FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651283
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC22220.34117273136255666No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG19160.29418854783557996No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18620.2858972213308193No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAGCACCCA18000.2763775501586868No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16140.24781853664228914No Hit
CATTATTACTGCCTCTCATATACAACCGGTGGCAATTTTTGGGTGCTCGG15960.24505476114070227No Hit
GAAATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA15340.23553508996856976No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14950.22954690971513153No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14790.22709022038038765No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT14570.22371227254511483No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14410.2212555832103709No Hit
CTCATATACAACCGGTGGCAATTTTTGGGTGCTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14310.219720152376156No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13680.21004693812060196No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12870.19760994836346105No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12490.19177531119344432No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12390.1902398803592294No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12360.18977925110896493No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12300.18885799260843597No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAAC12240.187936734107907No Hit
GTAATAATGAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12170.18686193252395655No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12110.18594067402342762No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12090.18563358785658463No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAGCACCCAAAAATTGCCA11760.18056666610367536No Hit
GTATATGAGAGGCAGTAATAATGAGCCTCGTCCTCAGCCTGGAGCCCAGA11490.17642100285129506No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC11260.17288951193260071No Hit
GTATATTTCTGTGTGAGAGATCACCAATGGGCCTTTGACCTCTGGGGCCA11080.17012573643101386No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACCATGTCTCCTGGTACCAACAGCA11000.1688973917636419No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTCATTATTACTGCCTCTCATATACAACCGGTGGCAATTTTTGGGTG10990.16874384868022044No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10160.15599977275623655No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10100.15507851425570757No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10070.1546178850054431No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10060.1544643419220216No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9780.1501651355862198No Hit
GCTTATAACCATGTCTCCTGGTACCAACAGCACCCAGGCAAAACCCCCAAAGTCTTGATTTATGAAGTCACTAAT9680.14862970475200488No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9500.14586592925041802No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAGCAC9490.14571238616699653No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9480.14555884308357503No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9400.1443304984162031No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT9200.14125963674777323No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9060.13911003357987234No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8980.1378816889125004No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8880.13634625807828546No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8730.13404311182696307No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8550.13127933632537622No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8530.13097225015853325No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAACATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG8380.12866910390721084No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8320.12774784540668188No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC7900.12129903590297919No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7850.12053132048587174No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7730.11868880348481381No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7710.11838171731797084No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC7670.11776754498428488No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7660.11761400190086337No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC7640.11730691573402038No Hit
GCACTGAACACAGAGGACGCACCATGGAGTTGGGGCTGTGCTGGGTTTTC7580.11638565723349142No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7430.11408251098216904No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7300.11208645089768962No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7290.11193290781426814No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC7220.1108581062303177No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAGCACCCAAAAATTGCCAC7110.10916913231268127No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6920.10625181372767292No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6850.10517701214372246No Hit
CCCCTGAGGGCCGATTAGTGACTTCATAAATCAAGACTTTGGGGGTTTTGCCTGGGTGCTGTTGGTACCAGGAGA6820.10471638289345798No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6780.10410221055977202No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6730.10333449514266456No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6700.10287386589240008No Hit
GTCTTGATTTATGAAGTCACTAATCGGCCCTCAGGGGTCTCTGATCGCTT6690.1027203228089786No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6560.10072426272449918No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6560.10072426272449918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTACG150.002472152268.160943
TTACGCG150.00247290568.1557165
ACGATTA200.007159137652.10903545
AACGATT200.007190210752.05199444
GTACTAG200.007698820451.1599961
GTTACGC200.00772458251.1167874
GGTATCA6500.041.9774321
AATGGTA2600.038.43527270
GTATCAA15650.037.048771
TCTGGAG7650.034.83458370
ATGGCAA1359.611758E-734.54429270
TATCAAC16950.034.188882
CGGGATT400.002753901834.0857115
GTGGTAT1800.032.211851
ATCAACG18550.031.2327773
GTGTTAG553.481873E-431.0060561
TCAACGC18850.030.7333454
CAACGCA19200.029.9956135
AACGCAG19200.029.6406366
TGGTATC2000.028.9750792
Produced by FastQC (version 0.11.5)