FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651283
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC22660.34792862703310234No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG19000.29173185850083605No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18550.2848224197468689No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAGCACCCA17290.26547599123576077No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16230.2492004243930826No Hit
CATTATTACTGCCTCTCATATACAACCGGTGGCAATTTTTGGGTGCTCGG15980.2453618473075453No Hit
GAAATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA15510.23814532238673514No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15410.23660989155252019No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAGCACCCAAAAATTGCCA14540.22325164329485034No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14500.22263747096116435No Hit
CTCATATACAACCGGTGGCAATTTTTGGGTGCTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14370.22064141087668496No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT14250.21879889387562704No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13810.21204299820508135No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC13070.20068081003189092No Hit
GTAATAATGAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12960.1989918361142545No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12930.19853120686399No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAAC12720.19530680211213866No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12420.19070050960949386No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACCATGTCTCCTGGTACCAACAGCA12150.18655484635711359No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12150.18655484635711359No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12110.18594067402342762No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11910.18286981235499775No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTCATTATTACTGCCTCTCATATACAACCGGTGGCAATTTTTGGGTG11850.18194855385446881No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11750.18041312302025386No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11670.17918477835288193No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11370.17457848585023714No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11310.1736572273497082No Hit
GTATATGAGAGGCAGTAATAATGAGCCTCGTCCTCAGCCTGGAGCCCAGA11280.1731965980994437No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11130.17089345184812132No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10630.1632162976770467No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10560.16214149609309625No Hit
GTATATTTCTGTGTGAGAGATCACCAATGGGCCTTTGACCTCTGGGGCCA10390.15953126367493087No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10380.15937772059150937No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10230.15707457434018698No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10140.15569268658939356No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10050.15431079883860013No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10030.15400371267175714No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT9920.15231473875412072No Hit
GCTTATAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAACCCCCAAAGTCTTGATTTATGAAGTCACTAAT9890.15185410950385622No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9630.14786198933489741No Hit
GCACTGAACACAGAGGACGCACCATGGAGTTGGGGCTGTGCTGGGTTTTC9510.14601947233383952No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9500.14586592925041802No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8940.13726751657881445No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8830.13557854266117803No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8750.13435019799380607No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAACATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG8710.1337360256601201No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8280.12713367307299592No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAGCACCCAAAAATTGCCAC8170.1254446991553595No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8000.12283446673719413No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7840.12037777740245023No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7840.12037777740245023No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT7810.11991714815218577No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7760.11914943273507829No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7710.11838171731797084No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC7670.11776754498428488No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7630.1171533726505989No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7510.11531085564954098No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7490.115003769482698No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAGCAC7450.11438959714901203No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7430.11408251098216904No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7400.11362188173190456No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7400.11362188173190456No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7330.11254708014795411No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7320.11239353706453262No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7280.11177936473084665No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC7150.10978330464636724No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7150.10978330464636724No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6930.1064053568110944No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6870.10548409831056545No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6800.10440929672661502No Hit
CCCCTGAGGGCCGATTAGTGACTTCATAAATCAAGACTTTGGGGGTTTTGCCTGGGTGCTGTTGGTACCAGGAGA6790.1042557536431935No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6750.10364158130950754No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC6610.10149197814160664No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6570.10087780580792068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTA150.002465529468.2070859
TTCGCCC209.012736E-568.2070810
ACGCGTA1250.057.34678627
CGAGACG1250.057.3027523
GACGCGT1300.055.1284326
CGTAGTA1250.054.71269630
CGCGTAG1250.054.64118228
ACGAGAC1350.053.05402422
CGTATGC1250.052.55975341
GTAAACG200.007673595651.20248427
AGACGCG1400.051.1788925
AGTCGGC200.007696938251.16316623
ATCTCGT1150.050.88669637
ATGCCGT1300.050.72382444
GCGTAGT1350.050.6209329
GTAATCT1250.049.4240734
TCGTATG1350.048.60589640
CCCACGA1500.047.7449619
TCTCCGA1500.047.7449611
CCGTCTT1400.047.2854347
Produced by FastQC (version 0.11.5)