FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687178
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49320.7177179711806839No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCACAA44740.6510685732081062No Hit
GTATAAAACAGTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA33760.491284645317516No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25700.373993346702019No Hit
GCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAGT25630.3729746877810407No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24790.36075078072930156No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTATTCGGGAACCCGGGGTCCCT24090.35056419151951895No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23950.3485268736775624No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTATTCGGGAA19580.2849334524679195No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19130.27838493083305926No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18800.2735826816341617No Hit
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG17530.2551012983535561No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCA17400.2532095032145965No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17130.24928039023368034No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16850.2452057545497673No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16420.23894827832090085No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15760.2293437799231058No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15260.2220676447732611No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15140.22032137233729834No Hit
GTCCCAGACCCGGTGCCACTGAATCGGGCAGGGACCCCGGGTTCCCGAAT14780.21508255502941015No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC14750.21464598692041947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14640.2130452371874536No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14470.2105713512365064No Hit
ATATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC14330.20853403339454987No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14300.20809746528555917No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14120.2054780566316151No Hit
GTAATATACGGCCGTGTCGTCGGCTCTCAGACTGTTCATTTGCAGAAACA13790.2006758074327176No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13520.19674669445180143No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13420.19529146742183248No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATA13240.19267205876788837No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12880.18743324146000018No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12490.1817578560431213No Hit
ATATTATATTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT12390.18030262901315236No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12210.17768322035920822No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGGCAGGGACC11180.1626943819505281No Hit
GAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGGCAGGGACCCCG11140.16211229113854053No Hit
GTATATTACTGTGCGAAGTTTCGCTACGGTGATTACGTCCCTTTTGACAA11050.16080258681156848No Hit
GTTTATTACTGTCAGCAATATTATATTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10680.15541824680068336No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10500.15279883814673928No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCA10420.15163465652276412No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10330.15032495219579206No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAA9960.14494061218490698No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9900.1440674759669256No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9870.14363090785793492No Hit
ATATTACTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA9770.14217568082796597No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9710.14130254460998462No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC9600.13970179487701875No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9430.13722790892607153No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9140.1330077505391616No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9120.13271670513316783No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9110.13257118243017094No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT8960.13038834188521753No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8660.1260226607953107No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8650.12587713809231377No Hit
GTACCAGCACAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTATTCGGGAACCCGGGGTCCCTGC8640.1257316153893169No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8620.1254405699833231No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8570.12471295646833862No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG8440.122821161329379No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8320.12107488889341626No Hit
CCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG8280.1204927980814287No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8270.12034727537843179No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8020.11670920780350942No Hit
GAATCGGGCAGGGACCCCGGGTTCCCGAATAGAAGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTGTG7990.11627263969451875No Hit
GTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAG7960.11583607158552807No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7910.1151084580705436No Hit
ATTCAGTGGCACCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC7860.11438084455555912No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7670.11161591319861812No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7610.11074277698063675No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7580.11030620887164608No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7510.1092875499506678No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7510.1092875499506678No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG7500.1091420272476709No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT7490.10899650454467402No Hit
CTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC7370.10725023210871128No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7360.10710470940571439No Hit
GGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAA7350.10695918670271748No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGGTGCCACTGAATCGGG7350.10695918670271748No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7340.1068136639997206No Hit
GCCCGATTCAGTGGCACCGGGTCTGGGACAGATTTCACTCTCACCATCAG7330.10666814129672371No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7310.10637709589072991No Hit
CCACTGAATCGGGCAGGGACCCCGGGTTCCCGAATAGAAGCCCAGTAAAT7250.10550395967274853No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA7230.10521291426675476No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7190.10463082345476718No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGACTGTTTTATACAGCTCCAACAATAAGAACTA7150.10404873264277958No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7150.10404873264277958No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7130.10375768723678581No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCTGAGGAGTAAT7060.10273902831580756No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6940.10099275587984481No Hit
GTCCCTGCCCGATTCAGTGGCACCGGGTCTGGGACAGATTTCACTCTCAC6900.10041066506785723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTA200.00828580950.21803329
GTGGTAT1050.044.2938041
GTCACCG4300.042.74029570
GGTATCA4600.042.6087151
GACAGGT851.0004442E-1039.0827671
TGGTATC1350.036.8980752
GTATCAA9800.034.5762861
GAGTTTA2150.033.992921
ATTACCA502.107487E-433.7760332
TACAAGG400.003129515833.2082672
GGATGCA450.003632991832.21175855
CGCCAGT553.754304E-430.61033231
CAGACCT500.004959364430.22672764
GCTTGTT554.0630446E-430.2003231
ATTCACT1002.0994776E-829.887442
TTTGAGG604.4205066E-429.76829751
GTATTAG450.00555951229.5292031
TATCAAC11250.029.5184572
TCTCGCC10500.029.41291642
TGTTAGA9000.029.05974870
Produced by FastQC (version 0.11.5)