FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355997
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41950.30936646614999885No Hit
CTCCTGCACTGGGACCAGCAGTGACATTGGTACTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGT36400.26843717205864026No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGAGAGTGTTGGCCCTTGTAAATGAGCTG35350.26069379209541027No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34080.25132798966369396No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCACCATCTCCTG32870.24240466608701935No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGAGAGTGTTGGCCCTTGTAAATGAGCTGCAGTAATAAT31420.23171142709017792No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30890.22780286387064277No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC30060.22168190637589907No Hit
GTCAATCACCATCTCCTGCACTGGGACCAGCAGTGACATTGGTACTTATAACTATGTCTCCTGGTACCAGCAGCA28960.21356979403346762No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27260.20103289314061906No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26420.19483818917003504No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25910.19107711890218046No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25690.18945469643369417No Hit
GATTATTACTGCAGCTCATTTACAAGGGCCAACACTCTCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT25060.18480866845575616No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24410.1800151475261376No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23340.172124274611227No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22940.1691744155776156No Hit
GGCCAACACTCTCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA22630.16688827482656673No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21630.15951362724253815No Hit
CTCCTGGACAGTCAATCACCATCTCCTGCACTGGGACCAGCAGTGACATT21560.15899740191165615No Hit
GTGTTGGCCCTTGTAAATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC21380.157669965346531No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21290.15700624706396843No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20220.14911537414905787No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19870.14653424749464786No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCAC19580.14439559969527954No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACGGCCAGGGAGG19410.1431419096059947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18620.13731593801461212No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTC18590.13709469858709128No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17930.1322274311816324No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17650.1301625298581044No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17560.12949881157554186No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGCTTTGTTTGGAGGGTGTG17190.12677019196945125No Hit
CCACCACACCCTCCAAACAAAGCAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGT16700.12315661465327726No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16600.1224191498948744No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16220.11961678381294354No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15680.11563447411756811No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15500.11430703755244297No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCAATCACCATCT15200.11209464327723438No Hit
CATTGGTACTTATAACTATGTCTCCTGGTACCAGCAGCACCCAGGCAAAGTCCCCAAACTCATAATTTATGATGT15160.11179965737387325No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14920.11002974195370638No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14800.10914478424362296No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14260.10516247454824751No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14230.10494123512072666No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATTT14080.10383503798312237No Hit
CTCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG13640.1005901930461498No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGAGAGTGTTGGCCC13560.10000022123942752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)