FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064776
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62220.5843482572860395No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42980.40365297489800667No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATACTAATGGATACAACTATTTGG39990.3755719512836503No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39170.3678708009947632No Hit
GTCTCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGA38150.3582913213671232No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37420.351435419280675No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37380.3510597534129244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32210.30250494000616096No Hit
CTACAAACCCCTAACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACG31840.299030030729468No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31410.2949916226511492No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGATACAACTATTTGGCTTGGTACCTGCAAAAGGCAGGGCAGCCTC29660.27855624093706094No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTG29340.2755509139950562No Hit
GCCTGAGACAGTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAG28090.2638113556278504No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28050.2634356897600998No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27660.25977294754953156No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGACAGTGCCA27480.2580824511446539No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGATCCAGGGAGA26520.24906647031863982No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26400.24793947271538802No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT25680.24117748709587744No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25590.24033223889343863No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT24220.22746568292298097No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24030.2256812700511657No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23900.22446035598097627No Hit
GTCCCTGACAGGTTCAGTGGCACTGTCTCAGGCACAGATTTTACACTGAA23480.2205158643695951No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22810.21422346108477278No Hit
GTGTATTACTGTGCGAGAGATTCTCCCCTTTTTGGAGTGCTTACAGGTCG22660.21281471408070804No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG22520.21149988354358099No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22420.21056071887420452No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22380.21018505300645393No Hit
CCCCTAACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC22230.20877630600238925No Hit
CAGTGGCACTGTCTCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTA21570.20257781918450452No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21360.20060557337881393No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21280.19985424164331278No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGGCTGCCCTGCCTTTTGCAGGTA21150.19863332757312338No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20910.1963793323666198No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20850.19581583356499394No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20380.19140175961892453No Hit
GTCTCTGGTGTCTCCATCGGGAGTTACCACTGGAGTTGGATCCGGCAGCT20360.19121392668504925No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC20170.18942951381323397No Hit
GTGTTAGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTC20040.18820859974304455No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA19980.18764510094141865No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19110.17947436831784339No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19100.17938045185090573No Hit
GGGTACACCAAGTACAACCCCTCTCTCGAGAGTCGACTCACCATATCAGTCGACACGTCCAGGAACCAGTTCTCC19040.17881695304927983No Hit
GTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGGCTGCC18890.17740820604521515No Hit
GTTTATTACTGCATGCAAGCTCTACAAACCCCTAACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAA18420.17299413209914574No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC18280.1716793015620187No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC18190.17083405335957985No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGACAGTGCCA18050.1695192228224528No Hit
CTAATGGATACAACTATTTGGCTTGGTACCTGCAAAAGGCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGTT17920.16829830875226337No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17910.16820439228532574No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17610.16538689827719633No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16770.15749791505443397No Hit
GGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT16650.15637091745118223No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACC16610.15599525158343164No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC16380.15383517284386575No Hit
TTATTACTGCATGCAAGCTCTACAAACCCCTAACACTTTTGGCCAGGGGA16200.1521446764389881No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA15650.1469792707574175No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15640.14688535429047989No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCGGGAGTTACCACTGGAGTTGGATCCGGCAGCTCCCAGGGAAGGGA15560.1461340225549787No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15550.14604010608804105No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT15520.1457583566872281No Hit
GTTCAGTGGCACTGTCTCAGGCACAGATTTTACACTGAAAATCAGCAGAG15300.14369219441459988No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15240.143128695612974No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA15120.14200169800972223No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15090.1417199486089093No Hit
GTGTAAAATCTGTGCCTGAGACAGTGCCACTGAACCTGTCAGGGACCCCG15050.1413442827411587No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14850.13946595340240578No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14840.13937203693546812No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC14610.13721195819590223No Hit
GGCCAAAAGTGTTAGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCA14300.1343005477208352No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14290.13420663125389753No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC14290.13420663125389753No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14250.13383096538614694No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14160.13298571718370814No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14060.13204655251433164No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14030.1317648031135187No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13890.13044997257639165No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACCCCTAACACTTTTGGCCAGGGGA13850.13007430670864106No Hit
AGTCAGAGCCTCCTGCATACTAATGGATACAACTATTTGGCTTGGTACCTGCAAAAGGCAGGGCAGCCTCCACAG13790.1295108079070152No Hit
GGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT13630.12800814443601283No Hit
CTATTACACTGGGTACACCAAGTACAACCCCTCTCTCGAGAGTCGACTCACCATATCAGTCGACACGTCCAGGAA13550.12725681270051167No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13430.1261298150972599No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13330.12519065042788344No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13130.12331232108913048No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13070.1227488222875046No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13020.12227923995281637No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12890.12105832588262695No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12880.12096440941568931No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12720.11946174594468695No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12660.11889824714306108No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT12590.11824083187449755No Hit
CAACTATTTGGCTTGGTACCTGCAAAAGGCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGC12580.1181469154075599No Hit
CCTGCATACTAATGGATACAACTATTTGGCTTGGTACCTGCAAAAGGCAG12410.1165503354696199No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12410.1165503354696199No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12080.11345109206067755No Hit
AAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT12040.11307542619292696No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11970.11241801092436343No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11900.1117605956557999No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11790.11072751451948579No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11660.10950660044929637No Hit
GCAAAAGGCAGGGCAGCCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT11590.10884918518073285No Hit
GTCCAGCTTGGTCCCCTGGCCAAAAGTGTTAGGGGTTTGTAGAGCTTGCA11530.10828568637910696No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11440.10744043817666814No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11330.10640735704035402No Hit
GCTTGGTCCCCTGGCCAAAAGTGTTAGGGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT11130.1045290277016011No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11130.1045290277016011No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11040.10368377949916227No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11020.10349594656528698No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11000.10330811363141168No Hit
GGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10860.10199328309428463No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG10820.10161761722653403No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10750.10096020195797051No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10720.10067845255715756No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10720.10067845255715756No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10680.10030278668940697No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10670.10020887022246933No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT10650.10002103728859403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACAC150.002471331368.169663
CGAAAGA350.00485118757.49427870
GTGGTAT1800.051.141661
TGGTATC1800.049.2405852
GGTATCA5350.042.060431
GTATCAA14500.039.972791
GACTGAT9700.039.4161870
ACACGGT451.0728394E-437.8880466
TGGATTG8250.036.58726570
CGACTGA7900.034.53443569
TATCAAC16900.033.887922
ATCAACG17350.033.3972433
TCAACGC17400.033.3012734
AATGGTA4050.033.12427570
CAACGCA17750.032.6522945
AACGCAG18000.031.825966
ACGCAGA18700.030.6418137
CGCAGAG18800.030.2988268
TCGACTG8100.030.09593468
AGAGTAC19050.029.73752411
Produced by FastQC (version 0.11.5)