTGATTACGTCCCTTTTGACAA11580.1685152900704039No Hit
GAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGGCAGGGACCCCG11540.16793319925841632No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11470.16691454033743805No Hit
ATATTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA10990.15992945059358712No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10860.15803765545462747No Hit
GTTTATTACTGTCAGCAATATTATATTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10410.15148913381976722No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10370.15090704300777966No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAA10270.1494518159778107No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10240.14901524786882003No Hit
CCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9500.1382465678470498No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT9360.1362092500050933No Hit
GAATCGGGCAGGGACCCCGGGTTCCCGAATAGAAGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTGTG9330.13577268189610261No Hit
GTACCAGCACAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTATTCGGGAACCCGGGGTCCCTGC9260.13475402297512434No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9250.13460850027212745No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG9180.13358984135114918No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9150.1331532732421585No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9140.1330077505391616No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9060.13184356891518648No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9050.13169804621218956No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9000.1309704326972051No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8770.12762341052827653No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8760.12747788782527963No Hit
ATTCAGTGGCACCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC8690.12645922890430136No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8610.1252950472803262No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC8580.12485847917133551No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8520.12398534295335414No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8370.12180250240840074No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8310.12092936619041937No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8290.12063832078442557No Hit
CTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC8270.12034727537843179No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8250.12005622997243799No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8230.11976518456644422No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8220.11961966186344732No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAACAATAAGAACTA8140.11845548023947217No Hit
GTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAG8100.11787338942748457No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8040.11700025320950322No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8030.11685473050650633No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8020.11670920780350942No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8020.11670920780350942No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7990.11627263969451875No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7930.11539950347653738No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7890.1148174126645498No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7850.11423532185256222No Hit
CCACTGAATCGGGCAGGGACCCCGGGTTCCCGAATAGAAGCCCAGTAAAT7770.11307114022858705No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTA7740.11263457211959638No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGG7710.1121980040106057No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7640.11117934508962743No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG7520.1094330726536647No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA7490.10899650454467402No Hit
GCACAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTATTCGGGAACCCGGGGTCCCTGCCCGATT7450.10841441373268643No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7330.10666814129672371No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7300.106231573187733No Hit
GGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAA7270.10579500507874233No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG7190.10463082345476718No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACT7110.10346664183079203No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7080.10303007372180134No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT7010.10201141480082308No Hit
TCTGAGGAGTAATATAATATTGCTGACAGTAATAAACTGCCACATCTTCA6970.10142932398883549No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATATTACTCCTCAGACGTT6960.10128380128583861No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC6880.10011961966186346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA150.002650932266.9713828
ATCGCGG200.00767806151.1951237
CTTATAC5150.046.552631
GTGGTAT1300.046.1050151
TGGTATC1300.046.1050152
TATACAC5550.043.197493
GTATGCC5150.043.0857442
TATGCCG5100.042.9552743
ATGCCGT5050.042.81236644
AATCGCG405.370487E-542.605436
TCGTATG4500.042.110340
CGAATCT5150.041.49447634
GCGAATC5650.041.31733333
ATCTCGT4800.041.24051737
CGTATGC4700.041.16612241
TAAGGCG5900.040.9237929
GGCGAAT5950.040.82824332
AGACTAA5900.040.7030525
AGGCGAA6050.040.62122731
AAGGCGA6050.040.55558830
Produced by FastQC (version 0.11.5)