FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089802
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC52590.48256472276615386No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48850.44824656221955916No Hit
CCCATATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC45580.41824111168817824No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG41030.37649040834940656No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACA41000.37621512898673337No Hit
GATTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT39650.3638275576664385No Hit
GTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA39010.35795493126274314No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACACAGTAATAAT35760.3281330003064777No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34210.313910233235028No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33080.30354137724100344No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30480.27968383247599105No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG30330.27830743566262495No Hit
ATGTATTACTGTGCGAGATGTTATAGGGGCTACGGTGACTACGTTGACTA29620.2717924907460254No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT28960.26573634476721464No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC28090.25775324324969123No Hit
GTCTCTGGTGGCTCCATCAGCGATAGTAGTTACTACTGGGGCTGGATCCG25940.23802488892477716No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG25230.23150994400817762No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25230.23150994400817762No Hit
GTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG25210.23132642443306214No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25140.23068410592015798No Hit
ATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA24650.2261878763298287No Hit
CTACTAGAGACTGAGCCAGAGGTCAAGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGG24030.22049876950124883No Hit
CTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAA24010.22031524992613336No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23950.2197646912007869No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCAT23660.2171036573616125No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG21660.1987516998500645No Hit
GTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC21410.196457705161121No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG20930.1920532353583495No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20340.18663940789244285No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20150.1848959719288458No Hit
CAGTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA20100.18443717299105708No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT20000.1835195751154797No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19560.17948214446293914No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19290.17700463019888016No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19100.1752611942352831No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19060.17489415508505213No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18950.173884797421917No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18900.17342599848412832No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG18650.1711320037951848No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18530.17003088634449193No Hit
GATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACGGTCACA17900.16425001972835432No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCGATAGTAGTTACTACTGGG17880.16406650015323884No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17880.16406650015323884No Hit
GTATTACTGTGCGAGATGTTATAGGGGCTACGGTGACTACGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT17760.16296538270254596No Hit
ACTAGAGACTGAGCCAGAGGTCAAGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGGCTC17720.162598343552315No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17710.16250658376475727No Hit
CCCCTGGAGGGACGGTCACACTCACTTGTGGCTTGACCTCTGGCTCAGTC17520.16076314780116022No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17230.15810211396198576No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTAC17140.15727627587396611No Hit
CTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC17100.15690923672373513No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16910.15516580076013808No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16670.15296356585875232No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16550.15186244840805946No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16260.149201414568885No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT15920.14608158179192182No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC15690.14397110667809382No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15550.14268646965228546No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15430.14158535220159257No Hit
CACTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG15210.1395666368753223No Hit
CAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC15130.13883255857486038No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG15040.1380067204868407No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC14640.13433632898453113No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14550.13351049089651146No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14440.13250113323337634No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT13980.1282801830057203No Hit
CTCCATATCCGTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCT13920.12772962428037388No Hit
CTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAG13790.12653674704212325No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACGGTCACACTCACTTGTGGCTTGACCTCTGG13780.12644498725456552No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTACTAGAGACTGAG13760.12626146767945No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13720.12589442852921906No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGAT13400.1229581153273714No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13310.12213227723935173No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13170.12084764021354337No Hit
GGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC13050.1197465227628505No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13050.1197465227628505No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG13020.11947124340017728No Hit
ACCCATATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT12850.11791132701169571No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12570.11534205296007899No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12550.11515853338496351No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12540.11506677359740577No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12510.11479149423473255No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12460.11433269529694384No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA12420.11396565614671288No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC12230.11222222018311583No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12230.11222222018311583No Hit
CTATTATAGTGGGAGCACCCACTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACACGTCCAAGAA12170.11167166145776938No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12150.11148814188265391No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12070.110754063582192No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11990.11001998528173007No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC11910.10928590698126815No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11720.1075424710176711No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11630.10671663292965143No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC11570.10616607420430499No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11550.10598255462918953No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11430.10488143717849664No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAA11390.10451439802826568No Hit
AGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTTTGAGAAT11230.10304624142734185No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACC11150.10231216312687992No Hit
GATATGGAGACTCGACTCTTGAGGGACGGGTTGTAGTGGGTGCTCCCACT11140.10222040333932218No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11080.10166984461397574No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11020.10111928588862933No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10920.10020168801305192No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCA10900.10001816843793643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG800.060.3386540
AATCTCG1050.055.58640336
TACCAAC252.574762E-455.16676740
ATCTCGT1000.054.98575637
CGTATGC900.053.69410741
CACGGAT10900.053.45956870
ACTAAGG1350.053.12936827
TACGATA200.007685420651.18487522
TCTCGTA1150.050.8537638
GGCGAAT1300.050.01933732
AGACTAA1450.049.43575725
ACGAGAC1450.049.4198822
GGTATCA6650.048.740791
AGGCGAA1500.047.88901531
TAAGGCG1500.047.851629
TATACAC1650.047.5591963
CACGAGA1600.046.9151621
ATGCCGT1050.046.16924744
GTATGCC1050.046.0684242
CGAATCT1350.045.69686534
Produced by FastQC (version 0.11.5)